Recombinant Human LANCL1 Protein: Handling & Application Protocol

Recombinant Human LANCL1 Protein is a research-grade reagent designed to support investigations into LanC-like protein 1 biology, including its roles in cellular signaling, immune regulation, and metabolic processes.

Product Name

Recombinant Human LANCL1 Protein

SKU

rp369008

Protein Family

LanC-like (LANCL) family, homologous to bacterial LanC enzymes

Species Origin

Human (Homo sapiens)

Primary Research Applications

Signal transduction studies, binding assays, Western blot, ELISA, immunoprecipitation

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles

Overview of LANCL1 and Research Significance

LANCL1 (LanC-like protein 1) is a mammalian member of the LanC enzyme superfamily, originally identified by homology to bacterial lanthipeptide synthetase C (LanC) enzymes. In humans, LANCL1 is expressed ubiquitously, with particularly high levels reported in neural and immune tissues. The protein localizes primarily to the cytoplasmic face of the plasma membrane and the cytosol.

Emerging evidence positions LANCL1 as a receptor and signaling hub for abscisic acid (ABA), a plant hormone that also exerts immunomodulatory and metabolic effects in mammalian systems. LANCL1, together with the related family member LANCL2, has been implicated in ABA-dependent activation of cAMP/PKA and AMPK signaling cascades, influencing inflammatory responses, insulin sensitivity, and immune cell function.

Recombinant Human LANCL1 Protein (SKU: rp369008) from Aladdin Scientific enables researchers to study protein–ligand interactions, screen for modulators of LANCL1 activity, and establish in vitro assay systems relevant to immunology, metabolic disease, and neuroscience.

Reconstitution and Storage Protocol

Materials required: Sterile deionized water or the reconstitution buffer recommended on the product datasheet, microcentrifuge tubes (low-protein-binding recommended), and a calibrated micropipette.

Reconstitution steps:

1. Allow the lyophilized protein vial to equilibrate to room temperature for 10–15 minutes before opening to minimize condensation.
2. Briefly centrifuge the sealed vial (1,000 × g, 30 seconds) to consolidate the lyophilized cake at the bottom.
3. Add the appropriate volume of sterile reconstitution buffer (typically sterile PBS, pH 7.4, or as specified on the Certificate of Analysis) slowly along the inner wall of the vial. Do not inject directly onto the protein cake.
4. Gently swirl or rotate the vial to dissolve the protein. Do not vortex, as vigorous agitation can cause denaturation and aggregation.
5. Allow the solution to sit for 5 minutes at room temperature to ensure complete dissolution. The solution should appear clear to slightly opalescent.

Storage conditions:

• Short-term (up to 1 week): Store at 2–8°C.
• Long-term: Aliquot into single-use volumes and store at -20°C or -80°C to prevent activity loss from repeated freeze-thaw cycles.
• A carrier protein (e.g., 0.1% BSA) may be added to the reconstituted solution to enhance stability during long-term storage, unless this would interfere with downstream assays.

Note: Always consult the Certificate of Analysis (CoA) supplied with each lot for lot-specific reconstitution volumes and storage instructions.

Experimental Applications and Assay Guidance

Recombinant Human LANCL1 Protein is suitable for a range of in vitro biochemical and cell-based research applications. Recommended uses include, but are not limited to:

• Protein–ligand binding assays: LANCL1 may be used as a capture or detection protein in surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), or bead-based binding assays to characterize interactions with ABA or other putative ligands.
• Western blot (WB): Use as a positive control antigen to validate anti-LANCL1 antibodies or to calibrate detection sensitivity in cell/tissue lysate experiments.
• ELISA: Coat microplates with recombinant LANCL1 as a capture antigen for sandwich or competitive ELISA formats to quantify LANCL1 or its binding partners in experimental samples.
• Immunoprecipitation (IP) and Co-IP: Serves as a standard for confirming antibody specificity and pull-down efficiency in studies of LANCL1-interacting proteins.
• Functional/enzymatic assays: Investigate potential GTPase or other enzymatic activities attributed to LANCL family members in defined biochemical systems.

Working concentration guidance: Optimal concentrations vary by assay format and must be determined empirically by the end user. As a general starting point, 0.1–10 µg/mL is commonly used for ELISA and WB applications with recombinant proteins of this class.

Flag: Specific validated working concentrations for rp369008 are not provided in the source material and should be confirmed with Aladdin Scientific technical support or by consulting the product datasheet.

Quality Control and Safety Considerations

Quality control: Each lot of Recombinant Human LANCL1 Protein (rp369008) is manufactured and tested to meet defined quality specifications. Typical QC testing for recombinant proteins of this class includes:

• Purity assessment by SDS-PAGE and/or HPLC (commonly ≥85% or ≥95% purity, lot-specific — confirm on CoA)
• Identity confirmation by mass spectrometry or N-terminal sequencing
• Endotoxin testing by LAL (Limulus Amebocyte Lysate) assay
• Biological activity testing where applicable

Always request and review the Certificate of Analysis (CoA) for the specific lot received. Store CoA records for experimental reproducibility and regulatory compliance.

Safety: Recombinant Human LANCL1 Protein is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or veterinary applications. Handle as a potential biohazard in accordance with your institution's biosafety guidelines. Use appropriate personal protective equipment (PPE) including gloves and eye protection. Consult the Safety Data Sheet (SDS) supplied by Aladdin Scientific for disposal and first-aid information.

Flag: Specific purity values and endotoxin limits for rp369008 are not stated in the source material. Obtain confirmed values from the product CoA or Aladdin Scientific technical support.

Troubleshooting Common Issues

The following guidance addresses frequently encountered issues when working with recombinant proteins such as LANCL1:

Issue	Possible Cause	Recommended Action
Protein aggregation after reconstitution	Vortexing during reconstitution; reconstitution at incorrect temperature	Reconstitute gently by swirling; reconstitute at room temperature; add 0.1% BSA as carrier; centrifuge at low speed to remove aggregates before use
Low signal in ELISA or WB	Suboptimal protein concentration; degradation due to improper storage	Titrate protein concentration; verify storage conditions; check CoA activity data; use fresh aliquots
Multiple bands on SDS-PAGE	Partial degradation; glycosylation or post-translational modifications	Check lot CoA for expected band pattern; store at -80°C; avoid repeated freeze-thaw; use protease inhibitors during sample preparation
Inconsistent results between experiments	Freeze-thaw degradation; pipetting variability with viscous solutions	Pre-aliquot protein into single-use volumes; equilibrate aliquots to working temperature before use; use calibrated pipettes

For issues not resolved by the above steps, contact Aladdin Scientific technical support and provide your lot number and CoA details.

Frequently asked questions

What is LANCL1 and why is it relevant to biomedical research?

LANCL1 (LanC-like protein 1) is a conserved mammalian protein with homology to bacterial lanthipeptide synthetases. It has been identified as a mammalian receptor for abscisic acid (ABA) and is involved in cAMP/PKA and AMPK signaling, making it relevant to research in immunology, metabolic diseases such as type 2 diabetes, and neurological conditions.

How should I reconstitute Recombinant Human LANCL1 Protein (rp369008)?

Allow the lyophilized vial to equilibrate to room temperature, then briefly centrifuge before adding the recommended volume of sterile reconstitution buffer (typically sterile PBS, pH 7.4) gently along the vial wall. Swirl gently to dissolve — do not vortex — and allow 5 minutes for complete dissolution. Always refer to the Certificate of Analysis for lot-specific instructions.

What assay applications is Recombinant Human LANCL1 Protein validated for?

Recombinant Human LANCL1 Protein is suitable for use in protein–ligand binding assays (e.g., SPR, ITC), Western blot as a positive control antigen, ELISA as a capture or standard antigen, and immunoprecipitation assays. Optimal concentrations should be empirically determined for each specific assay format.

How should I store Recombinant Human LANCL1 Protein to maintain activity?

For short-term use (up to one week), store at 2–8°C. For long-term storage, aliquot the reconstituted protein into single-use volumes and store at -20°C or -80°C. Avoid repeated freeze-thaw cycles, which degrade protein integrity. Adding a carrier protein such as 0.1% BSA can help stabilize the protein during storage, unless it interferes with your assay.

Is Recombinant Human LANCL1 Protein suitable for clinical or diagnostic use?

No. Recombinant Human LANCL1 Protein (rp369008) is designated for research use only (RUO) and has not been validated or approved for clinical, diagnostic, therapeutic, or veterinary applications. It should be handled in accordance with institutional biosafety guidelines.

References

1. Sturla L, Fresia C, Guida L, et al. LANCL2 is necessary for abscisic acid binding and signaling in human granulocytes and in rat insulinoma cells. J Biol Chem. 2009;284(41):28045-28057. doi:10.1074/jbc.M109.035329
2. Leber A, Hontecillas R, Zoccoli-Rodriguez V, Bassaganya-Riera J. Activation of LANCL2 by BT-11 ameliorates IBD by supporting regulatory T cell stability through stabilization of PPAR-γ and inhibition of AhR expression. Inflamm Bowel Dis. 2019;25(5):919-932. doi:10.1093/ibd/izy400
3. Fresia C, Vigliarolo T, Guida L, et al. G-protein coupling and nuclear translocation of the human abscisic acid receptor LANCL2. Sci Rep. 2016;6:26658. doi:10.1038/srep26658

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.