Recombinant Human LAYN Protein: FAQs & Specs

Recombinant Human LAYN Protein is a research-grade recombinant protein corresponding to human layilin, a type-I transmembrane C-type lectin implicated in cell adhesion, hyaluronan binding, and immune cell regulation.

Product Name

Recombinant Human LAYN Protein

SKU

rp365318

Gene / Protein

LAYN (Layilin); UniProt Q6UXZ3

Protein Class

Type-I transmembrane C-type lectin

Primary Function

Hyaluronan binding, cell adhesion, immune cell regulation

Supplier Reference

absea #PP-884

What Is LAYN (Layilin) and Why Is It Studied?

LAYN encodes layilin, a type-I transmembrane protein belonging to the C-type lectin superfamily. Its extracellular domain contains a carbohydrate recognition domain (CRD) that preferentially binds hyaluronan, a major component of the extracellular matrix (ECM). Through this interaction, layilin participates in cell–ECM adhesion and is thought to modulate cytoskeletal organisation at the cell periphery.

More recently, single-cell RNA sequencing and immunological studies have identified LAYN as a marker associated with exhausted or regulatory T cells (Tregs) within the tumour microenvironment, making it an emerging target in cancer immunology and immuno-oncology research.

Product Specifications and Formulation

Recombinant Human LAYN Protein (SKU: rp365318) is manufactured under controlled conditions to deliver a research-grade reagent suitable for in vitro assays. Key specification details such as expression host, tag configuration, molecular weight, purity grade, and endotoxin level are provided on the product datasheet (supplier reference: absea #PP-884). Researchers are strongly advised to consult the current Certificate of Analysis (CoA) and datasheet for lot-specific values before experimental use.

Typical recombinant proteins of this class are produced in mammalian or insect cell systems to preserve post-translational modifications relevant to lectin-domain functionality, but users should verify the exact expression system from the product documentation.

Reconstitution, Storage, and Stability

Proper handling is critical to maintaining protein activity. Upon receipt, inspect the vial and store lyophilised protein according to the datasheet recommendation — typically at −20 °C or −80 °C. Avoid repeated freeze–thaw cycles, as these can cause protein aggregation and loss of biological activity.

For reconstitution, centrifuge the vial briefly before opening. Reconstitute in the buffer specified on the datasheet (commonly sterile PBS or water supplemented with a carrier protein such as BSA) to achieve the recommended working concentration. Allow the protein to dissolve gently — do not vortex. After reconstitution, aliquot for single use and store at −80 °C. Refer to the current datasheet for stability data and shelf-life information specific to lot rp365318.

Recommended Applications

Recombinant Human LAYN Protein is intended for in vitro research use only and is not approved for diagnostic or therapeutic applications. Typical research applications for recombinant layilin protein may include:

• ELISA and binding assays — as a capture or detection antigen to study hyaluronan–layilin interactions.
• Cell-based functional assays — to investigate LAYN's role in cell adhesion or immune cell function.
• Antibody validation — as a positive control antigen for anti-LAYN antibody screening and western blot validation.
• Protein–protein interaction studies — pull-down and co-immunoprecipitation experiments to identify LAYN-binding partners.

Users should validate the protein for each specific assay format and optimise conditions accordingly. Refer to the datasheet for validated application details.

Ordering and Technical Support

Recombinant Human LAYN Protein is available from Aladdin Scientific under SKU rp365318. To place an order, contact your regional Aladdin Scientific representative or use the online ordering portal. For bulk quantities or custom formulations, please enquire with the sales team.

For technical questions regarding reconstitution protocols, assay optimisation, or protein characterisation, please contact Aladdin Scientific technical support, referencing SKU rp365318 and supplier lot number where available. The product datasheet (absea #PP-884) contains additional technical details and should be reviewed prior to use.

Frequently asked questions

What is the biological function of the LAYN (layilin) protein?

Layilin is a type-I transmembrane C-type lectin that binds hyaluronan through its extracellular carbohydrate recognition domain, supporting cell adhesion to the extracellular matrix and modulating peripheral cytoskeletal organisation. Emerging research also implicates LAYN as a marker of exhausted or regulatory T cells in tumour microenvironments, positioning it as a potential target in cancer immunology.

How should I reconstitute and store Recombinant Human LAYN Protein (rp365318)?

Briefly centrifuge the vial before opening, then reconstitute the lyophilised protein in the buffer specified on the product datasheet — typically sterile PBS or water with a carrier protein — to the recommended concentration; do not vortex. After reconstitution, aliquot for single use and store at −80 °C to avoid activity loss from freeze–thaw cycles. Always consult the current datasheet and Certificate of Analysis for lot-specific storage and stability instructions.

What applications is Recombinant Human LAYN Protein validated for?

This recombinant protein is intended for in vitro research use only. Common applications include ELISA and binding assays, cell-based functional studies, antibody validation as a positive control antigen, and protein–protein interaction experiments such as pull-down or co-immunoprecipitation. Specific validated applications and optimised conditions are detailed in the product datasheet (absea #PP-884); users should verify suitability for each assay format.

What expression system is used to produce LAYN recombinant protein, and does it affect glycosylation?

The specific expression host for rp365318 is detailed in the product datasheet. C-type lectins such as layilin can be glycosylated, and the choice of expression system (mammalian, insect, or bacterial) influences the glycosylation pattern, which may in turn affect binding activity and assay performance. Researchers should consult the datasheet to confirm the expression system and consider its implications for their specific experimental context.

How do I order Recombinant Human LAYN Protein from Aladdin Scientific?

Recombinant Human LAYN Protein is listed under SKU rp365318 on the Aladdin Scientific platform. You can order through the online portal or by contacting your regional Aladdin Scientific sales representative. For bulk quantities, custom specifications, or pricing enquiries, reach out to the sales team directly. Technical support is available for questions about product specifications and experimental use.

References

1. Bono P, Rubin K, Higgins JM, Bhatt DL, et al. Layilin, a novel integral membrane protein, is a hyaluronan receptor. Molecular Biology of the Cell, 2001.
2. Zheng C, et al. Landscape of infiltrating T cells in liver cancer revealed by single-cell sequencing. Cell, 2017. (LAYN identified as Treg/exhausted T cell marker in tumour microenvironment.)
3. Absea Biotechnology. Recombinant Human LAYN Protein, Product Reference PP-884. Absea Biotechnology Ltd.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.