Recombinant Human LCA5L Protein: FAQs & Specifications

Recombinant Human LCA5L Protein is a research-grade recombinant protein derived from the human LCA5-like gene, offered by Aladdin Scientific (SKU: rp372968) to support studies of ciliary function, photoreceptor biology, and related cellular pathways.

Product Name

Recombinant Human LCA5L Protein

SKU

rp372968

Gene / Protein

LCA5L (LCA5-like protein), a ciliary protein involved in photoreceptor and cilia biology

Supplier Reference

absea #PP-6917

Product Type

Recombinant protein for research use only

Intended Use

In vitro research applications including functional assays, antibody validation, and biochemical studies

What Is LCA5L and Why Is It Studied?

LCA5L (LCA5-like protein) is a gene and its encoded protein that shares structural and functional homology with LCA5 (Lebercilin), a ciliary protein associated with Leber congenital amaurosis (LCA), a severe inherited retinal dystrophy. LCA5L localizes to cilia and is thought to play a role in ciliary assembly, maintenance, and intraflagellar transport processes.

Research into LCA5L is relevant to understanding ciliopathies — a broad class of disorders caused by defects in cilia structure or function — as well as photoreceptor biology. Studying LCA5L at the protein level can clarify its interactions with other ciliary components and its potential contribution to retinal disease pathways.

Product Specifications and Quality

Recombinant Human LCA5L Protein (SKU: rp372968) is produced as a recombinant protein intended for research use. Specific details regarding the expression host system (e.g., E. coli, insect, or mammalian cells), purity grade, molecular weight, tag information, and endotoxin levels are not fully enumerated in the current source material. Researchers are advised to consult the official Aladdin Scientific product datasheet for the most current and complete specifications before ordering.

As a general guideline for recombinant proteins of this class, researchers should verify purity (typically ≥85% by SDS-PAGE), endotoxin levels, and the presence or absence of affinity tags when designing experiments.

Storage, Reconstitution, and Handling

Proper storage and handling of recombinant proteins are critical for maintaining activity and preventing degradation. While the specific storage conditions for rp372968 should be confirmed on the product datasheet, general best practices for recombinant proteins include:

• Short-term storage: Store at 4°C if the protein will be used within 1–2 weeks of receipt.
• Long-term storage: Aliquot and store at −20°C or −80°C to avoid repeated freeze-thaw cycles that can reduce protein activity.
• Reconstitution: If supplied as lyophilized powder, reconstitute in sterile deionized water or the buffer recommended on the datasheet. Allow the vial to equilibrate to room temperature before opening.
• Carrier protein: Adding a carrier protein such as 0.1% BSA can help stabilize dilute protein solutions.

Always refer to the official product datasheet for lot-specific storage instructions.

Recommended Applications and Experimental Considerations

Recombinant Human LCA5L Protein is intended for in vitro research use and may be suitable for a range of biochemical and cell biology applications. Typical applications for recombinant ciliary proteins of this type include:

• Protein–protein interaction studies: Co-immunoprecipitation or pull-down assays to identify binding partners within ciliary complexes.
• Antibody validation: Use as a positive control antigen to verify antibody specificity in Western blot or ELISA workflows.
• Biochemical assays: Enzyme-linked immunosorbent assays (ELISA) and binding assays.
• Structural studies: Supporting X-ray crystallography or cryo-EM experiments where pure, recombinant protein is required.

Researchers should empirically optimize protein concentrations and assay conditions for each specific application. This product is not intended for diagnostic, therapeutic, or clinical use.

Ordering and Support

Recombinant Human LCA5L Protein is available from Aladdin Scientific under SKU rp372968. When placing an order, please reference the supplier catalog number absea #PP-6917 if cross-referencing with the originating supplier.

For technical questions regarding protein specifications, compatibility with specific assays, or bulk/custom quantity requests, contact Aladdin Scientific's technical support team. For questions about pricing, promotions, shipping, or returns, visit the Aladdin Scientific website or contact customer service directly.

Researchers are encouraged to download the latest product datasheet prior to use, as lot-specific information—including purity data, SDS-PAGE images, and activity data—may be updated between production lots.

Frequently asked questions

What is the LCA5L protein and what is its biological function?

LCA5L (LCA5-like protein) is a ciliary protein that shares homology with LCA5/Lebercilin, which is implicated in Leber congenital amaurosis, a hereditary retinal dystrophy. LCA5L is thought to participate in ciliary assembly and intraflagellar transport, making it relevant to ciliopathy and photoreceptor research. Detailed functional characterization is an active area of investigation.

What expression system is used to produce Recombinant Human LCA5L Protein (rp372968)?

The specific expression system (e.g., E. coli, baculovirus/insect cells, or mammalian HEK293 cells) for rp372968 is not detailed in the current source material. Please download the official Aladdin Scientific product datasheet for rp372968 or contact our technical support team to obtain lot-specific information including the host expression system, tag, and purity data.

How should Recombinant Human LCA5L Protein be stored to maintain stability?

As with most recombinant proteins, short-term storage at 4°C is appropriate if the protein will be used within one to two weeks. For long-term storage, aliquot the protein and keep it at −20°C or −80°C to prevent activity loss from repeated freeze-thaw cycles. Always consult the product datasheet for lot-specific storage buffer and temperature recommendations.

What applications is Recombinant Human LCA5L Protein validated for?

This recombinant protein is intended for in vitro research use and may be applied in protein–protein interaction studies, antibody validation (e.g., as a Western blot or ELISA control antigen), and biochemical binding assays relevant to ciliary biology. Validated application data should be confirmed on the current product datasheet, as tested applications may vary by lot.

How do I order Recombinant Human LCA5L Protein from Aladdin Scientific?

Recombinant Human LCA5L Protein can be ordered directly from Aladdin Scientific using SKU rp372968. Visit the Aladdin Scientific website to add the product to your cart, or contact our sales team for bulk pricing, custom quantities, or institutional purchase order arrangements. Reference supplier catalog number absea #PP-6917 when cross-referencing with the originating manufacturer.

References

1. den Hollander AI, Koenekoop RK, Mohamed MD, et al. Mutations in LCA5, encoding the ciliary protein lebercilin, cause Leber congenital amaurosis. Nature Genetics. 2007.
2. Absea Biotechnology product reference PP-6917, Recombinant Human LCA5L Protein.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.