Recombinant Human MECOM Protein: Research Guide & Protocol

Recombinant Human MECOM Protein is a research-grade recombinant transcription factor that enables scientists to investigate the oncogenic, hematopoietic, and developmental roles of the MDS1 and EVI1 complex locus (MECOM) in well-controlled in vitro and cell-based assay systems.

Product Name

Recombinant Human MECOM Protein

SKU

rp369519

Protein Origin

Human (Homo sapiens)

Protein Class

Recombinant transcription factor / zinc-finger nuclear protein

Gene Name

MECOM (also known as EVI1, MDS1-EVI1)

Primary Research Areas

Hematopoiesis, leukemia, myelodysplastic syndrome, transcriptional regulation

Supplier Reference

absea#PP-4987

Protein Background & Biological Significance

MECOM (MDS1 and EVI1 Complex Locus) encodes a set of nuclear zinc-finger transcription factors that play critical roles in hematopoietic stem cell self-renewal, embryonic development, and oncogenesis. The locus produces multiple isoforms, most notably EVI1 and MDS1-EVI1, which differ in their N-terminal domains and functional properties.

Overexpression or rearrangement of MECOM at chromosomal band 3q26 is a well-documented oncogenic event associated with acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and other hematological malignancies. EVI1, the dominant oncogenic isoform, represses TGF-β signaling and interacts with key chromatin-remodeling complexes, contributing to aberrant transcriptional programs that block myeloid differentiation.

Recombinant Human MECOM Protein (rp369519) allows researchers to study these mechanisms directly in biochemical assays, providing a consistent and well-characterized tool for protein–DNA interaction studies, protein–protein interaction analyses, and functional reconstitution experiments.

Handling, Reconstitution & Storage Protocol

Upon Receipt: Inspect the vial for integrity. Recombinant proteins are typically shipped on dry ice or with ice packs. Confirm the product is frozen or cold upon arrival and document the receipt temperature.

Reconstitution: If supplied as a lyophilized powder, briefly centrifuge the vial before opening to consolidate the material. Reconstitute in the buffer specified on the Certificate of Analysis (CoA) — typically sterile phosphate-buffered saline (PBS) containing a carrier protein (e.g., 0.1% BSA) to stabilize the protein. Gently pipette to mix; avoid vortexing, which can denature the protein.

Storage:
• Short-term (up to 1 month): Store at 4 °C in the reconstituted form.
• Long-term: Aliquot into single-use volumes and store at −20 °C or −80 °C. Avoid repeated freeze-thaw cycles, as these reduce biological activity.
• Working aliquots: Keep on ice during bench work. Minimize time at room temperature.

Important: Always consult the product-specific CoA for the exact buffer composition, protein concentration, and storage recommendations, as these may vary by lot.

Experimental Applications & Assay Guidance

Recombinant Human MECOM Protein is suitable for a range of biochemical and cell-based research applications. Representative uses include:

• Electrophoretic Mobility Shift Assay (EMSA): MECOM zinc-finger domains bind specific DNA sequences. EMSA can confirm DNA-binding activity and assess compound-mediated disruption of MECOM–DNA complexes.
• Co-immunoprecipitation (Co-IP) and Pull-down Assays: Use recombinant MECOM protein to validate protein–protein interactions with known partners such as CtBP, HDAC complexes, and RUNX1.
• Enzyme-Linked Immunosorbent Assay (ELISA): Useful as a standard or coating antigen in sandwich or competitive ELISA formats when developing or validating anti-MECOM antibodies.
• Western Blot Standard: Serve as a positive control or molecular weight marker to confirm antibody specificity in lysate-based detection.
• Functional/Transcriptional Assays: Incorporate into cell-free transcription systems or use as a reference when benchmarking MECOM-driven transcriptional repression of target genes such as GATA2 and IL-3.

Optimal working concentrations should be determined empirically for each assay format. Refer to published literature and the Aladdin Scientific technical support team for application-specific recommendations.

Quality, Purity & Regulatory Considerations

Purity Verification: Recombinant proteins from Aladdin Scientific are quality-tested prior to release. Purity is typically assessed by SDS-PAGE and/or HPLC. The specific purity value and analytical data for rp369519 are detailed in the product's Certificate of Analysis (CoA), available upon request or via your Aladdin account portal.

Biological Activity: Functional assay data, where available, are reported on the CoA. Researchers are encouraged to verify activity in their specific assay systems, as matrix effects and assay conditions can influence outcomes.

Endotoxin Testing: For cell-based assays, endotoxin levels are a critical quality parameter. Confirm endotoxin specifications on the CoA before use in mammalian cell cultures to avoid confounding inflammatory responses.

Research Use Only: Recombinant Human MECOM Protein (rp369519) is intended for research purposes only. It is not approved for diagnostic or therapeutic use in humans or animals.

Lot-to-Lot Consistency: Aladdin Scientific provides lot-specific CoAs. Researchers running longitudinal studies are advised to procure sufficient quantity from a single lot or to validate new lots against their established assay benchmarks.

Frequently asked questions

What is MECOM and why is it important in cancer research?

MECOM (MDS1 and EVI1 Complex Locus) encodes nuclear zinc-finger transcription factors critical for hematopoietic stem cell self-renewal. Its overexpression or chromosomal rearrangement at 3q26 is linked to acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), making it a key target in hematological oncology research.

How should I reconstitute and store Recombinant Human MECOM Protein (rp369519)?

If supplied lyophilized, centrifuge the vial briefly before opening, then reconstitute in the buffer specified on the CoA — typically PBS with 0.1% BSA. For long-term storage, aliquot and store at −20 °C or −80 °C, avoiding repeated freeze-thaw cycles. Always consult the lot-specific CoA for exact instructions.

What experimental applications is Recombinant Human MECOM Protein suitable for?

Recombinant Human MECOM Protein is suitable for EMSA (DNA-binding studies), co-immunoprecipitation and pull-down assays, Western blot as a positive control or standard, ELISA antigen coating, and cell-free transcriptional assays. Optimal concentrations should be determined empirically for each application.

Is Recombinant Human MECOM Protein (rp369519) suitable for use in cell culture?

Before using in mammalian cell culture, verify the endotoxin level stated on the product CoA, as elevated endotoxin can trigger non-specific inflammatory responses that confound results. The protein is for research use only and is not intended for therapeutic or diagnostic applications.

How do I confirm the biological activity of the protein upon receipt?

Refer to the lot-specific CoA for any functional assay data provided by Aladdin Scientific. Additionally, running an EMSA with a known MECOM consensus DNA sequence or a Western blot against a validated anti-MECOM antibody can confirm protein integrity and activity in your laboratory's conditions.

References

1. Goyama S, Mulloy JC. Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. Int J Hematol. 2011;94(2):126-133.
2. Lugthart S, et al. Clinical, molecular, and prognostic significance of WHO type inv(3)(q21q26.2)/t(3;3)(q21;q26.2) and various other 3q abnormalities in acute myeloid leukemia. J Clin Oncol. 2010;28(24):3890-3898.
3. Wieser R. The oncogene and developmental regulator EVI1: expression, biochemical properties, and biological functions. Gene. 2007;396(2):346-357.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.