Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Biological processes are complex and determined by networks of interactions, called interactome networks, which include protein-protein interactions. The protein interactome is elemental to cellular function, but our full understanding of it is incomplete. One assay that has helped us begin to ...
Coelenterazine 400a is a chemically modified analog of native coelenterazine, designed to emit at shorter wavelengths (~395–400 nm) for high-sensitivity bioluminescent applications. This article explores its structural characteristics, origins, specific utility in BRET² and Ca²⁺ assays, ...
Updated Aug 202512 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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