Dialysis utilizes a semipermeable membrane with a defined molecular weight cut-off (MWCO). Driven by concentration gradients in a large external volume, small molecules diffuse out of the sample to achieve desalting, removal of small-molecule inhibitors/dyes, and buffer exchange. This document ...
Because the dialysis membrane is composed of spongy cross-linked polymers, the molecular weight cutoff (MWCO) representing the pore size of the membrane is an indirect parameter to measure the membrane separation performance. More precisely, the molecular weight of the membrane can be defined as ...
Generally, the semi permeable membrane is made into a bag, the sample solution of biomacromolecule is put into the bag, and the dialysis bag is immersed in water or buffer solution. The biomacromolecule with large molecular weight in the sample solution is retained in the bag, while the salt and ...
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