Terrific Broth, Modified for Fermentation, non-animal (Powder)

Non-animal sourced Tryptone: Utilizes a plant-based tryptone manufactured by a controlled enzymatic hydrolysis of plant proteins containing a mixture of peptides and free amino acids which provide a source of organic nitrogen and growth factors.

Directions per Liter:

Dissolve 47.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Potassium phosphate dibasic	9.4
Potassium phosphate monobasic	2.2
Tryptone (Animal Protein Free)	12
Yeast Extract	24
	Total: 47.6g/Liter
	500g makes 10.5 liters

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.

Homogeneous: Proprietary blending technology ensures complete mixing of components.