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Tris-Buffered Saline Tween-20 (TBST)

Protocols

Tris-Buffered Saline Tween-20 (TBST)

· Reviewed · Published Jun 9, 2025 · Updated Jul 27, 2023 · 8 min read

Recipe

1.Dissolve the following in 800ml of distilled H2O

8.8 g of NaCl
0.2g of KCl
3g of Tris base

2.Add 500ul of Tween-20

3.Adjust the pH to 7.4

4.Add distilled H2O to 1L

5.Sterilize by filtration or autoclaving

Categories: Protocols

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Tris-Buffered Saline Tween-20 (TBST)" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/tris-buffered-saline-tween-20-tbst-en.html

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