Laboratory Protocols & SOPs Library

TTC Sabouraud's medium preparation experiment

· Published 24 déc. 2024 · Updated 9 juin 2025

Summary

For isolation of yeast-like bacteria in clinical specimens. This experiment was obtained from the laboratory instruction of Mudanjiang Medical College undergraduate 5-year testing program.

Operation method

TTC Sabouraud Weak's medium preparation experiment

Principle

For isolation of yeast-like bacteria from clinical specimens.

Move

Ingredients:

Glucose: 40 g

Peptone: 10 g

Agar: 15 g

Distilled water: 1000 ml

Chloramphenicol: 50 mg

1% TTC aqueous solution: 5 ml

Preparation: Mix and dissolve the above 5 ingredients, final PH5.6, autoclave (121℃ for 15min) and then add chloramphenicol and TTC solution, mix thoroughly, split the test tube, place the slant or pour the plate.

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Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "TTC Sabouraud's medium preparation experiment" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/eu_fr/faqs/ttc-sabourauds-medium-preparation-exper-en.html

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