RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC) , CAS No.2711762-70-6

CAS: 2711762-70-6 Cat. No.: F1455371 Formule: C₃₈H₄₄N₄O₈ Poids moléculaire: 684.79

QUALITÉ & PURETÉ BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Synonyms

Fluorescent Probe for Intracellular L Fe²⁺ Fluorescent Probe bile Fe²⁺ | Intracellular Labile Fe²⁺ Fluorescent Probe | Fe2+ indicator

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Vue d’ensemble

 Iron is the most abundant transition metal element in organisms and participates in a wide range of physiological processes. In recent years, labile intracellular iron has attracted extensive research attention. Labile iron primarily exists in two stable redox states: ferrous ion (Fe²⁺) and ferric ion (Fe³⁺). For researchers investigating the intracellular reductive environment, metal transporters and the aqueous solubility of Fe²⁺, characterizing the behavior of Fe²⁺ is more critical than studying Fe³⁺. As a novel fluorescent probe, RhoNox-4 enables fluorescence imaging of intracellular Fe²⁺ in live cells. The fluorescence intensity enhancement induced by the reaction between RhoNox-4 and Fe²⁺ is irreversible, which distinguishes it from calcium-sensing fluorescent probes such as Fluo-3 (Cat.No.: F131394) that support real-time monitoring of calcium ions.

Spécifications

Synonymes

Fluorescent Probe for Intracellular L Fe²⁺ Fluorescent Probe bile Fe²⁺ | Intracellular Labile Fe²⁺ Fluorescent Probe | Fe2+ indicator

Spécifications et pureté

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Stabilité et stockage

Store at -20℃ long term (12 months). Store in the dark.

Conditions de stockage de stockage

Protected from light,Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

Biological Stain, BioReagent, pour l'analyse par fluorescence, pour la microscopie

Pureté

≥95%(HPLC)

Product Line

Produits biologiques

Images

IF: RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371)

RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371) - Flow 

Flow cytometric histograms of Jurkat cells stained with FerroOrange. 

Black: untreated cells without probe (negative control); 

Blue: untreated cells with probe (basal Fe²⁺ level); 

Red: cells pretreated with 100 μM ferrous ammonium sulfate (FAS) (A112646) for 30 min before probe incubation (elevated Fe²⁺); 

Green: cells pretreated with 100 μM 2,2'-bipyridyl (BPY) (D108977) for 30 min before probe incubation (Fe²⁺ chelation).

Flow: RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371)

RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371) - IF 

Confocal fluorescence images of Jurkat cells stained with the Fe²⁺ probe FerroOrange (RhoNox-4). 

Left: untreated cells show weak red fluorescence, indicating endogenous Fe²⁺. 

Middle: after treatment with 100 μM ferrous ammonium sulfate (FAS) (A112646) for 30 min, the red fluorescence intensity increases significantly, reflecting elevated intracellular Fe²⁺. 

Right: addition of 100 μM 2,2'-bipyridyl (BPY) (D108977), a Fe²⁺ chelator, binds Fe²⁺ and prevents its reaction with FerroOrange, resulting in a marked decrease in red signal.

Noms et identifiants

Poids moléculaire

684.79

Propriétés chimiques et physiques

• Solubilité: Soluble in acetonitrile, methanol and dimethyl sulfoxide (DMSO).