Grp75 Antibody - Anticorps primaire, specific to Grp75, Rabbit IgG

Cat. No.: Ab106524

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Validated, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Lapin

Reactive species

conseil, Humain, Souris

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human Grp75 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000 IHC: 1/50-1/100

Protein Function

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Spécifications

Product Name

Grp75 Antibody - Anticorps primaire, specific to Grp75, Rabbit IgG

Synonymes

75 kDa glucose regulated protein antibody|75 kDa glucose-regulated protein antibody|CSA antibody|Glucose Regulated Protein antibody|Grp 75 antibody|GRP-75 antibody|GRP75 antibody|Heat shock 70 kDa protein 9 antibody|Heat shock 70kD protein 9 antibody|heat shock 70kDa protein 9 antibody|Heat shock 70kDa protein 9B antibody|Heat shock protein A antibody|Hsc74 antibody|Hsp74 antibody|Hsp74a antibody|HSPA9 antibody|Hspa9a antibody|HSPA9B antibody|MGC4500 antibody|mitochondrial antibody|Mortalin 2 antibody|Mortalin antibody|Mortalin perinuclear antibody|Mortalin2 antibody|MOT 2 antibody|MOT antibody|MOT2 antibody|Mthsp70 antibody|P66 MOT antibody|PBP74 antibody|Peptide-binding protein 74 antibody|Stress-70 protein antibody|75 kDa glucose-regulated protein|CSA|GRP75|GRP-75|GRP75mthsp75|Heat shock 70 kDa protein 9|heat shock 70kD protein 9B|heat shock 70kDa protein 9 (mortalin)|heat shock 70kDa protein 9B (mortalin-2)|HSPA9|HSPA9B|HSPA9BMGC4500|Mortalin|mortalin, perinuclear|Mortalin-2|MOT|MOT2|mot-2|p66-mortalin|PBP74|Peptide-binding protein 74|stress-70 protein, mitochondrial|SAAN|CRP40|EVPLS|SIDBA4

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 0.6 mg/mL

Espèce hôte

Lapin

Spécificité

Grp75

Immunogène

A synthetic peptide derived from human HSPA9 (AA 114-157).

Contrôle positif

WB: A549, MCF-7, HEK293, HeLa, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

0.6 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - Western Blot
All lanes: Grp75 Antibody (Ab106524) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 75 kDa
Observed band size: 74 kDa
Exposure time: 4.5 s

IHC: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using Grp75 Antibody (Ab106524). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Grp75 Antibody (Ab106524)

Grp75 Antibody (Ab106524) - IF/ICC

IF analysis of Grp75 Antibody (Ab106524) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Grp75 Antibody (Ab106524) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

IF/ICC

1/200

Cibles associées (humaines)

HSPA9 Tchem Stress-70 protein, mitochondrial (0 Activities)

Discover Target: HSPA9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Grp75 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.