HAEGTFTSDVS - ≥98% , CAS No.864915-61-7

CAS: 864915-61-7 Cat. No.: H649167 Formule: C₄₈H₇₁N₁₃O₂₀ Poids moléculaire: 1150.15

QUALITÉ & PURETÉ ≥98%

Storage

Protected from light,Desiccated,Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

HAEGTFTSDVS is the first N-terminal 1-11 residues of GLP-1 peptide.

In Vitro

The GLP-1 (7-36) amide is a product of the preproGlukagon gene, which is secreted from intestinal L-cells, in response to the ingestion of food. GLP-1 exerts multiple actions by stimulating insulin secretion from pancreatic β-cells, in a glucose dependent manner (insulinotropic action). GLP-1 lowers circulating plasma Glukagon concentration, by inhibiting its secretion (production) from α-cells. GLP-1 also exhibits properties like stimulation of β-cell growth, appetite suppression, delays gastric emptying and stimulation of insulin sensitivity. The peptide analogs of GLP-1 are useful as Glukagon-like peptide 1 receptor agonists. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Form:Solid

Spécifications

Spécifications et pureté

≥98%

Mécanismes biochimiques et physiologiques

HAEGTFTSDVS is the first N-terminal 1-11 residues of GLP-1 peptide.

Conditions de stockage de stockage

Protected from light,Desiccated,Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Pureté

≥98%

Noms et identifiants

Poids moléculaire

1150.15

Application Protocols

No validated, item-specific application protocols are provided in the Product Data.

General handling suggestions (for planning only; verify in your lab context):

• Stock preparation: Dissolve the required mass in water or appropriate buffer to 1–10 mg/mL. If insolubility is observed, add a minimal volume of DMSO, then dilute with buffer.
• Aliquoting: Dispense into single‑use aliquots (e.g., 10–50 µL) in low‑bind tubes. Snap‑freeze in liquid nitrogen or dry‑ice/ethanol bath before transferring to −80 °C.
• Thaw/use: Thaw on ice; mix gently. Avoid foaming. Do not vortex vigorously if foaming/adsorption is a concern.
• QC check: Verify identity/purity by RP‑HPLC and MS when critical to your workflow. Determine concentration by A205/A214 or amino acid analysis if high accuracy is required.

These suggestions are general good‑practice notes and are not specifications or validated methods for this specific SKU.

Biological Roles

No biological/clinical claims are made for this product; research use only.

Literature context for the sequence:

• The peptide sequence HAEGTFTSDVS corresponds to residues 7–17 of human glucagon‑like peptide‑1 (GLP‑1), a proglucagon‑derived incretin hormone. This fragment is often used as a sequence-defined standard or control in biochemical experiments focused on GLP‑1 processing, stability, or recognition elements.
• Structural/biochemical features: The fragment includes His at the N‑terminus and acidic residues (E, D) that influence charge state and potential interactions with metal ions (e.g., transient His coordination) and surfaces. The Phe residue contributes weak aromatic absorbance and potential hydrophobic interactions.
• Enzymatic susceptibility: Short GLP‑1 fragments can be substrates for general endo/exopeptidases in vitro; exact rates depend on sequence context and assay conditions (pH, ionic strength, protease content).

Use cases in biochemistry (general):

• Spike‑in standards for LC–MS calibration of peptide charge state distributions.
• Controls in antibody epitope mapping to test recognition of the GLP‑1 N‑terminal region.
• Model peptide for studying peptide–membrane or peptide–surfactant interactions under defined conditions.

All roles above are provided as literature background and may not reflect validated uses for this specific lot; verify suitability experimentally.

Buffer Applications

This product is a peptide, not a buffering reagent. Therefore, it is not typically used to establish or maintain pH like standard buffers (e.g., phosphate, Tris, HEPES).

Practical notes when preparing peptide solutions:

• Choose a compatible buffer for your assay (e.g., 10–50 mM phosphate, HEPES, or ammonium bicarbonate for MS workflows). Maintain ionic strength (50–150 mM NaCl) if nonspecific adsorption is observed.
• pH selection: Neutral to mildly basic pH (6.5–8.0) often aids solubility for acidic peptides like HAEGTFTSDVS while limiting hydrolysis/deamidation.
• Avoid prolonged storage in extreme pH (<3 or >9), which can accelerate backbone hydrolysis and side‑chain modifications (e.g., deamidation of acidic residues).

Item-specific buffer specs: Not specified for this item; refer to CoA/Spec Sheet for any recommended reconstitution medium if provided.

Green Alternatives

Given that this product is a peptide, the principal greening opportunity is solvent choice and waste minimization.

• Prefer aqueous systems: Use water or benign buffers (phosphate, HEPES) rather than fluorinated alcohols (HFIP/TFE) or high DMSO percentages, unless strictly required for solubility.
• Minimize organic cosolvents: If DMSO or acetonitrile is needed to initially dissolve the peptide, dilute promptly into aqueous media to reduce organic load.
• Right‑first‑time dissolution: Perform small‑scale solubility checks to avoid repeated preparations and solvent waste.
• Storage efficiency: Prepare concentrated aliquots to reduce thaw/refreeze cycles and discard volumes.

Comparison (general):

• Water/buffer: lowest hazard, readily treated in aqueous waste streams (subject to local rules).
• DMSO: effective cosolvent; moderate environmental impact; manageable with standard solvent waste.
• HFIP/TFE: effective for difficult peptides; higher toxicity and environmental persistence; reserve for last resort.

Note: No item-specific environmental data are provided. Implement institutional green chemistry practices (microscale preparations, minimal single‑use plastics, energy‑efficient −80 °C storage management).

Pharmaceutical Uses

No therapeutic or clinical claims are made for this product; for research use only.

Formulation/manufacturing context (general):

• Reference standard: Short, sequence-defined peptides like HAEGTFTSDVS may be employed as analytical reference standards or process controls in pharmaceutical R&D, particularly in impurity profiling, digestion mapping, or LC–MS system suitability related to GLP‑1–derived products.
• Excipient status: This peptide is not an excipient. No pharmacopeial monograph is implied.
• Stability considerations: For any regulated use, define storage, handling, and reconstitution in a method SOP; determine counterion content, residual solvents, and water content for accurate potency calculations.

Item-specific regulatory/grade status: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific specifications (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

Literature/computed properties for the unmodified peptide HAEGTFTSDVS (for planning only; verify against CoA/SDS for this lot):

• Average molecular weight: ~1150.17 g/mol (sum of residue masses + H2O)
• Net charge and pI (prediction): acidic peptide; expected pI in the ~4–6 range due to E and D residues (exact value depends on terminal states and solvent ionic strength).
• Solubility tendencies: typically soluble in water and aqueous buffers (pH ≥ 6) at mg/mL levels; may require brief ultrasonic agitation. Initial dissolution in small volume of water or dilute buffer is recommended; DMSO can assist if aggregation occurs.
• UV absorbance (literature): weak at 280 nm (no Trp/Tyr; one Phe). For quantitation, consider A205/A214 methods or amino acid analysis/gravimetry.
• Aggregation: low propensity expected (no hydrophobic clusters; no Cys). Nonetheless, high concentrations and low ionic strength can promote self-association in some peptides.

General notes for peptides:

• Melting/boiling points are typically not applicable (decompose before melting; non-volatile solids).
• Hygroscopicity: peptides can be moisture sensitive; handle quickly in dry atmosphere to maintain mass accuracy.
• Refractive index, density, logP: not typically defined for solid peptides; rely on solution-phase measurements if needed.

Quality and Grades

From Product Data:

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and guidance (general, for peptide reagents):

• Common peptide grades include crude, desalted, >80–98% HPLC purity, and special analytical grades. Higher HPLC purity reduces background in biochemical assays and LC–MS.
• Typical release testing for peptides includes RP‑HPLC purity profile, mass identity (ESI/MALDI), and sometimes water/solvent content by Karl Fischer/GC. For this specific item, consult the CoA for actual methods and acceptance criteria.
• Counterions and salt form: Peptides are often supplied as TFA salts or acetate salts, which can influence mass and solubility. The counterion for this item is not specified; check CoA if salt form matters to your application.
• Residual solvents and additives (e.g., TFA, scavengers): Not specified for this item; refer to CoA/Spec Sheet.
• Implications for use: For quantitative bioassays or as LC–MS standards, consider performing an orthogonal purity check (e.g., additional HPLC run) and use accurate weighing techniques (drying under vacuum if appropriate) to account for moisture/counterion content.

Reaction and Applications

Manufacturer Applications: Not specified; see general research uses below.

Research/application contexts (literature/general for this sequence class):

• Reference fragment: HAEGTFTSDVS corresponds to the GLP‑1(7–17) region; used as a sequence-defined reference in LC–MS method development, retention time alignment, and system suitability for peptide separations.
• Epitope/mapping studies: Useful as a defined peptide for antibody epitope mapping or peptide array controls targeting the GLP‑1 region (no claims of biological activity are made).
• Enzymology/proteomics: Can serve as a substrate or standard for peptidases specific to this sequence motif, or as a calibrant for mass spectrometry (monoisotopic/average masses predictable).
• Assay development: Employed as a negative/positive sequence control in receptor-binding assay development or as a spike‑in recovery standard to assess sample handling losses.

Practical notes:

• Prepare fresh stock solutions in water or buffer; aliquot and freeze to avoid multiple freeze–thaw cycles.
• For quantitative work, determine concentration by amino acid analysis, dry mass by microbalance after equilibration in a desiccator, or spectrophotometrically at 205/214 nm using empirically determined extinction.
• For surface-coupling (e.g., SPR), consider N‑terminal immobilization via His or use a linker if steric access is required.

No specific reaction chemistry is associated with this item beyond standard peptide handling and optional conjugation through amine/carboxyl groups (EDC/NHS, NHS‑ester labeling) or side-chain functionalities (His, Ser/Thr under appropriate conditions).

Reaction Conditions

No item-specific reaction conditions are provided.

General conditions for common manipulations of short peptides like HAEGTFTSDVS (literature/practice):

• Dissolution: Water or 10–50 mM phosphate/HEPES, pH 6.5–8.0; gentle vortex and brief sonication. Typical stock concentrations: 1–10 mg/mL. If needed, pre-wet with minimal DMSO before dilution into buffer.
• EDC/NHS carboxyl activation (for conjugation): 10–50 mM MES buffer, pH 5.0–6.0; add EDC (2–10 mM) and NHS (5–25 mM); react 15–60 min at RT; immediately couple to amine partner at pH 7.0–8.5. Quench with Tris or ethanolamine.
• NHS‑ester labeling (N‑terminus/His-accessible): 50–100 mM bicarbonate or phosphate buffer, pH 8.3–8.5; dye:peptide 2–10 equiv; 0.5–2 h at RT protected from light.
• Immobilization via amines: Use activated esters on solid supports (e.g., NHS‑activated agarose) in pH 7.5–8.5 buffers for 1–2 h.
• LC–MS method development: RP‑HPLC with C18, 0.1% formic acid in water/acetonitrile; gradient 5–40% ACN over 10–30 min at 0.2–1 mL/min (analytical scale). ESI+ mode commonly yields [M+H]+ and multiply charged ions.

Expected outcomes: Short peptides generally reach near‑quantitative conversions in labeling/conjugation under optimized stoichiometry; verify by HPLC/MS. Always confirm that chosen conditions are compatible with intended downstream assays.

Note: Parameters above are general guidance only and not product specifications.

Safety and Handling

Authoritative safety data must be taken from the SDS for this specific item/lot.

From Product Data:

• Storage Conditions: Store at −80 °C, desiccated, protected from light.
• Shipped In: Dry ice packs + cold packs.
• GHS/Signal Word/H‑statements/Pictograms: Not specified for this item; refer to SDS.

General laboratory safety guidance for peptides (literature/practice):

• Likely low volatility; exposure routes are primarily dermal or inhalation of dust/aerosol. Avoid generating dust.
• PPE: lab coat, nitrile gloves, safety glasses; use a fume hood or powder hood during weighing/reconstitution.
• First aid (general): If inhaled—move to fresh air; if on skin—wash with soap/water; if in eyes—rinse cautiously with water; if ingested—rinse mouth. Seek medical advice per SDS.
• Handling: Allow vial to warm in a desiccator to room temperature before opening to minimize moisture uptake. Use antistatic tools/spatulas.
• Incompatibilities: Strong oxidizers can degrade peptides; avoid extreme pH (≤2 or ≥10) and elevated temperatures that promote hydrolysis/deamidation.
• Light sensitivity: Aromatic residues and some side chains can photo-oxidize; protect solutions and solids from light as specified.
• Waste: Dispose according to institutional and local regulations for organic laboratory waste/biochemical reagents.

Solvent Selection

Compound type: small, polar peptide (11 aa) with acidic side chains (E, D) and one aromatic residue (F).

• Primary solvent: Water or aqueous buffers (e.g., 10–50 mM phosphate, HEPES) are typically suitable. Begin with ultrapure water, then adjust pH/ionic strength as needed.
• pH considerations: Solubility generally improves above the pKa of acidic residues; neutral to slightly basic buffers (pH 6.5–8) commonly work well. Avoid prolonged exposure to high pH (>9) to limit base-catalyzed hydrolysis.
• Cosolvents: If needed, minimal DMSO (≤10% v/v) or acetonitrile (≤20% v/v) can help disrupt transient aggregation during stock preparation. Remove organic content if incompatible with downstream assays.
• Salt/ionic strength: 50–150 mM NaCl can reduce electrostatic self-association in some peptides.
• Filtration: Use low‑protein‑binding membranes (PTFE for organic mixes; PVDF/RC/nylon for aqueous) at ≥0.22 µm if sterile solutions are required.

Comparison (general):

• Water/buffer: green, compatible with bioassays; best first choice.
• DMSO: maximizes solubilization for hydrophobic peptides; here likely unnecessary but acceptable for concentrated stocks.
• HFIP/TFE: strong helix-promoters often used for difficult peptides; generally not needed for this sequence and less desirable environmentally.

Note: No item-specific solubility specifications are provided; validate solvent choice with a small test dissolve.

Storage and Reconstitution

From Product Data (authoritative for this item):

• Storage Conditions: Store at −80 °C, desiccated, protected from light.
• Shipped In: Dry ice packs + Cold packs.

Additional general guidance for peptides (not product specifications):

• Upon receipt: Keep frozen. Allow vial to equilibrate in a desiccator to room temperature before opening to prevent condensation and moisture uptake.
• Reconstitution: Use sterile, peptide‑compatible solvent (water or buffer). If needed, add a minimal amount of DMSO to initiate dissolution, then dilute with buffer. Filter through 0.22 µm low‑binding membrane if sterility is required.
• Aliquoting: Prepare single‑use aliquots to avoid repeated freeze–thaw cycles.
• Storage of solutions: Store working solutions at 2–8 °C for short periods (hours to ≤24 h). For longer storage, freeze at −80 °C. Avoid multiple freeze–thaw cycles.
• Stability: Light and moisture can accelerate degradation; maintain protection from light and keep desiccated for solid state stability.

Any specific concentration limits, pH recommendations, or shelf‑life claims are Not specified for this item; refer to CoA/Spec Sheet and SDS for lot‑specific guidance.

Structure and Identity

Short description: HAEGTFTSDVS is an 11–amino acid peptide (sequence: H-Ala-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser; N‑terminal His) corresponding to the GLP‑1(7–17) region (literature context). It is supplied as a research reagent (small-molecule/compound library category entry).

• SKU: H649167
• Product Name: HAEGTFTSDVS (peptide sequence)
• CAS: 864915-61-7
• Category Path: 全部 / 可售 / 小分子和化合物库
• Research Use: For research use only

Identity specifics from Product Data:

• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Literature/computed (for the unmodified free peptide HAEGTFTSDVS):

• Sequence (one-letter): HAEGTFTSDVS (11 aa)
• Residue composition: H(1), A(1), E(1), G(1), T(2), F(1), S(2), D(1), V(1)
• Average molecular weight (calc., free termini): ~1150.17 g/mol
• Structural features: linear L-peptide, polar/acidic character (E, D), one aromatic residue (F), no Cys or Met; free N-terminus (His) and free C-terminus (–COOH) assumed unless otherwise stated.
• 2D description in words: a linear chain of 11 α-amino acid residues linked by trans‑amide peptide bonds; side chains include imidazole (His), methyl (Ala, Val), carboxylate (Glu, Asp), hydroxyl (Ser, Thr), phenyl (Phe), and small hydrogen-only (Gly).

Synthetic Utility

As a defined 11‑mer peptide, HAEGTFTSDVS can serve as:

• SPPS intermediate/benchmark: A target for validating solid‑phase peptide synthesis (SPPS) workflows (Fmoc/tBu or Boc/benzyl) and HPLC purification strategies for short acidic peptides.
• Conjugation substrate: Contains functional handles for derivatization—N‑terminal amine (His), side-chain functionalities (His imidazole, Ser/Thr hydroxyls, Asp/Glu carboxylates), and C‑terminal carboxyl group—enabling bioconjugation (e.g., NHS‑ester dye labeling, EDC/NHS coupling to amines, click via installed azide/alkyne handles after selective modification).
• Ligation model: Useful in testing fragment condensation strategies (native chemical ligation analogs with auxiliary chemistry, oxime/hydrazone formation from selectively modified termini) though the native sequence lacks Cys.
• Analytical method development: Enables calibration of RP‑HPLC gradients, evaluation of ion‑pairing reagents, and optimization of MS ionization parameters for small peptides lacking strong 280 nm chromophores.

General cautions:

• Aromatic Phe allows limited UV tracking; consider A214/A205 or derivatization for stronger detection.
• Protect side chains appropriately during synthesis (tBu for Ser/Thr; Trt for His in Fmoc SPPS) and ensure thorough TFA scavenging to minimize adducts in the final material.
• Salt form/counterion control is important for reproducible chromatographic behavior.

Target Specificity

This product is a synthetic peptide, not an antibody or affinity reagent.

• Target/epitope specificity: Not applicable.
• Clone/isotype/species reactivity: Not applicable.

If using this peptide in binding assays (e.g., as a control ligand or mapping probe), target specificity must be established empirically for the intended system. No target-binding properties are claimed for this item.

Foire aux questions

What is the purity of this product?

This product is supplied at ≥98% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?80 °C, protected from light, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 864915-61-7, the molecular formula is C48H71N13O20, and the molecular weight is 1150.15 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.