Human Complement Decay-accelerating Factor (DAF/CD55) ELISA Kit

Cat. No.: EJ1515193

GRADE & PURITY BioReagent

Overview

This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human DAF;CD55. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human DAF;CD55 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.

Specifications

Specifications & Purity

BioReagent

Grade

BioReagent

Application

ELISA

Sample type

cell culture supernatants, Cell Lysate, Plasma, Serum, tissue homogenates

Detection Range

0.156ng/mL~10ng/mL

Detected Species Object

Human

Specificity

Detecting Human DAF/CD55 in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months.