Immunostaining Secondary Antibody Dilution Buffer - BioReagent, sterile-filtered, ready-to-use, Suitable for Immunohistochemistry, Suitable for Immunofluorescence

Cat. No.: I752114

QUALITÉ & PURETÉ Sterile-filtered, Suitable for Immunofluorescence(IF), BioReagent, Ready-to-use, Suitable for Immunohistochemistry(IHC)

Storage

Store at 2-8°C

Shipped In

Wet ice

Vue d’ensemble

This product is an optimized antibody diluent for immunostaining on the basis of traditional second antibody diluent, which can be used for the dilution of second antibody in immunostaining experiments such as immunohistochemistry and immunocytochemistry.
Operation process:
1. Reagent preparation refers to the instructions for the use of the second antibody used, as well as the quality of the primary antibody and the content of the target protein in the sample, diluting the second antibody in accordance with the appropriate proportion, such as 1:1000, 1:500 and other proportions.
2. Antibody incubation The second antibody can be incubated at the appropriate temperature after dilution of this product and directly used for immunostaining.
3, color reaction according to the needs of the experiment, choose the appropriate color reagent for color development.
Note: 
1. This product has been filtered treatment, once started, it is recommended to use within one month, if found precipitate or bacterial contamination please discard. 
2. The second antibody diluted by this product can be reused 3 to 5 times within 1 to 2 weeks under appropriate storage conditions. Tips For your own safety, before using the reagent, please take good protection, such as wearing lab clothes, gloves, etc.

Spécifications

Spécifications et pureté

BioReagent, sterile-filtered, ready-to-use, Suitable for Immunohistochemistry, Suitable for Immunofluorescence

Stabilité et stockage

Store at 2-8℃ for 12 months. Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

BioReagent, Filtré de manière stérile, Prêt à l'emploi, Suitable for Immunofluorescence(IF), Suitable for Immunohistochemistry(IHC)

Product Line

Produits biologiques

Foire aux questions

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Suitable for Immunofluorescence(IF) mean?

Suitable for Immunofluorescence(IF) indicates the product has been validated for use in immunoassays such as ELISA, IF, IHC and flow cytometry. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.