Eosin Methylene Blue Agar (Powder)

Directions per Liter:

Dissolve 36 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Pancreatic Digest of Gelatin	10
Lactose	5
Dipotassium Phosphate	2
Sucrose	5
Eosin Y	0.4
Methylene Blue	0.065
Agar	13.5
	Total: 36g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.