RSPH1 Mouse mAb: FAQs for Research Use

RSPH1 Mouse mAb is a mouse-derived monoclonal antibody targeting the RSPH1 protein, formulated in PBS and available from Aladdin Scientific (SKU: Ab351063) for research applications.

Product Name

RSPH1 Mouse mAb

SKU

Ab351063

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Formulation

PBS only

Target Protein

RSPH1 (Radial Spoke Head Protein 1)

What Is RSPH1 and Why Is It Studied?

RSPH1 (Radial Spoke Head Protein 1) is a structural component of the axonemal radial spoke, a multiprotein complex found in motile cilia and flagella. The radial spoke head plays a critical role in regulating ciliary beat pattern and motility. Mutations in RSPH1 have been associated with primary ciliary dyskinesia (PCD), a hereditary disorder characterized by defective ciliary motion, chronic respiratory disease, and laterality defects. Research into RSPH1 helps scientists understand the molecular basis of ciliopathies and normal ciliary function. A specific, validated monoclonal antibody such as RSPH1 Mouse mAb (Ab351063) is an important tool for detecting and localizing RSPH1 in experimental models.

Product Specifications and Formulation

The RSPH1 Mouse mAb (Ab351063) offered by Aladdin Scientific is produced in mouse and belongs to the monoclonal antibody class, ensuring high specificity and batch-to-batch consistency. The antibody is supplied in a PBS-only formulation, meaning it does not contain preservatives such as sodium azide or carrier proteins such as BSA unless otherwise stated. Researchers should be aware of this when planning downstream applications that may be sensitive to such additives. Key specifications include:

• SKU: Ab351063
• Host Species: Mouse
• Antibody Format: Monoclonal Antibody (mAb)
• Formulation: PBS only
• Supplier Reference: absea#KC-28458

For detailed concentration, clone number, isotype, and validated application data, please consult the official product datasheet available on the Aladdin Scientific website or contact technical support.

Storage, Handling, and Stability

Proper storage of RSPH1 Mouse mAb is essential to maintain antibody activity and prevent degradation. As a general guideline for PBS-formulated monoclonal antibodies:

• Store at 4 °C for short-term use (up to several weeks).
• For long-term storage, aliquot and freeze at −20 °C or −80 °C to avoid repeated freeze-thaw cycles.
• Avoid repeated freeze-thaw cycles, as this can lead to aggregation and loss of binding activity.
• Keep away from direct light and do not store in a frost-free freezer.

Always refer to the product-specific datasheet for validated storage conditions and expiry information, as recommendations may differ from general guidelines.

Recommended Applications and Usage Tips

Monoclonal antibodies targeting RSPH1 are commonly employed in a range of immunological and cell biology techniques. While specific validated applications for Ab351063 should be confirmed in the official datasheet, typical uses for anti-RSPH1 mAbs include:

• Western Blot (WB): Detection of RSPH1 protein in cell or tissue lysates.
• Immunofluorescence (IF): Localization of RSPH1 within ciliated cells and tissues.
• Immunohistochemistry (IHC): Assessment of RSPH1 expression in tissue sections.
• Immunoprecipitation (IP): Pull-down of RSPH1-containing protein complexes.

Users should optimize primary antibody dilutions, blocking conditions, and secondary antibody selection for their specific experimental system. The PBS-only formulation makes this antibody compatible with most standard laboratory protocols without the need to pre-dilute or remove carrier proteins.

Ordering and Customer Support

RSPH1 Mouse mAb is available from Aladdin Scientific under SKU Ab351063. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative. For technical questions regarding the antibody's performance, protocol optimization, or compatibility with specific assay systems, reach out to Aladdin Scientific's technical support team. When contacting support, please have the SKU (Ab351063) and lot number ready to ensure accurate and efficient assistance. Bulk quantities, custom formulations, or special packaging may be available upon request.

Frequently asked questions

What is the formulation of RSPH1 Mouse mAb (Ab351063)?

RSPH1 Mouse mAb (Ab351063) is formulated in PBS only. It does not contain preservatives or carrier proteins unless explicitly stated on the datasheet. Researchers should confirm the exact formulation details by reviewing the official product datasheet before use.

What applications is RSPH1 Mouse mAb recommended for?

Anti-RSPH1 monoclonal antibodies are commonly used in Western Blot, Immunofluorescence, Immunohistochemistry, and Immunoprecipitation. Specific validated applications for Ab351063 should be confirmed in the official product datasheet provided by Aladdin Scientific.

How should RSPH1 Mouse mAb be stored to maintain activity?

For short-term use, store the antibody at 4 °C. For long-term storage, aliquot the antibody and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles. Always refer to the product datasheet for specific storage recommendations.

What is RSPH1 and what is its biological significance?

RSPH1, or Radial Spoke Head Protein 1, is a key structural component of the axonemal radial spoke in motile cilia and flagella. It plays an important role in regulating ciliary beat pattern and motility. Mutations in RSPH1 have been linked to primary ciliary dyskinesia (PCD), making it a relevant target for research into ciliopathies and respiratory disease.

How do I order RSPH1 Mouse mAb from Aladdin Scientific?

You can order RSPH1 Mouse mAb using SKU Ab351063 directly through the Aladdin Scientific website or by contacting your regional sales representative. For bulk orders or custom packaging inquiries, please reach out to the Aladdin Scientific sales team directly.

References

1. Knowles MR, et al. Mutations in RSPH1 cause primary ciliary dyskinesia with a unique clinical and ciliary phenotype. Am J Respir Crit Care Med. 2014.
2. Aladdin Scientific. RSPH1 Mouse mAb, SKU Ab351063. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.