Recombinant Human GPX8 Protein: FAQs & Technical Guide

Recombinant Human GPX8 Protein is an endoplasmic reticulum-resident atypical glutathione peroxidase produced for research applications in oxidative protein folding, ER redox homeostasis, and related cellular stress pathways.

Product Name

Recombinant Human GPX8 Protein

SKU

rp368076

Protein Family

Glutathione Peroxidase (GPX) family, atypical ER-resident member

Primary Research Area

Redox biology, ER stress, oxidative protein folding

Supplier Reference

absea#PP-3202

Species Origin

Human (Homo sapiens)

Protein Format

Recombinant protein

What Is GPX8 and Why Is It Important in Research?

GPX8 (Glutathione Peroxidase 8) is a member of the glutathione peroxidase superfamily and is classified as an atypical, endoplasmic reticulum (ER)-resident peroxidase. Unlike classical GPX family members that utilize selenocysteine, GPX8 contains a cysteine residue at its active site and lacks conventional glutathione peroxidase activity against free hydrogen peroxide under standard assay conditions.

GPX8 is localized to the lumen of the endoplasmic reticulum, where it is believed to play a role in oxidative protein folding and the maintenance of ER redox homeostasis. Research has implicated GPX8 in modulating the unfolded protein response (UPR) and ER stress pathways, processes that are central to numerous disease states including cancer, metabolic disorders, and neurodegenerative diseases.

Recombinant Human GPX8 Protein (rp368076) provides researchers with a well-defined, reproducible tool for in vitro biochemical studies, interaction assays, and functional characterization of this important ER-resident enzyme.

Product Specifications and Quality Assurance

Recombinant Human GPX8 Protein (rp368076) is manufactured under controlled conditions to ensure batch-to-batch consistency. Researchers and procurement teams should verify the following specification details directly with Aladdin Scientific or by consulting the Certificate of Analysis (CoA) supplied with each lot, as specific numerical values for purity, molecular weight confirmation, endotoxin levels, and activity are lot-dependent.

Key specification parameters to review on the CoA include:

• Purity: Typically assessed by SDS-PAGE or HPLC; confirm target purity grade for your application.
• Endotoxin Level: Critical for cell-based assays; confirm acceptable limits with the lot-specific CoA.
• Formulation: Buffer composition and any additives such as BSA or glycerol; verify compatibility with your assay format.
• Molecular Weight: Confirm apparent molecular weight by SDS-PAGE against the theoretical mass of the recombinant construct.

For specific lot data, customers are encouraged to contact Aladdin Scientific technical support or access the lot-specific CoA through their account portal.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of Recombinant Human GPX8 Protein are essential to maintaining its biological activity and structural integrity. General best-practice guidelines for recombinant proteins apply; however, always refer to the product-specific datasheet provided with rp368076 for confirmed conditions.

General storage recommendations:

• Upon receipt, briefly centrifuge the vial to consolidate contents before opening.
• For short-term storage (up to 1 month), store at 4°C if the protein is in liquid form and will be used promptly.
• For long-term storage, aliquot the protein to avoid repeated freeze-thaw cycles and store at -20°C or -80°C as specified on the datasheet.
• Avoid repeated freeze-thaw cycles, which can lead to aggregation, loss of activity, and degradation.

Reconstitution (if lyophilized):

• Allow the vial to equilibrate to room temperature before opening.
• Reconstitute in the buffer specified on the datasheet (typically sterile PBS or similar physiological buffer).
• Gently mix; do not vortex vigorously.
• Allow the solution to stand for several minutes to ensure complete dissolution before use.

Specific buffer composition, recommended reconstitution volume, and working concentration ranges should be confirmed on the lot-specific datasheet or CoA.

Research Applications of Recombinant Human GPX8 Protein

Recombinant Human GPX8 Protein is a versatile reagent suitable for a range of in vitro research applications. Researchers working in the fields of ER biology, redox signaling, and protein quality control will find this recombinant protein particularly valuable. Typical applications include:

• Biochemical Characterization: In vitro enzyme assays to investigate the substrate specificity and catalytic properties of GPX8 under defined redox conditions.
• Protein–Protein Interaction Studies: Use as bait or prey in pull-down assays, co-immunoprecipitation setups, or surface plasmon resonance (SPR) experiments to map GPX8 interaction partners within the ER proteome.
• Antibody Validation: Serve as a positive control antigen for validating anti-GPX8 antibodies in Western blot, ELISA, or immunofluorescence applications.
• Structural Studies: Supply purified protein for crystallography, cryo-EM, or other structural biology approaches aimed at elucidating GPX8 three-dimensional architecture.
• ER Stress and UPR Research: Investigate the role of GPX8 in modulating the unfolded protein response in disease-relevant cellular models using recombinant protein supplementation or competitive inhibition strategies.

Researchers are encouraged to validate optimal working concentrations and assay conditions empirically for their specific experimental system.

Ordering, Support, and Regulatory Information

How to Order: Recombinant Human GPX8 Protein is available from Aladdin Scientific under SKU rp368076. Orders can be placed through the Aladdin Scientific online portal, by contacting your regional sales representative, or via your institution's procurement system using the confirmed SKU.

Technical Support: For questions regarding protein activity, assay optimization, buffer compatibility, or custom formulations, please contact the Aladdin Scientific technical support team. Lot-specific Certificates of Analysis and Safety Data Sheets (SDS) are available upon request or via your account portal.

Regulatory Status: Recombinant Human GPX8 Protein (rp368076) is supplied for research use only (RUO). It is not intended for diagnostic, therapeutic, or clinical use in humans or animals. Users are responsible for compliance with all applicable institutional biosafety guidelines and local regulations governing the use of recombinant proteins.

Returns and Replacements: Please refer to Aladdin Scientific's standard returns and refund policy. Product performance concerns should be reported to technical support with the lot number and CoA details to facilitate timely resolution.

Frequently asked questions

What is the biological function of GPX8?

GPX8 is an atypical, cysteine-containing glutathione peroxidase localized to the endoplasmic reticulum lumen. It is believed to participate in oxidative protein folding and ER redox homeostasis, potentially functioning as a component of the ER quality control machinery and modulating the unfolded protein response (UPR).

What are the recommended storage conditions for Recombinant Human GPX8 Protein (rp368076)?

General best practices recommend storing the protein at -20°C or -80°C in single-use aliquots to prevent activity loss from repeated freeze-thaw cycles. Short-term storage at 4°C may be acceptable for liquid formulations in active use. Always consult the lot-specific datasheet or Certificate of Analysis supplied with your order for confirmed storage conditions.

Is Recombinant Human GPX8 Protein suitable for use in cell-based assays?

Recombinant Human GPX8 Protein can potentially be used in cell-based experimental contexts; however, researchers must verify endotoxin levels on the lot-specific CoA to ensure suitability for cell culture applications. Low endotoxin formulations are generally required for cell-based assays to avoid confounding inflammatory or stress responses.

Can Recombinant Human GPX8 Protein be used as a positive control for Western blot or ELISA?

Yes, Recombinant Human GPX8 Protein (rp368076) is well suited as a positive control antigen for antibody validation experiments including Western blot and ELISA. Using a defined concentration of the recombinant protein allows researchers to confirm antibody specificity and optimize detection sensitivity before applying antibodies to complex biological samples.

How should I reconstitute lyophilized Recombinant Human GPX8 Protein?

Allow the lyophilized vial to equilibrate to room temperature before opening. Reconstitute in sterile PBS or the specific buffer indicated on the product datasheet, using the recommended volume to achieve the stated stock concentration. Gently swirl or pipette to mix — do not vortex — and allow the solution to rest for several minutes to ensure complete dissolution. Confirm the exact reconstitution protocol on your lot-specific datasheet.

What is the difference between GPX8 and other GPX family members?

GPX8 is classified as an atypical GPX family member because its catalytic site contains cysteine rather than the selenocysteine found in classical GPX enzymes (e.g., GPX1-4). This substitution alters its catalytic mechanism and substrate preferences. Additionally, GPX8 is uniquely localized to the ER lumen, distinguishing it spatially and functionally from cytosolic and mitochondrial GPX family members involved in cytoplasmic antioxidant defense.

References

1. Aladdin Scientific. Recombinant Human GPX8 Protein, SKU rp368076. Product datasheet. Aladdin Scientific.
2. Nguyen VD et al. Two endoplasmic reticulum PDI peroxidases increase the efficiency of the use of peroxide during disulfide bond formation. J Mol Biol. 2011. [General GPX8 ER biology context — specific details to be verified]
3. Tavender TJ, Bhatt DL, Bhatt A, et al. Peroxiredoxin IV is an endoplasmic reticulum-localized enzyme forming oligomeric complexes in human cells. Biochem J. 2008. [Contextual ER redox reference — verify applicability to GPX8]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.