Recombinant Human FHIP1B Protein: Research Guide & Applications

Recombinant Human FHIP1B Protein is a research-grade recombinant protein implicated in dynein-mediated endosomal cargo transport, offering a valuable tool for investigating intracellular trafficking pathways and FTS/Hook/FHIP complex biology.

Product Name

Recombinant Human FHIP1B Protein

SKU

rp372852

Protein Family

FTS and Hook Interacting Protein (FHIP) family

Biological Function

Involved in dynein–dynactin cargo adaptor complex assembly and endosomal trafficking

Species Origin

Human (Homo sapiens)

Recommended Applications

Protein interaction studies, functional assays, antibody validation, structural studies

Storage Recommendation

Avoid repeated freeze-thaw cycles; store in aliquots at recommended temperature

Biological Background of FHIP1B

FHIP1B (FTS and Hook Interacting Protein 1B), also referred to as FAM160A1 in some databases, is a member of the FHIP protein family. This family of proteins was identified through their shared ability to interact with Hook proteins and Fused Toes (FTS), forming the FTS–Hook–FHIP (FHF) complex.

The FHF complex functions as a cargo adaptor for the cytoplasmic dynein–dynactin motor machinery, which is responsible for minus-end-directed microtubule-based transport within the cell. By bridging specific membrane-bound organelles or vesicles to the dynein–dynactin complex, FHIP family members including FHIP1B play an essential role in the retrograde transport of endosomal cargo along microtubules.

Dysregulation of endosomal trafficking pathways has been associated with multiple disease states, making FHIP1B and its interaction partners of significant interest to researchers studying intracellular logistics, neurodegeneration, and vesicular sorting mechanisms.

Product Specifications and Formulation

Recombinant Human FHIP1B Protein (SKU: rp372852) is produced using recombinant expression technology to deliver a well-characterized research-grade reagent suitable for a range of biochemical and cell biology applications.

Key specification details—including exact molecular weight, expression host system (e.g., E. coli, baculovirus, or mammalian), tag information, purity grade, and buffer composition—should be confirmed from the official Aladdin Scientific product datasheet for SKU rp372852, as these parameters may vary by lot. Researchers are advised to download the current Certificate of Analysis (CoA) prior to experimental use.

General formulation guidance for recombinant proteins of this class includes: preparation in physiologically compatible buffer (such as PBS or HEPES-based formulations), supplementation with carrier protein (e.g., BSA) where applicable to prevent adsorptive loss, and quality assessment by SDS-PAGE and/or Western blot prior to use in sensitive assays.

Handling, Storage, and Stability Guidelines

Proper handling and storage of Recombinant Human FHIP1B Protein are critical to maintaining biological activity and experimental reproducibility. The following best-practice guidelines apply to recombinant proteins of this class:

• Upon receipt: Briefly centrifuge the vial to collect contents, then reconstitute or dilute according to the datasheet instructions.
• Short-term storage: If the protein will be used within 1–2 weeks, store at 4 °C in the supplied buffer. Avoid prolonged storage at 4 °C to prevent proteolytic degradation.
• Long-term storage: Aliquot into single-use volumes and store at −20 °C or −80 °C as specified on the product datasheet. Aliquoting prevents activity loss due to repeated freeze-thaw cycles.
• Freeze-thaw cycles: Limit to a maximum of 2–3 cycles. Each freeze-thaw event may reduce protein activity and increase aggregation risk.
• Working solutions: Prepare fresh dilutions on the day of the assay using a compatible, protein-stabilizing buffer.

Always refer to the lot-specific CoA for precise storage temperature and shelf-life data.

Research Applications

Recombinant Human FHIP1B Protein is suitable for use in a variety of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: As a component of the FHF complex, FHIP1B is well suited for co-immunoprecipitation (Co-IP), pull-down assays, and surface plasmon resonance (SPR) experiments aimed at characterizing its interactions with Hook proteins, FTS, dynein, and dynactin subunits.
• Biochemical reconstitution assays: Recombinant FHIP1B can be incorporated into in vitro reconstitution experiments to study FHF complex assembly and its effect on dynein processivity and cargo selectivity.
• Antibody validation: The purified protein serves as a positive control antigen for validating FHIP1B-specific antibodies by Western blot, ELISA, or dot blot.
• Structural biology: The recombinant protein may be used in crystallography or cryo-EM studies aimed at elucidating the structural basis of FHF complex formation and dynein regulation.
• Drug discovery screening: FHIP1B may serve as a target or tool protein in high-throughput screens designed to identify modulators of endosomal trafficking.

Frequently asked questions

What is FHIP1B and what is its role in the cell?

FHIP1B (FTS and Hook Interacting Protein 1B) is a member of the FHIP protein family that participates in the FTS–Hook–FHIP (FHF) complex. This complex acts as a cargo adaptor for the cytoplasmic dynein–dynactin motor, facilitating retrograde endosomal transport along microtubules. Disruption of FHIP1B function may impair intracellular trafficking fidelity.

What applications is Recombinant Human FHIP1B Protein (rp372852) suitable for?

The protein is suitable for protein–protein interaction studies (Co-IP, pull-down, SPR), in vitro biochemical reconstitution of the FHF complex, antibody validation by Western blot or ELISA, structural biology investigations, and use as a tool protein in drug discovery assays targeting endosomal trafficking pathways.

How should Recombinant Human FHIP1B Protein be stored to maintain activity?

For short-term use (1–2 weeks), store at 4 °C. For long-term storage, aliquot into single-use volumes and keep at −20 °C or −80 °C as directed on the product datasheet. Minimize freeze-thaw cycles to no more than 2–3 times to preserve protein integrity and activity. Always consult the lot-specific Certificate of Analysis for definitive storage conditions.

Where can I find the detailed specifications and Certificate of Analysis for rp372852?

Detailed specifications, including molecular weight, expression host, purity, tag information, and buffer composition, as well as the lot-specific Certificate of Analysis (CoA), are available on the Aladdin Scientific product page for SKU rp372852. Contact Aladdin Scientific technical support if you require additional documentation.

References

1. Guardia CM, De Pace R, Mattera R, Bonifacino JS. Neuronal functions of adaptor complexes involved in protein sorting. Current Opinion in Neurobiology. 2018;51:103–110.
2. Xie S, Farmer T, Bhatt D, et al. FHF complex-mediated dynein recruitment drives retrograde endosomal transport. Journal of Cell Biology. 2019.
3. Reck-Peterson SL, Redwine WB, Vale RD, Carter AP. The cytoplasmic dynein transport machinery and its many cargoes. Nature Reviews Molecular Cell Biology. 2018;19(6):382–398.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.