KCNC2 Antibody - Anticorps primaire, specific to 3747, Rabbit IgG

Cat. No.: Ab191501

GRADE & PURITY 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Lapin

Reactive species

Cependant, conseil, Humain, Pig, Souris

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human KCNC2 Antibody, Polyclonal, could be used for WB, IHC and so on.

Specifications

Product Name

KCNC2 Antibody - Anticorps primaire, specific to 3747, Rabbit IgG

Synonymes

Potassium voltage-gated channel subfamily C member 2 | Voltage-gated potassium channel Kv3.2 | KCNC2 | KV3.2 | DEE103

Spécifications et pureté

1.0 mg/mL

Mécanismes biochimiques et physiologiques

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain. Contributes to the regulation of the fast action potential repolarization and in sustained high-frequency firing in neurons of

Espèce hôte

Lapin

Spécificité

3747

Immunogène

A KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Kv3.2.

Contrôle positif

WB: MCF7 and HeLa cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Immunogen affinity purified

Concentration

1.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: KCNC2 Antibody (Ab191501)

KCNC2 Antibody (Ab191501) - Western Blot
All lanes: KCNC2 Antibody (Ab191501) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 70 kDa
Observed band size: 75 kDa
Exposure time: 100.4 s

IHC: KCNC2 Antibody (Ab191501)

KCNC2 Antibody (Ab191501) - IHC
Immunohistochemical analysis of KCNC2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with KCNC2 Antibody (Ab191501) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: KCNC2 Antibody (Ab191501)

KCNC2 Antibody (Ab191501) - IF/ICC
IF analysis of KCNC2 Antibody (Ab191501) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with KCNC2 Antibody (Ab191501) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

IF/ICC

1/200

WB

1/500-1/1000

IHC

1/50-1/100

Cibles associées (humaines)

KCNC2 Tclin Potassium voltage-gated channel subfamily C member 2 (0 Activities)

Discover Target: KCNC2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (KCNC2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.