Ly-6C Rat mAb - Anticorps primaire, specific to Ly6c, Rat IgG2c

Cat. No.: Ab113752

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Application

Flow Cytometry

Host species

conseil

Reactive species

Souris

Isotype

Rat IgG2c

Clone Number

HK1.4

Conjugation

Unconjugated

Vue d’ensemble

Rat anti Mouse Ly-6C Antibody, Monoclonal (HK1.4), could be used for Flow and so on.
Application:
Flow: 1.0 μg/100w cells

Spécifications

Product Name

Ly-6C Rat mAb - Anticorps primaire, specific to Ly6c, Rat IgG2c

Synonymes

Ly 6c antibody; Ly6c protein antibody; Ly6c1 antibody; Ly6c2 antibody; Lymphocyte antigen 6 complex antibody; Lymphocyte antigen 6 complex locus C antibody; Lymphocyte antigen 6C antibody; Lymphocyte antigen 6C1 antibody; Lymphocyte antigen 6C2 antibody;

Spécifications et pureté

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA

Espèce hôte

conseil

Spécificité

Ly6c

Immunogène

L3 cloned CTL cells

Contrôle positif

Flow: Murine splenocytes.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Rat IgG2c

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Stabilité et stockage

Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: Ly-6C Rat mAb (Ab113752)

Ly-6C Rat mAb (Ab113752) - Flow Cytometry
Two-color flow cytometric analysis of Ly-6C expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD8 alpha Rat mAb (APC) (Ab181178) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or Ly-6C Rat mAb (Ab113752; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD8 alpha versus Ly-6C (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Flow Cytometry: Ly-6C Rat mAb (Ab113752)

Ly-6C Rat mAb (Ab113752) - Flow Cytometry
Flow Cytometry analysis of mouse bone marrow cells labelling Ly-6C (red) with Ly-6C Rat mAb (Ab113752) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

1μg/100w cells

Cibles associées (humaines)

BIN1 Tbio Myc box-dependent-interacting protein 1 (0 Activities)

Discover Target: BIN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Ly-6C Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.