Meat Peptic Digest - BioReagent

Cat. No.: M1528326

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

Meat peptone digest is obtained by pepsin digestion of animal tissue. It is soluble in water, yielding a clear and transparent solution with no phosphate precipitation, and is used for the preparation of microbial culture media. Composition: Total nitrogen ≥ 12.8%, amino nitrogen ≥ 3.2%, ash ≤ 5.0%, moisture ≤ 5.0%

Specifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Product Line

Produits biologiques

Application Protocols

The following are general, literature-based examples for laboratory media preparation using Meat Peptic Digest. They are provided as non-specification guidance; optimize for your organism and workflow.

1. Nutrient Broth (general baseline medium):

• Meat Peptic Digest: 5.0 g/L
• Beef/Meat Extract: 3.0 g/L
• Sodium Chloride: 5.0 g/L
• Water to 1 L. Adjust pH to ~7.0. Autoclave at 121 C for 15–20 min.

2. Enriched Complex Broth (for robust biomass):

• Meat Peptic Digest: 10.0 g/L
• Yeast Extract: 5.0 g/L
• Glucose: 5.0–10.0 g/L
• NaCl: 5.0 g/L (optional, organism-dependent)
• Phosphate buffer (to target pH). Sterilize appropriately.

3. Filter-sterile supplement stock (10x):

• Dissolve Meat Peptic Digest at 100 g/L in warm DI water. Clarify through 0.45 µm then sterilize through 0.22 µm. Store 2–8 C for up to 1–2 weeks. Add aseptically to sterile base medium to achieve desired final concentration.

General steps:

• Dissolve components sequentially with moderate stirring.
• Adjust pH only after full dissolution.
• For plates, add 15 g/L agar before autoclaving.
• Validate with growth promotion tests using reference strains (e.g., E. coli, S. aureus, B. subtilis) and compare to internal controls.

Note: Concentrations and conditions are literature examples and not specifications for this item.

Biological Roles

Meat Peptic Digest functions in culture systems as a complex nutrient pool rather than exerting a specific biochemical effect. Its biological relevance is tied to the composition of peptides, amino acids, and micronutrients released by pepsin digestion of animal proteins (literature/general).

• Nitrogen source: Supplies readily assimilable peptides and free amino acids for microbial biosynthesis (protein, nucleotides, cell wall components).
• Growth factors: Contributes vitamins, minerals, and small molecules naturally present in muscle tissue that can stimulate growth of fastidious organisms.
• Peptide transport: Many bacteria preferentially import di-/tripeptides via peptide permeases; peptic digests provide substrates well-suited to these transport systems.
• Metabolic buffering: Peptides can modestly buffer pH changes via amino/carboxyl groups, though dedicated buffers typically set the medium pH.
• Impact on phenotype: The undefined composition can influence metabolite profiles, enzyme expression, and secretion titers; hence, lot qualification via growth promotion testing is recommended for critical applications.
• Host specificity: Supports a broad range of bacteria and some fungi/yeasts when combined with appropriate carbon sources and salts (organism-dependent, literature/general).

This product is intended strictly for research use. It is not a therapeutic, diagnostic, or clinical product.

Buffer Applications

This material is not a buffering reagent in the classical sense and has no defined pKa profile as a mixture. It does not serve as a primary buffer component.

Practical notes for media preparation (literature/general):

• pH setting: Adjust medium pH after all components (including Meat Peptic Digest) are dissolved. Use standard buffers (e.g., phosphate) or base/acid as needed to reach target pH for the organism.
• Ionic strength: Add NaCl or other salts per the chosen recipe (e.g., Nutrient Broth/Agar formulations).
• Sterilization: Autoclave complete media if compatible; for heat-sensitive supplements, sterilize the Meat Peptic Digest solution separately by filtration and combine aseptically.
• Clarity: If optical measurements are required (e.g., OD600), minimize turbidity by filtering the solution before use.

For true buffer applications, select defined buffering agents such as phosphate, Tris, HEPES, MOPS, or acetate tailored to the desired pH range.

Green Alternatives

Considerations for sustainability and regulatory preferences (literature/general):

• Animal-origin reduction: Replace meat-derived peptones with plant- or microbial-derived hydrolysates where feasible.

Comparison (general, non-specification):

| Option | Source | Advantages | Tradeoffs | |---|---|---|---| | Meat Peptic Digest | Animal tissue (meat), pepsin hydrolysis | Broad nutrient spectrum; strong growth performance for fastidious microbes | Animal-origin concerns (TSE/BSE, ethics), batch variability | | Soy/Plant Peptones | Soy, wheat, pea, potato | Animal-component–free; sustainable; good amino nitrogen; consistent supply | Different peptide profile; may require re-optimization; possible phytoinhibitors | | Yeast Extract + Plant Peptone | Microbial + plant | Rich in vitamins, nucleotides; strong growth factors | Cost; composition variability | | Chemically Defined Media (CDM) | Synthetic amino acids/salts | Highest reproducibility; regulatory clarity | Higher cost/complexity; organism-specific optimization required |

Implementation tips:

• Perform side-by-side growth curves when transitioning to animal-free media.
• Use a design-of-experiments approach to adjust total nitrogen, carbon sources, and trace elements.
• Obtain vendor statements on allergen status, GMO status, and residual solvent profiles for plant-derived alternatives.

Note: Choice depends on organism, regulatory constraints, and target product quality attributes.

Pharmaceutical Uses

No therapeutic or clinical use is implied. In pharmaceutical and biomanufacturing research contexts (literature/general):

• Fermentation media component: Utilized as a complex nitrogen and growth factor source in upstream development for microbial production of research-grade enzymes, metabolites, and recombinant proteins.
• Seed trains and inoculum preparation: Included in non-selective media to establish robust biomass prior to transfer into production media (where composition may be animal-component–free in later stages).
• Process development: Serves as a benchmark complex supplement when screening carbon/nitrogen ratios, feeding strategies, and DO/pH control parameters.
• Regulatory considerations: As an animal-derived material, sourcing documentation (e.g., TSE/BSE statements, country of origin) and risk assessments may be required in GMP environments. Such documentation is Not specified for this item; consult CoA/Spec Sheet or supplier.
• Downstream impact: Being undefined, it can introduce lot-to-lot variability in impurity profiles; defined or animal-free alternatives are often preferred for late-stage process lock.

Note: For any use associated with human or veterinary products, follow institutional policies and regulatory guidance. This item is for research use only.

Physical Properties

Because Meat Peptic Digest is an undefined peptide mixture, conventional single-compound properties do not apply. Where helpful, general/literature characteristics for peptone-type digests are provided.

Item-specific values:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not applicable as a single value; polydisperse peptide mixture.

General/literature properties (non-specification):

• Form: Typically a free-flowing powder or granulate (literature/general).
• Color/Odor: Light tan to brown; characteristic protein hydrolysate odor (literature/general).
• Solubility: Freely soluble in water; forms clear to slightly opalescent solutions depending on concentration and source (literature/general). Poorly soluble/insoluble in most organic solvents.
• Solution behavior: Aqueous solutions can exhibit light scattering due to colloidal components; filtration (0.22–0.45 µm) often clarifies (literature/general).
• pH of aqueous solution: Dependent on concentration and lot; pepsin digests can be slightly acidic prior to adjustment (literature/general). Not specified for this item.
• Hygroscopicity: Protein hydrolysates are moderately hygroscopic; protect from atmospheric moisture to prevent caking (literature/general).
• Thermal stability: Dry materials tolerate ambient temperatures; prolonged heating of solutions can cause Maillard darkening in presence of reducing sugars (literature/general).

Note: For media formulation, labs commonly prepare 5–20 g/L stock or working solutions depending on the medium (literature/general). Always confirm final pH and sterility of prepared media.

Quality and Grades

• Grade/Purity (from Product Data): BioReagent.

What BioReagent typically implies (general):

• Intended use: Suitable for laboratory research applications, commonly including microbiology, cell biology adjuncts, and bioprocess development. It is not certified for clinical or diagnostic use.
• Performance orientation: Emphasis on functional performance in media (growth promotion), low interfering contaminants for common lab assays, and batch-to-batch consistency within defined acceptance criteria. Specific numeric limits (e.g., ash, chloride, total nitrogen, microbiological bioburden, endotoxin) are often controlled but are Not specified for this item; refer to CoA/Spec Sheet.
• Animal-origin considerations: As a meat-derived digest, users may require TSE/BSE statements and origin traceability for regulated environments. Documentation availability is supplier- and lot-dependent; Not specified for this item.
• Filtration/sterility: Supplied non-sterile as a dry powder unless otherwise stated; Not specified for this item. Users should sterilize prepared media by autoclaving or 0.22 µm filtration as appropriate to heat sensitivity of other medium components.

Lot qualification (how to evaluate, general):

• Perform a growth promotion test (GPT) with representative organisms (e.g., E. coli, S. aureus, B. subtilis) in the target medium formulation.
• Compare optical density/CFU yield and lag time against in-house reference peptone or prior lot.
• Assess clarity, color, and pH of a standard solution (e.g., 10 g/L) pre- and post-sterilization.

Note: For any regulatory or QA needs, consult the CoA/Spec Sheet for item-specific specifications.

Reaction and Applications

Chemical reaction applications: This product is not typically used as a reagent or solvent in synthetic organic chemistry. It does not participate as a defined reactant in named reactions.

Biological/bioprocess applications (literature/general):

• Microbial culture media: Serves as a nitrogen and growth factor source in Nutrient Broth/Agar, enriched broths, and custom fermentation media. Supports growth of a broad range of bacteria by providing peptides, amino acids, vitamins, and minerals.
• Fermentation: Commonly included at 5–20 g/L to boost biomass and recombinant protein expression in microbial hosts; often used with yeast extract and defined salts.
• Diagnostic media base: Contributes to non-selective basal media used for colony enumeration, environmental monitoring, and sample enrichment prior to selective plating.
• Enzyme production: The peptide profile from pepsin digestion can influence secretion levels of certain microbial enzymes; empirical optimization is recommended.
• Buffering contribution: Provides minimal intrinsic buffering; final pH is set by added buffer/salts and organism metabolism.

Practical tips:

• Rehydrate in warm (37–50 C) water with gentle stirring to reduce foam.
• Filter through 0.45→0.22 µm if solution clarity is required; pre-wet membranes to minimize binding.
• Validate lot-to-lot performance via growth promotion tests using representative organisms and your target formulation.

All usage is for laboratory research purposes only.

Reaction Conditions

Classical reaction-condition guidance does not apply to this complex nutrient. Instead, the relevant conditions concern dissolution, sterilization, and use in culture media (literature/general):

• Concentration ranges: Commonly 5–20 g/L in basal media; optimize 2–40 g/L depending on organism and co-supplements.
• Dissolution: Add slowly to deionized water at room temperature to 50 C with gentle stirring. Avoid vigorous aeration to limit foaming.
• pH adjustment: Adjust pH after full dissolution and addition of other components. Typical bacterial media pH targets are ~6.8–7.4 (organism-dependent).
• Sterilization: Autoclave complete medium at 121 C for 15–20 min if compatible. For heat-sensitive formulations, 0.22 µm filter-sterilize the digest solution and combine aseptically with other sterile components.
• Compatibility: Mixes well with yeast extract, beef/meat extracts, tryptone/casein digests, and carbohydrates (glucose, glycerol, lactose). Avoid strong oxidants in solution.
• Storage of solutions: Use fresh when possible. For short-term storage, keep sterile solutions at 2–8 C for up to 1–2 weeks; monitor for turbidity or pH drift.
• Quality checks: Perform growth promotion tests against reference strains; monitor OD600 growth curves and CFU counts to verify performance with each lot.

All numerical ranges are general literature guidance and not item-specific specifications.

Safety and Handling

Item-specific hazard information:

• GHS Classification / Signal Word / H-Statements / Pictograms: Not specified for this item; refer to SDS.

General laboratory safety guidance (literature/general):

• PPE: Lab coat, safety glasses, and appropriate gloves. Avoid creating dust/aerosols; use local exhaust or handle in a biosafety cabinet if preparing sterile media.
• Inhalation of dust: May cause respiratory irritation or allergen-like responses in sensitive individuals. If inhaled, move to fresh air and seek medical advice if symptoms persist.
• Skin/eye contact: Rinse skin with water; flush eyes with water for several minutes. Remove contaminated clothing. Seek medical attention if irritation persists.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical advice.
• Spill response: Avoid dust generation; sweep or vacuum with HEPA filtration. Wash spill area with water.
• Fire behavior: Organic material; may burn if involved in a fire. Use water spray, CO2, foam, or dry chemical as appropriate for surrounding materials (consult SDS for specifics).
• Incompatibilities: Strong oxidizers; store away from moisture to avoid caking and microbial growth in opened containers.
• Microbiological considerations: Although supplied dry, the material is nutrient-rich; any wetted or reconstituted material should be treated as a potential microbial growth substrate. Prepare and handle solutions aseptically; sterilize by autoclaving or filtration as appropriate.

Always consult the product’s SDS for authoritative hazard, exposure controls, and first-aid measures.

Solvent Selection

As a protein hydrolysate intended for media preparation, the relevant solvent is water or aqueous buffers rather than organic solvents.

General guidelines (literature/general):

• Primary solvent: Deionized/distilled water. Typical working concentrations in media are 5–20 g/L depending on the formulation and organism.
• Buffer compatibility: Compatible with common biological buffers (e.g., phosphate, Tris) after dissolution; adjust pH after all components are in solution.
• Ionic strength: Sodium chloride (e.g., 5 g/L in Nutrient Broth) can be added to control osmolarity where required.
• Sterilization: Autoclave complete media if other components are heat-stable. Alternatively, dissolve at 37–50 C, filter sterilize (0.22 µm), and store at 2–8 C for short-term use.
• Avoid: Organic solvents; the product is largely insoluble in non-aqueous media and can precipitate in high-organic content solutions. Avoid strong acids/bases post-dissolution that may degrade peptides.

Comparison (when to choose alternatives):

• Meat Peptic Digest vs. Casein digests (tryptone/peptone): Meat digest may enrich hydrophobic/aromatic peptides and micronutrients native to muscle; casein digests provide a highly consistent amino-nitrogen profile.
• Animal-free peptones (soy/yeast/plant): Prefer for regulatory or animal-component–free processes; nutrient spectrum differs and may require re-optimization of medium composition.

Storage and Reconstitution

• Storage Conditions (from Product Data): Room temperature.
• Shipped In (from Product Data): Normal.
• Research Use Note: For research use only.

Reconstitution (general guidance):

• Use deionized or distilled water. Target working concentrations typically 5–20 g/L depending on the medium (literature/general).
• Add powder gradually with stirring to minimize clumping and foam. Mild warming (up to ~50 C) can expedite dissolution.
• Adjust pH after all components are dissolved.
• Sterilize by autoclaving with the complete medium when compatible, or sterile-filter the Meat Peptic Digest solution (0.22 µm) and combine aseptically.

Solution handling and stability (literature/general):

• Use freshly prepared solutions whenever possible.
• Store sterile solutions at 2–8 C, protected from light, and use within 1–2 weeks. Discard if turbidity, precipitation, or off-odor develops.
• Avoid repeated warming/cooling cycles; prepare aliquots of sterile stock if frequent use is anticipated.

Packaging and moisture control:

• Reseal containers promptly after opening. Protect from humidity to prevent caking and bioburden uptake.

Note: No item-specific specifications (e.g., ash, nitrogen content, microbial limits) are provided here; consult the product’s CoA/Spec Sheet for lot-specific parameters.

Structure and Identity

Overview: Meat Peptic Digest is an enzymatic hydrolysate of animal (meat) proteins produced using pepsin. It is an undefined, heterogeneous mixture of short peptides, free amino acids, and small peptides with varied side chains, along with trace minerals and other low–molecular-weight components derived from the source tissue.

• Composition type: Complex mixture (peptone/peptic digest of animal tissue)
• Defining features: Generated by acid protease (pepsin) cleavage, giving a spectrum enriched in hydrophobic and aromatic peptides relative to neutral protease digests; generally low in intact high-MW proteins due to extensive proteolysis.
• Functional groups present (mixture): Primary/secondary amides (peptide bonds), free amines (N-termini/lysine side chains), carboxylates (C-termini/aspartate/glutamate), imidazole, indole, phenolic, thiol, and other amino-acid side chains (literature/general).
• Stereochemistry: Derived from natural L-amino acids; peptides retain native stereocenters (literature/general).

Item-specific identifiers:

• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not applicable (mixture).
• Molecular Weight: Not applicable as a single value (broad distribution); average MW not specified for this item.
• SMILES: Not applicable (mixture).
• InChIKey: Not applicable (mixture).
• Category: Life Science reagent; nutrient/peptone for culture media (general).

2D structure description: Not applicable to a defined single compound; constituents are short peptide chains (typically 2–20 residues, literature/general) and free amino acids arising from pepsin-mediated cleavage of muscle proteins.

Synthetic Utility

In the context of laboratory use, Meat Peptic Digest does not serve as a defined synthetic building block or reagent. Its utility is operational and biological:

• Media formulation tool: Provides a broad spectrum of peptides and amino acids, enabling rapid prototyping of growth media for diverse microorganisms (literature/general).
• Nutritional balancing: Used to titrate total organic nitrogen (TON) in combination with yeast extract, casein digests, and defined salts to achieve target growth rates and productivities.
• Expression screening: Supports early-stage screens for recombinant protein production conditions in E. coli and other microbes prior to transitioning to defined or animal-free media.
• Assay compatibility: Complex matrices may interfere with colorimetric or fluorometric assays; pre-clarify and validate blank corrections as needed.

Chemistry note: Because it is a heterogeneous peptide mixture, it is unsuitable for stoichiometric transformations or reactions requiring defined molecular identity. For peptide chemistry or bioconjugation studies, use defined amino acids or purified peptides instead.

Bottom line: Treat this product as a functional nutrient supplement for microbiology/bioprocess workflows rather than as a chemical reagent in synthetic reactions.

Target Specificity

Not applicable. This product is not an antibody, probe, or ligand and has no defined molecular target specificity. It is a complex nutrient mixture intended to support growth of diverse microorganisms in culture media.