Modified Brucella Broth Medium - BioReagent

Cat. No.: M1528618

QUALITÉ & PURETÉ BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

For enrichment cultivation of Campylobacter jejuni

Spécifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Product Line

Produits biologiques

Application Protocols

No item-specific, validated application protocols are provided in the Product Data.

• Recommended preparation parameters, sterilization conditions, supplements, and incubation guidance: Not specified for this item; refer to the product label and CoA/Spec Sheet.

For laboratory use, follow institutional SOPs and organism-specific literature when employing Modified Brucella Broth Medium.

Biological Roles

Modified Brucella Broth Medium is designed for cultivating fastidious and non-fastidious bacteria in research laboratories (no clinical/diagnostic use claimed).

• General functional features (literature/general):

  • Nutritional base: Peptones/tryptone supply peptides, amino acids, and growth factors; yeast extract contributes vitamins, nucleotides, and trace elements.
  • Energy sources: Carbohydrates (e.g., dextrose) may be included to support fermentative metabolism and biomass accumulation.
  • Buffering: Phosphate or other buffers help maintain near-neutral pH during growth.
  • Modified Brucella formulations are historically used for cultivating organisms requiring enriched conditions and for anaerobic culture workflows when combined with appropriate supplements and reducing conditions.

• Research contexts (general):

  • Enrichment prior to downstream molecular analyses, biochemical assays, or phenotype characterization.
  • Preparation of inocula for fermentation or susceptibility research under controlled, non-clinical settings.

Exact composition, presence/absence of supplements, and targeted organisms for growth-promotion testing are not specified for this item; refer to the CoA/Spec Sheet for definitive details.

Buffer Applications

This product is a complex nutrient medium rather than a defined buffering system.

• Item-specific buffer/pH details: Not specified for this item; refer to CoA/Spec Sheet.

• General notes (literature/general):

  • Brucella-type broths typically include buffering salts to maintain culture pH during microbial growth, often targeting a neutral pH range.
  • For experiments that require tight pH control independent of metabolic acid/base production, pair the medium with additional buffering systems (e.g., HEPES) as appropriate to the organism and study design.

Green Alternatives

Green chemistry considerations for microbiological media focus on packaging, water/energy use, and waste handling rather than solvent substitution.

• General sustainability options (literature/general):
  • Concentrated/dehydrated format vs. ready-to-use: Dehydrated powders reduce shipping weight and packaging volume, lowering footprint; prepare only the volume needed to minimize waste.
  • Sterilization efficiency: Use validated autoclave cycles and load planning to reduce energy consumption while ensuring sterility.
  • Waste minimization: Segregate uncontaminated packaging from biohazardous waste; neutralize/disinfect spent media before disposal following local regulations.

Comparison (general):

• Dehydrated medium (this product category)
  • Pros: Lower shipping mass, longer shelf-life at room temperature, flexible batch sizing.
  • Tradeoffs: Requires in-lab water, energy for sterilization, and aseptic handling.
• Ready-to-use liquid media
  • Pros: Time-saving, consistent; no reconstitution variability.
  • Tradeoffs: Higher shipping weight/volume, refrigerated transport/storage often required, shorter shelf-life once opened.

Pharmaceutical Uses

No excipient or formulation roles are specified for this product.

• Intended use: For research use only (from Product Data). Not for clinical, diagnostic, or therapeutic applications.

• General industry context (literature/general):

  • Complex media such as Modified Brucella Broth can be used in non-clinical pharmaceutical microbiology research, method development, and environmental isolate studies where enriched growth is desirable.
  • Any compendial status, if applicable, must be verified on the CoA/Spec Sheet; none is specified here.

Physical Properties

Item-specific physical specifications have not been provided for this catalog entry.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet. (Typical dehydrated media are free-flowing powders; literature/general.)
• Solubility: Not specified for this item; refer to CoA/Spec Sheet. (Dehydrated media are generally water-soluble; literature/general.)
• pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet. (Brucella-type broths often target pH near neutrality at 25 °C; literature/general.)
• BP/MP, density, refractive index, logP, pKa: Not applicable to a complex dehydrated blend.

Always consult the specific CoA/Specification Sheet for this lot for any target pH, preparation mass per liter, and clarity requirements.

Quality and Grades

• Item-specific facts:

  • Grade/Purity: BioReagent (from Product Data)

• What BioReagent grade typically implies (general):

  • Suitable for biological and microbiological research workflows where consistent nutrient performance and low inhibitory contaminants are important.
  • Vendor-controlled raw material sourcing and blending to meet internal performance criteria (e.g., recovery/growth characteristics, clarity), though exact specs must be confirmed on the CoA/Spec Sheet for this item.

• Stabilizers/additives:

  • Not specified for this item; refer to CoA/Spec Sheet.

• Performance verification (general guidance):

  • For complex media, vendors often verify growth promotion using defined strains and incubation conditions. Specific test organisms and acceptance ranges are not provided here; consult the CoA for this lot.

Reaction and Applications

This product is a culture medium and is not used as a reagent for synthetic organic or inorganic reactions.

• Not applicable: Named chemical reactions, catalysis, or stoichiometric transformations.
• Relevant application domain instead:
  • Microbiological culture and enrichment in research settings. See Biological Roles for context and Application Protocols (if provided) for preparation/usage specifics.

For chemical synthesis needs, refer to appropriate reagents and solvents rather than microbiological media.

Reaction Conditions

Not applicable to chemical reactions.

• Microbiological cultivation conditions (item-specific values not provided): Not specified for this item; refer to CoA/Spec Sheet and laboratory SOPs.

• General guidance (literature/general):

  • Reconstitution with purified water to a specified mass-per-liter, followed by sterilization (e.g., autoclaving) and, when required, supplement addition under aseptic conditions.
  • Incubation parameters (temperature, atmosphere, agitation) depend on the organism under study; consult organism-specific literature.

Always follow biosafety and institutional guidelines for culture handling and disposal.

Safety and Handling

No GHS hazard classification is provided in the Product Data for this medium. Treat as laboratory chemical and defer to the SDS for authoritative guidance.

• GHS/Signal Word/Pictograms/H-Statements: Not specified for this item; refer to SDS.
• General laboratory precautions (general guidance):
  • Use appropriate PPE: lab coat, gloves, and eye protection when handling dehydrated powder and prepared broth.
  • Avoid inhalation of dust; handle powders in a fume hood or with local exhaust if dusting may occur.
  • Prevent microbial contamination and inadvertent culture of unknown organisms; practice aseptic technique when preparing and using the medium.
  • Autoclave or appropriately disinfect spent media and materials before disposal per institutional biosafety procedures.
• Incompatibilities and special risks (general):
  • Keep dry; exposure to moisture can cause clumping and degradation of performance.
  • Store away from strong oxidizers and sources of contamination. Prepared media may support microbial growth—handle as a biohazard once inoculated.
• First aid (overview; defer to SDS):
  • Inhalation of dust: move to fresh air; seek medical advice if irritation persists.
  • Skin/eye contact: rinse with water for several minutes; remove contaminated clothing; seek medical attention if irritation continues.
  • Ingestion: rinse mouth; seek medical advice.

For detailed hazard, spill, and firefighting measures, consult the product-specific SDS.

Solvent Selection

As a dehydrated microbiological medium, this product is intended to be reconstituted in purified water; selection among organic solvents is not applicable.

• Item-specific preparation solvent: Not specified for this item; refer to CoA/Spec Sheet.

• General guidance (literature/general):

  • Use high-purity water (e.g., distilled, deionized) appropriate for microbiological media preparation.
  • Avoid solvents and additives that could inhibit microbial growth (e.g., residual alcohols, strong detergents).
  • pH adjustment, if required by the specification, should be performed with sterile 1 N acid/base, followed by sterilization (typically autoclaving), as directed on the product’s instructions.

If a particular mass-per-liter or pH is required, please consult the product label or CoA for reconstitution parameters.

Storage and Reconstitution

• Storage (item-specific): Room temperature (from Product Data). Protect from moisture and contamination; keep container tightly closed. Shipped under normal conditions (from Product Data).
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet for mass-per-liter, pH targets, sterilization, and any supplement/additive instructions.
• Stability (general guidance):
  • Dehydrated media are generally stable at ambient temperature when kept dry and sealed; avoid high humidity and temperature extremes.
  • Prepared media typically have limited shelf-life and may require refrigeration; follow CoA/label directions when available.

Structure and Identity

Modified Brucella Broth Medium is a complex, dehydrated microbiological culture medium, not a single defined small molecule.

• Item-specific identifiers from Product Data:

  • SKU: M1528618
  • Product Name: Modified Brucella Broth Medium
  • Grade/Purity: BioReagent
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Formula: Not applicable (complex mixture of organic/inorganic components).
  • Molecular Weight: Not applicable (mixture).
  • SMILES / InChI / InChIKey: Not applicable for complex media.

• Structural description (general knowledge):

  • Composed of multiple constituents typically including peptones/tryptones (polydisperse peptide/AA fragments), yeast extract (vitamins, nucleotides), dextrose or other carbohydrates, mineral salts, and buffering components. Some modified formulations may include supplements or altered nutrient ratios (literature/general).
  • Being a complex mixture, it lacks a defined 2D/3D molecular structure; properties are emergent from the blend of components (general).

Synthetic Utility

Not applicable. Modified Brucella Broth Medium is not a reagent for chemical synthesis or a building block.

• For synthetic chemistry applications, select defined reagents, catalysts, and solvents appropriate to your transformation. This medium is intended for cultivating microorganisms in research settings.

Target Specificity

No antibody, enzyme, or molecular target specificity is applicable to this product type.

• Tested organisms or growth-promotion specifics: Not specified for this item; refer to CoA/Spec Sheet.