MT-DADMe-ImmA - 10mM in DMSO , CAS No.653592-04-2

CAS: 653592-04-2 Cat. No.: M654772 Formule: C₁₃H₁₉N₅OS Poids moléculaire: 293.39 PubChem CID: 656970

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

MT-DADMe-ImmA is an inhibitor of human 5'-methylthioadenosine phosphorylase ( MTAP ) with a K i of 90 pM.

In Vitro

Treatment of cultured cells with MT-DADMe-ImmA and MTA inhibit MTAP, increase cellular MTA concentrations, decrease polyamines, and induce apoptosis in FaDu and Cal27, two head and neck squamous cell carcinoma cell lines. The same treatment does not induce apoptosis in normal human fibroblast cell lines (CRL2522 and GM02037) or in MCF7, a breast cancer cell line with an MTAP gene deletion. MT-DADMe-ImmA alone does not induce apoptosis in any cell line, implicating MTA as the active agent. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The t 1/2 for onset of inhibition is 50 min with complete inhibition by 250 min. MTAP activity slowly returns, giving a biological half-life for the action of oral MT-DADMe-ImmA of 6.3 days. The time-dependent growth of FaDu tumors in immunodeficient mice is suppressed by oral or intraperitoneal treatment with MT-DADMe-ImmA. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay

Cell viability is evaluated using the Alamar Blue assay. Cells are seeded onto 96-well plates at a density of 104 cells/well and incubated with increasing concentrations of MT-DADMe-ImmA (100 pM to 100 μM) for 4 days at fixed MTA concentrations (0, 5, 10, and 20 μM). IC 50 is determined with the assay data. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:Ki: 90 pM (MTAP)

Specifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

MT-DADMe-ImmA is an inhibitor of human 5'-methylthioadenosine phosphorylase ( MTAP ) with a K i of 90 pM.

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Type d'action

INHIBITOR

Noms et identifiants

• Isomères SMILES: CSC[C@H]1CN(C[C@@H]1O)CC2=CNC3=C2N=CN=C3N
• PubChem CID: 656970
• Poids moléculaire: 293.39

Cibles associées (humaines)

MTAP Tchem S-methyl-5'-thioadenosine phosphorylase (4 Activities)

Discover Target: MTAP

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 653592-04-2, the molecular formula is C13H19N5OS, and the molecular weight is 293.39 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.