Mz325 , CAS No.3032670-12-2

CAS: 3032670-12-2 Cat. No.: M1426661 Formule: C₃₈H₄₅N₉O₅S₂ Poids moléculaire: 771.95

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Vue d’ensemble

Mz325 is a dual inhibitor of HDAC and Sirt2 , with the IC 50 of 9.7 μM to Sirt2 , that play an important role in pathogenesis of cancer and neurodegeneration.

Specifications

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Type d'action

INHIBITOR

Noms et identifiants

Poids moléculaire

771.95

Application Protocols

Item-specific

• Tested applications, recommended working concentrations/dilutions, and positive controls: Not specified for this item.

General note

• Protocols vary widely by assay type. In the absence of item-specific guidance, develop assay conditions using internal controls and pilot titrations. For stock handling suggestions, see Storage & Reconstitution.

Biological Roles

Item-specific

• Biological target, pathway involvement, and mode of action: Not specified for this item; refer to any available technical note or CoA.

General context (for small-molecule library members)

• Without structural and mechanistic disclosure, no biological function can be ascribed. Such compounds are commonly evaluated empirically across biochemical or cell-based panels to identify activity, followed by deconvolution to exclude artifacts (fluorescence quenching, redox cycling, aggregation).
• Recommended best practices (general):
  • Confirm purity/identity prior to biological testing.
  • Assess compound stability in the intended assay matrix (e.g., buffer ± cofactors, serum-containing media) over the assay timeframe.
  • Monitor for aggregation by detergent sensitivity assays or DLS where feasible.
  • Use orthogonal assays to validate initial hits and rule out assay interference.

Note: For research use only. No clinical or diagnostic use is implied.

Buffer Applications

Item-specific

• Buffer usage, buffering capacity, and pKa: Not specified for this item; the compound is not presented as a buffer reagent.

General guidance (if using in aqueous assays)

• Prepare concentrated stocks in a water-miscible organic solvent (commonly DMSO), then dilute into assay buffers while keeping the final organic content low (often 0.1–1.0% v/v; verify compatibility with your assay system).
• To mitigate precipitation on dilution, add compound stock last while vortexing, and consider inclusion of a small amount of non-ionic surfactant (e.g., 0.01–0.05% Tween-20) if compatible with the assay; confirm that the surfactant does not affect readouts.
• Filter-sterilization of final working solutions (0.22 µm) may be used for cell-based work if solubility allows and the compound is not adsorbed by the membrane.

Green Alternatives

Item-specific

• No primary application or solvent system is specified for Mz325; green alternatives cannot be assigned to the item specifically.

General considerations (literature/principles)

• If preparing stocks in DMSO, consider minimizing DMSO volumes in the final assay (≤0.1–0.5% v/v where feasible) and evaluate bio-based or safer co-solvents when compatible with the assay (e.g., PEG-400 or glycerol-water co-solvent systems), noting potential impacts on permeability and binding.
• For synthetic handling (if applicable), greener solvent options may include 2-MeTHF over THF, CPME over diethyl ether, EtOAc over DCM, and MeOH/EtOH over isopropanol/ACN where performance allows.

Illustrative comparison (general)

• DMSO vs PEG-400: DMSO offers superior solvency and analytical cleanliness; PEG-400 has lower volatility and improved safety profile but higher viscosity and UV background.
• ACN vs EtOH: ACN provides sharper LC peaks and low viscosity; EtOH is greener but can broaden peaks and reduce MS sensitivity.

Pharmaceutical Uses

Item-specific

• Regulatory/compendial status, GMP suitability, and excipient use: Not specified for this item.

General statement

• This product is provided strictly for research use only (as stated). It is not intended for human or veterinary use, clinical diagnostics, or as a drug substance/excipient in commercial manufacturing. No pharmacopeial compliance is claimed.
• For pre-formulation research, if applicable, typical data to gather include salt/counter-ion screening, polymorph evaluation (XRPD), and stability under ICH conditions—none of which are specified for this item.

Physical Properties

Item-specific (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility profile: Not specified for this item; refer to CoA/Spec Sheet.
• logP/logD, pKa: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice)

• In absence of a disclosed structure, determine solubility empirically across a small solvent panel (e.g., DMSO, DMF, MeOH, EtOH, acetonitrile, buffers at pH 6–8) and monitor by visual inspection and HPLC.
• For thermal behavior, DSC/TGA can provide onset of melting/decomposition; these are useful for formulation and storage planning.
• If UV-active, a quick UV–Vis scan (190–400 nm) helps select HPLC wavelengths; exact UV cutoff is unknown for this item.

Quality and Grades

Item-specific (from Product Data)

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Context and expectations (general information for small-molecule libraries)

• Screening compounds are commonly provided at synthetic or research grade with HPLC purity typically reported on the CoA (e.g., >90–98% by area), along with identity confirmation by LC–MS and/or NMR. Because no grade is listed here, do not assume a specification—consult the item’s CoA.
• UV absorbance, residual solvent content, water content (Karl Fischer), and salt form are critical for assay reproducibility but are not specified for this item; verify on receipt if your workflow is sensitive to these parameters.
• If a stabilizer, counter-ion, or salt form is used, it will appear on the label/CoA. None are specified here.
• For quantitative work, verify purity and identity in-house (single-point HPLC check, HRMS, or 1H NMR) before use in SAR or potency comparisons.

Reaction and Applications

Item-specific

• Manufacturer Applications: Not specified in the provided data.

Relevance

• Mz325 is listed under a small-molecule/compound library category. Such items are typically intended as screening hits/leads, tool compounds, or diversity set members rather than as stoichiometric reagents or solvents.

General application contexts (non-item-specific)

• Chemical biology and screening: evaluation in enzymatic, receptor-binding, or phenotypic assays to identify activity; follow-up SAR requires verifying identity/purity first.
• Probe development workflows: preliminary assessment of permeability, stability in buffer/microsomes (if applicable), and orthogonal assay confirmation to rule out assay interference. Without a disclosed structure, pan-assay interference (PAINS) filtering cannot be performed; use orthogonal controls.
• Analytical applications: establish LC–MS methods (e.g., gradient in water/ACN with 0.1% formic acid) for quantitation and stability-indicating analyses.

Note: No synthetic or named reaction roles are claimed for this specific item due to absent structural information.

Reaction Conditions

Item-specific

• Typical solvents, catalysts, temperatures, and yields: Not specified for this item; no reaction use is indicated.

General note

• Because Mz325 is offered as a small-molecule library member and its structure is undisclosed here, standardized reaction conditions are not applicable. If you intend to use it as a substrate, independently establish conditions guided by its functional groups after obtaining structural information (consult CoA or request a technical dossier if available).

Safety and Handling

Item-specific (from Product Data)

• GHS Classification: Not specified for this item; refer to SDS.
• Signal Word / H-Statements / Pictograms: Not specified for this item; refer to SDS.
• Storage Conditions: Store at −20°C.
• Shipped In: Ice chest + ice pads.

General precautions (always defer to the SDS for authoritative guidance)

• Handle as a potentially hazardous laboratory chemical. Avoid inhalation, ingestion, and skin/eye contact. Use in a chemical fume hood.
• Recommended PPE: lab coat, safety glasses or goggles, and appropriate chemically resistant gloves (e.g., nitrile). For solids/powders, minimize dust; for solutions, avoid aerosol formation.
• Incompatibilities: Unknown for this specific item; as a prudent practice, keep away from strong oxidizers/acids/bases until compatibility is known.
• First aid overview: In case of skin contact, wash with soap/water; eye exposure, rinse with water for at least 15 minutes and seek medical attention; inhalation, move to fresh air; ingestion, seek medical attention. Provide the SDS to responders.
• Waste: Collect as organic chemical waste; segregate halogenated vs non-halogenated streams as appropriate.
• Special risks: Structure not specified; if supplied as a dry solid, consider potential dust explosivity and hygroscopicity as unknown—store tightly closed, desiccated, and protected from light until more data are available.

Solvent Selection

Item-specific

• Solubility/miscibility: Not specified for this item; refer to CoA/Spec Sheet.

General selection guidance for small-molecule stock preparation (literature/practice)

• Primary solvent for screening stocks: DMSO is broadly effective for diverse chemotypes and is miscible with water and common assay buffers in small volumes.
• Alternative solvents to test: DMF, methanol, ethanol, acetonitrile. For hydrophobic chemotypes, consider co-solvent systems (e.g., DMSO + PEG-400 or DMSO + Tween-80 at low v/v%) after confirming assay compatibility.
• Practical workflow:
  • Begin with dry, anhydrous DMSO; target 10–50 mM stock solutions if solubility permits.
  • Perform a small solubility screen: weigh ~1 mg, add 20–100 µL solvent, sonicate/warm gently (<40°C) as needed; inspect for clarity and re-crystallization upon cooling/dilution.
  • Check for precipitation upon dilution into assay media (typical final DMSO 0.1–1.0% v/v in biochemical assays; confirm tolerance for your system).

Quick comparison (general)

• DMSO: highest solvency for organics; cell-compatible at low %; hygroscopic and can carry water.
• DMF: strong solvent; higher toxicity; less preferred in bioassays.
• MeOH/EtOH: volatile, assay-compatible in small %; limited solvency for very lipophilic compounds.
• ACN: good for analytical prep; moderate solvency; volatile.

Storage and Reconstitution

Item-specific (from Product Data)

• Storage Conditions: Store at −20°C.
• Shipping: Ice chest with ice pads to maintain low temperature.

General best practices (literature/practice; verify against your CoA)

• Container: Keep tightly sealed in an inert, dry container. Protect from light and moisture until use.
• Reconstitution: If solid, prepare a concentrated stock in dry, high-purity DMSO (e.g., 10–50 mM as solubility permits). If supplied as a solution, record the exact concentration and solvent on receipt.
• Aliquoting: Divide into single-use aliquots (e.g., 10–50 µL) to minimize freeze–thaw cycles. Store aliquots at −20°C (or per CoA). Avoid frost-free freezers that cycle temperature.
• Thawing/handling: Allow to warm briefly to room temperature before opening to prevent moisture condensation. Vortex to homogenize; inspect for crystals/precipitate. If precipitation occurs, warm gently and sonicate; do not exceed ~40°C unless stability is known.
• Stability/shelf life: Not specified for this item; refer to CoA/Spec Sheet. For in-use stability, prepare fresh working dilutions daily unless stability has been established.
• Documentation: Record lot number, preparation date, solvent, and concentration on aliquot labels for traceability.

Structure and Identity

Item-specific (from Product Data)

• SKU: M1426661
• Product Name: Mz325
• CAS: 3032670-12-2
• Category: Small molecules and compound libraries (catalog category: 全部 / 可售 / 小分子和化合物库)
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

General notes (chemistry context)

• Structure: Not disclosed in the provided data. Without a structure, functional groups, ring systems, stereochemistry, and 2D depiction cannot be described.
• Identity confirmation (typical practice, literature/general): Identity for small-molecule screening members is commonly supported by LC–MS (exact mass), 1H/13C NMR, and HPLC purity; please consult the product-specific CoA for this item.

Synthetic Utility

Item-specific

• Structural features and reactive handles enabling transformations: Not specified for this item; no structure provided.

General note

• As listed, Mz325 is positioned as a screening compound/library member rather than a building block or reagent. Without structural information, no synthetic transformations, protecting-group strategies, or named-reaction roles can be responsibly recommended.

Target Specificity

Item-specific

• Target/antigen, binding site/epitope, species selectivity, and related bioactivity data: Not specified for this item.

Note

• No target specificity claims can be made without provided biological data. If you require target annotations, please contact us with the SKU to request any available technical notes.