Necrostatin 2 - 10mM in DMSO , CAS No.852391-19-6

CAS: 852391-19-6 Cat. No.: N654511 Formule: C₁₃H₁₂ClN₃O₂ Poids moléculaire: 277.7 Numéro CE: 813-204-7

QUALITÉ & PURETÉ 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

Necrostatin 2 is a potent necroptosis inhibitor. EC 50 for inhibition of necroptosis in FADD-deficient Jurkat T cells treated with TNF-α is 0.05 μM.Necrostatin 2 is also a RIPK1 inhibitor.

In Vitro

Evaluation of necroptosis inhibitory activity is performed using a FADD-deficient variant of human Jurkat T cells treated with TNF-α. Utilizing these conditions the cells efficiently undergo necroptosis, which is completely and selectively inhibited by Necrostatin 2 (EC 50 =50 nM). Necrostatin 2 shows activity in a broad range of necroptosis cellular systems. Necrostatin 2 at 30 μM completely protects L929 cells from TNF-α-induced necroptosis. In addition to TNF-α, the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone (zVAD.fmk) has also been found to induce necrosis in L929 cells, which is efficiently inhibited by Necrostatin 2. EC 50 for inhibition of necroptosis in FADD-deficient Jurkat T cells treated with TNF-α is 0.05 μM. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay

L929 cells (100000 cells/mL, 100 μL/well in a 96-well plate) are treated with 10 ng/mL human TNF-α or 100 μM zVAD.fmk in the presence of DMSO (control), 30 μM Necrostatin 2, or 8for 24 h at 37°C in a humidified incubator with 5% CO 2 followed by ATP-based viability assessment as described in the previous experiment. Stock solutions (30 mM) in DMSO are initially prepared and then diluted with DMSO to give testing solutions. Each sample is done in duplicate. The final DMSO concentration is 0.5%. Cell viability values are adjusted to account for nonspecific toxicity, which in most cases is <10%. The reported cell viability values (%)±SD are determined from two independent experiments. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:Necroptosis, RIPK1

Spécifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

Necrostatin 2 is a potent necroptosis inhibitor. EC 50 for inhibition of necroptosis in FADD-deficient Jurkat T cells treated with TNF-α is 0.05 μM.\n\n\n\n\n Necrostatin 2 is also a RIPK1 inhibitor.

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Type d'action

INHIBITOR

Noms et identifiants

Isomères SMILES

CN1C(=O)[C@H](NC1=O)CC2=CNC3=C2C=CC=C3Cl

Poids moléculaire

277.7

Reaxy-Rn

10175735

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=10175735&ln=

Cibles associées (humaines)

RIPK1 Tchem Receptor-interacting serine/threonine-protein kinase 1 (1 Activities)

Discover Target: RIPK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

No tested/validated application protocols are provided for this SKU.

• Recommended applications, dilutions, or positive controls: Not specified for this item; refer to CoA/Spec Sheet and peer-reviewed literature relevant to your assay system.
• General recommendation: Perform a small pilot experiment to define working concentrations, solvent tolerance, exposure time, and readouts; include matched vehicle controls and orthogonal pathway modulators as appropriate.

For standardized protocols, consult domain-specific literature and adapt to your instrumentation and cell/biochemical system.

Biological Roles

Research context (literature, not item-specific claims):

• Necrostatin-class molecules are widely used tool compounds in the study of regulated necrosis (necroptosis) signaling. They are commonly employed to probe kinase-dependent steps upstream in necroptotic pathways, enabling dissection of biochemical events distinct from apoptosis.
• In cell-based models, such compounds are used to differentiate pathway involvement by assessing rescue of cell viability, changes in biomarker readouts, or modulation of downstream effectors under necrosis-inducing conditions.
• In biochemical assays, they serve as reference ligands for characterizing enzyme activity, selectivity, and kinetics in necrotic signaling cascades.

Use considerations:

• Biological activity can be context-dependent across cell type, species, and assay design. Establish appropriate positive/negative controls, including orthogonal pathway modulators, to confirm on-pathway effects.
• Validate concentration ranges to balance activity against off-target or solvent effects. Perform titration series and replicate testing.

Important: This product is for research use only. No medical, diagnostic, or therapeutic use is intended or permitted. Experimental outcomes are model- and protocol-dependent and should be interpreted alongside appropriate standards and controls.

Buffer Applications

This product is not a buffering agent. However, it is often used in buffered biological media as a dissolved small molecule.

General, non-item-specific guidance:

• Stock preparation: Dissolve in DMSO to a convenient concentration (e.g., 10–50 mM), then dilute into PBS, HBSS, or culture media with vigorous mixing to the desired final concentration.
• Final solvent content: Keep DMSO ≤0.1–0.5% v/v to limit solvent effects on cells or enzymes; include matched vehicle controls.
• Order of addition: Add the DMSO stock last to well-mixed buffer/media to reduce local supersaturation and precipitation.
• Filtration: If sterilization is required, use low-binding filters and verify compound recovery and stability post-filtration.

Note: Specific pKa or buffer-interaction data for this item are not provided. Confirm compatibility with your exact buffer composition (salts, proteins, detergents) empirically.

Green Alternatives

Because this product is a bioactive small molecule typically used as a reference tool in assays rather than as a bulk solvent or reagent, green chemistry considerations focus on solvent choice for stock solutions and assay dilutions.

Greener handling considerations (general guidance):

• Prefer DMSO over DMF for stock solutions when feasible—DMSO is lower hazard and has a favorable environmental profile relative to many dipolar aprotic solvents.
• Keep final organic solvent content minimal (≤0.1–0.5% v/v) in aqueous assays to reduce solvent waste and cytotoxicity.
• Use microscale preparation to limit excess solution disposal; adopt multiwell plate formats that minimize dead volume.

Comparison (general, not item-specific):

• DMSO: Low volatility, miscible with water, comparatively benign; widely accepted in bioassays.
• Ethanol: Renewable origin possible; compatible at low percentages; watch for biological effects at higher doses.
• DMF/Acetonitrile: Effective solvents but higher regulatory/safety burdens; reserve for cases where solubility demands it.

Operational practices:

• Implement sealed storage (crimped vials) and aliquoting to extend shelf-life and reduce waste.
• Use adsorption-minimizing plastics or glass to improve recovery and lower the need for re-preparation.

Always confirm compound stability in the chosen solvent system before adoption.

Pharmaceutical Uses

No pharmacopeial or excipient status is provided for this item; it is offered strictly for research use only.

Context (general, not item-specific):

• Compounds of this class are typically utilized as reference standards or tool molecules during discovery-stage research (e.g., target validation, pathway interrogation, assay controls) rather than as formulated drug substances.
• In pharmaceutical R&D workflows, such molecules may be included in screening decks, mechanism-of-action studies, and structure–activity relationship campaigns.

This listing includes no claims of suitability for human or veterinary use, no GMP status, and no clinical application. For cGMP material or regulated-use requirements, contact Aladdin Scientific to discuss feasibility and documentation needs.

Physical Properties

Item-specific physicochemical specifications are not provided in this listing. Consult the CoA/SDS for definitive values.

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not applicable/unknown for a non-volatile solid; item-specific value not specified.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
• LogP, pKa, refractive index: Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance (not item-specific):

• Small-molecule tool compounds of this class are typically prepared as concentrated DMSO stock solutions (e.g., 10–50 mM) due to favorable solubility in polar aprotic solvents. Actual solubility of this item should be verified experimentally.
• For aqueous use, stocks are commonly diluted into buffered media with appropriate co-solvent (DMSO or ethanol) while keeping final organic content low (≤0.1–0.5% v/v). Perform a small-scale solubility test to confirm clarity and absence of precipitation.

Important: Do not treat the above general guidance as product specifications. Always defer to the item’s CoA and SDS for authoritative physical data.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and guidance:

• In the absence of an explicitly stated grade, users should refer to the CoA for assay purity, identity confirmation (e.g., HPLC/UPLC, NMR, MS), and limits for residual solvents or related substances.
• For screening and mechanistic biology work, researchers commonly require ≥95% HPLC purity with clear single-peak chromatograms and identity confirmed by HRMS and 1H NMR. Verify these on the provided CoA for this lot.
• If UV transparency is important (e.g., kinetic reads at specific wavelengths), confirm UV-Vis profile and any stabilizers listed on the CoA.
• If enantiomeric composition is relevant, ensure chiral purity/er is provided (if applicable); otherwise assume racemic/achiral as per CoA.

Stabilizers and additives:

• Stabilizer/antioxidant content: Not specified for this item; refer to CoA/Spec Sheet.
• Water content and residual solvents: Not specified for this item; refer to CoA/Spec Sheet.

Recommendation:

• Document batch/lot number in all notebooks and publications. For sensitive assays, perform incoming QC (e.g., confirmatory HPLC-MS) to align with lab’s acceptance criteria.

Reaction and Applications

Primary use context: This compound is supplied as a research tool molecule in small-molecule/compound libraries rather than as a synthetic reagent.

Applications (research-oriented; literature-based, not item-specific):

• Chemical biology: Employed as a tool in studies of regulated cell death pathways to interrogate kinase-dependent necrotic signaling in cells and lysates.
• Screening/assay development: Used as a positive control or reference compound in biochemical kinase activity assays and cell viability assays where necrotic vs apoptotic outcomes are differentiated.
• Probe benchmarking: Provides a comparator alongside related chemotypes (e.g., other necrostatins) to assess structure–activity relationships and pathway dependence.

Practical tips:

• Prepare fresh DMSO stocks; avoid repeated freeze–thaw by aliquoting. Confirm potency window and solvent tolerance in your specific assay format.
• Include vehicle controls matching the highest DMSO percentage and run concentration–response curves with adequate replicates.
• Monitor precipitation/turbidity after dilution into aqueous buffers or media; adjust dilution protocol to maintain clarity.

Note: This product is not positioned for use in named organic transformations or as a coupling partner. For synthetic chemistry needs, refer to building blocks and reagents optimized for reactivity and scalability.

Reaction Conditions

Not applicable to this product’s primary use. It is not intended as a reagent for synthetic transformations.

If used indirectly in chemistry workflows (general guidance):

• Analytical handling: Prepare solutions in HPLC-grade DMSO, acetonitrile, or methanol for LC-MS or HPLC analysis; filter through 0.2 µm PTFE/RC if compatible.
• Stability checks: Assess solution stability at room temperature and 4 °C over several hours; avoid prolonged exposure to light/air if the scaffold is known to be sensitive (consult SDS/CoA).

For actual reaction condition development, select dedicated reagents and catalysts appropriate to the transformation and consult primary literature.

Safety and Handling

Use standard precautions for handling research chemicals. Specific GHS data were not provided for this listing—consult the SDS for authoritative hazard classification.

• GHS Classification/Pictograms: Not specified for this item; refer to SDS.
• Signal Word/H-Statements: Not specified for this item; refer to SDS.

Best-practice PPE and engineering controls:

• Wear lab coat, appropriate chemically resistant gloves (e.g., nitrile), and safety glasses or face shield.
• Handle powders in a fume hood or ventilated enclosure to avoid inhalation of dust/aerosol.
• Avoid skin/eye contact and ingestion; wash thoroughly after handling.

Storage and incompatibilities:

• Storage condition (provided): Store at −80 °C.
• Shipments: Dry ice packs + cold packs (provided).
• Keep tightly closed in the original, light-protective container where applicable; minimize freeze–thaw by aliquoting upon receipt.
• Avoid strong oxidizers and strong acids/bases unless stability is documented in the SDS/CoA.

First-aid overview (consult SDS for details):

• Inhalation: Move to fresh air; seek medical attention if symptoms occur.
• Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; obtain medical advice if irritation persists.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Waste disposal:

• Collect and dispose according to institutional hazardous waste protocols; do not discharge to drains. Defer to SDS for proper classification.

Solvent Selection

This product is a small organic molecule typically handled as concentrated stock solutions for biochemical or cell-based studies.

General, literature-based solvent guidance (not item-specific specifications):

• Primary solvent: DMSO is commonly used due to broad solvating power and compatibility with biological assays at low final percentages (≤0.1–0.5% v/v).
• Secondary options: DMF or ethanol may dissolve similar chemotypes; verify compatibility with your assay and ensure final organic content is minimized.
• Aqueous media: Prepare a clear DMSO stock, then dilute into buffer or culture media under vigorous mixing. Use surfactant-free protocols unless warranted by the assay.

Polarity/miscibility notes (general):

• DMSO: Highly polar aprotic; fully miscible with water; often preferred for screening libraries.
• Ethanol: Protic; miscible with water; may impact membrane integrity at higher percentages—validate in controls.

Selection tips:

• Start with DMSO stock (e.g., 10–50 mM), assess clarity after dilution to the highest test concentration.
• If precipitation occurs, reduce final concentration, increase mixing time, or consider a cosolvent sequence (DMSO stock → buffer containing ≤0.5% DMSO).
• Filter sterilize diluted working solutions only if compound stability and nonspecific binding to membranes are acceptable (test recovery).

Always verify item-specific solubility and compatibility empirically and against the CoA/SDS.

Storage and Reconstitution

Storage conditions (item-specific):

• Store at −80 °C (per product data).
• Shipped on dry ice packs + cold packs to maintain low temperature.

General handling and reconstitution guidance (not item-specific specifications):

• Upon receipt: Allow the sealed container to equilibrate to room temperature in a desiccator before opening to minimize moisture condensation.
• Aliquot immediately to single-use vials to avoid repeated freeze–thaw cycles; keep headspace minimal.
• Stock solutions: Prepare in an appropriate solvent (commonly DMSO for similar small molecules). Use anhydrous solvent where possible and record the exact concentration.
• Storage of solutions: Freeze aliquots at −80 °C, protected from light. Clearly label with concentration, solvent, and preparation date. Avoid more than 1–2 freeze–thaw cycles.
• Stability: Item-specific shelf-life and solution stability are not specified; verify by LC/MS or HPLC prior to critical experiments.

Always defer to the CoA and SDS for definitive storage limits, stability data, and reconstitution instructions.

Research Use Note: For research use only.

Structure and Identity

A small-molecule research compound supplied for use in chemical biology and screening studies.

• SKU: N654511
• Product Name: Necrostatin 2
• CAS: 852391-19-6 (literature identifier for the small-molecule commonly known as “Necrostatin-2”)
• CID: 11288934 (literature)
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.

Structural description (general, literature):

• Necrostatins are a class of small organic molecules used as tool compounds in programmed cell death research (not a structural specification for this item).
• Exact ring systems, stereochemistry, and functional groups for the specific lot/formulation are not provided in this listing. Consult the product CoA or SDS for definitive identifiers and structural representation.

Notes:

• Where exact structural identifiers (SMILES/InChIKey) are required for cheminformatics or registration, use the batch-specific CoA/SDS associated with this SKU.

Synthetic Utility

Not typically applicable. This product is supplied as a finished small-molecule tool compound rather than a synthetic reagent or building block.

Guidance for chemists (general):

• If derivatization or analog synthesis is desired, consult primary literature on necrostatin chemotypes for reported scaffolds, points of diversification, and SAR trends. Use commercially available building blocks to recreate or modify the scaffold rather than repurposing this finished compound.
• For analytical support in custom synthesis efforts, employ HPLC/UPLC, NMR, and HRMS to match or surpass assay purity parameters documented for reference standards.

No named organic transformations are recommended using this compound as a reagent.

Target Specificity

No item-specific target specificity data are provided in this listing.

• Target name, affinity, selectivity panel: Not specified for this item; refer to CoA/Spec Sheet and primary literature.
• Note: While necrostatin-class compounds are widely used as pathway probes in regulated necrosis research (literature), do not infer target potency/selectivity for this specific item without consulting the batch documentation and peer-reviewed sources.

Foire aux questions

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 852391-19-6, the molecular formula is C13H12ClN3O2, and the molecular weight is 277.7 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.