NQDI 1 - ≥97% , CAS No.175026-96-7

CAS: 175026-96-7 Cat. No.: N288303 Formule: C₁₉H₁₃NO₄ Poids moléculaire: 319.31

GRADE & PURITY ≥97%

Synonyms

2,7-Dihydro-2,7-dioxo-3H-naphtho[1,2,3-de]quinoline-1-carboxylic acid ethyl ester | ethyl 2,7-dioxo-3,7-dihydro-2H-naphtho[1,2,3-de]quinoline-1-carboxylate | NQDI-1

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Vue d’ensemble

Product Application：

NQDI-1 was used to study LPS-induced activation and role of ASK1 and p38 in anti-inflammatory cytokine production by macrophages.

Specifications

Synonymes

2,7-Dihydro-2,7-dioxo-3H-naphtho[1,2,3-de]quinoline-1-carboxylic acid ethyl ester | ethyl 2,7-dioxo-3,7-dihydro-2H-naphtho[1,2,3-de]quinoline-1-carboxylate | NQDI-1

Spécifications et pureté

≥97%

Mécanismes biochimiques et physiologiques

Selective inhibitor of apoptosis signal-regulating kinase 1 (ASK1, MAP3K5) (IC50= 3μM, Ki= 500 nM).NQDI-1 is an inhibitor of apoptosis signal-regulating kinase (ASK1), Ki = 500 nM. NQDI-1 shows little or no inhibitory activity against Aurora A or ROCK kin

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Pureté

≥97%

Noms et identifiants

Pubchem Sid

504763903

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/504763903

Sourires canoniques

CCOC(=O)C1=C2C3=CC=CC=C3C(=O)C4=C2C(=CC=C4)NC1=O

IUPAC Name

ethyl 8,15-dioxo-14-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1(16),2,4,6,9(17),10,12-heptaene-16-carboxylate

InChIKey

UFJGFNHRMPMALC-UHFFFAOYSA-N

INCHI

1S/C19H13NO4/c1-2-24-19(23)16-15-10-6-3-4-7-11(10)17(21)12-8-5-9-13(14(12)15)20-18(16)22/h3-9H,2H2,1H3,(H,20,22)

Isomères SMILES

CCOC(=O)C1=C2C3=CC=CC=C3C(=O)C4=C2C(=CC=C4)NC1=O

Poids moléculaire

319.31

Reaxy-Rn

8932766

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=8932766&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Classe Anthracenes

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Classe: Anthracenes
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Anthracenes
• Alternative Parents: Isoquinolones and derivatives Hydroquinolones Hydroquinolines Pyridinecarboxylic acids Aryl ketones Pyridinones Vinylogous amides Heteroaromatic compounds Lactams Carboxylic acid esters Azacyclic compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Anthracene - Dihydroquinolone - Isoquinolone - Dihydroquinoline - Quinoline - Pyridine carboxylic acid - Pyridine carboxylic acid or derivatives - Aryl ketone - Pyridinone - Pyridine - Vinylogous amide - Heteroaromatic compound - Carboxylic acid ester - Lactam - Ketone - Azacycle - Organoheterocyclic compound - Carboxylic acid derivative - Organic nitrogen compound - Hydrocarbon derivative - Organic oxide - Organic oxygen compound - Organonitrogen compound - Organooxygen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as anthracenes. These are organic compounds containing a system of three linearly fused benzene rings.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Cibles associées (humaines)

FGFR1 Tclin Fibroblast growth factor receptor 1 (9149 Activities)

Discover Target: FGFR1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

ROCK1 Tclin Rho-associated protein kinase 1 (4723 Activities)

Discover Target: ROCK1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAPK10 Tchem c-Jun N-terminal kinase 3 (3396 Activities)

Discover Target: MAPK10

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MET Tclin Hepatocyte growth factor receptor (10718 Activities)

Discover Target: MET

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GLP1R Tclin Glucagon-like peptide 1 receptor (111429 Activities)

Discover Target: GLP1R

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PIN1 Tchem Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (36611 Activities)

Discover Target: PIN1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

TEK Tclin Tyrosine-protein kinase TIE-2 (3348 Activities)

Discover Target: TEK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

AURKA Tchem Serine/threonine-protein kinase Aurora-A (10240 Activities)

Discover Target: AURKA

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MAP3K5 Tchem Mitogen-activated protein kinase kinase kinase 5 (1965 Activities)

Discover Target: MAP3K5

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

POLI Tchem DNA polymerase iota (116820 Activities)

Discover Target: POLI

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

POLH Tchem DNA polymerase eta (21678 Activities)

Discover Target: POLH

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EHMT2 Tchem Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 (93046 Activities)

Discover Target: EHMT2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Cibles associées (non humaines)

Hdac6 Histone deacetylase 6 (222 Activities)

Discover Target: Hdac6

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Plasmodium falciparum (966862 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

rep Replicase polyprotein 1ab (378 Activities)

Discover Target: rep

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Propriétés chimiques et physiques

• Solubilité: Solvent:DMSO, Max Conc. mg/mL: 3.19, Max Conc. mM: 10
• Point de fusion (°C): >270°C
• Poids moléculaire: 319.300 g/mol
• XLogP3: 2.500
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 3
• Exact Mass: 319.084 Da
• Monoisotopic Mass: 319.084 Da
• Topological Polar Surface Area: 72.500 Ų
• Heavy Atom Count: 24
• Formal Charge: 0
• Complexity: 623.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Application Protocols

No vendor-validated application protocols are provided for this item.

General research workflow suggestions (non-validated; adapt to your system):

• Stock preparation: Dissolve in dry DMSO to a convenient concentration, filter-sterilize if required (0.22 μm PTFE), and aliquot into low-bind tubes.
• Dosing: Add to assays by serial dilution to minimize solvent effects. Include vehicle-only controls and, where appropriate, positive/negative controls for the biological pathway of interest.
• Stability checks: Monitor for precipitation or degradation in your assay matrix over the experimental timeframe using LC‑UV or LC‑MS.
• Documentation: Record lot number, stock concentration, solvent, preparation date, and storage conditions for reproducibility.

Note: These are general best practices for small-molecule research tools and are not validated for NQDI 1 by the manufacturer.

Biological Roles

Biological context (literature/general): NQDI 1 is referenced in the research literature as a small-molecule tool compound used to probe kinase-mediated signaling cascades in mammalian cells. Investigators employ such molecules to modulate upstream stress-response pathways and analyze downstream MAPK activation states in biochemical and cell-based assays. These uses are strictly in vitro/in vivo research contexts and do not imply suitability for diagnostic or therapeutic applications.

Considerations for pathway studies (general):

• Target engagement: Validate pathway modulation with orthogonal readouts (e.g., phospho-protein immunoblotting, kinase activity assays, or reporter assays) and include vehicle controls to account for solvent effects.
• Selectivity profiling: Literature often reports activity against a primary kinase target along with off-target panels. Confirm the latest peer-reviewed data for NQDI 1 to understand selectivity and concentration windows appropriate to your model system. Treat literature values as guidance, not product specifications.
• Kinetics and reversibility: Verify onset and washout behavior in your system. Adsorptive losses to plastics and serum binding can alter apparent potency.
• Cytotoxicity counter-screens: Determine non-specific toxicity thresholds in the relevant cell type to set maximum working concentrations.

Important: The product is provided “For research use only” and is not intended for human or veterinary use. No clinical, diagnostic, or therapeutic claims are made or implied.

Buffer Applications

This item is a discrete small-molecule research compound and is not a buffering agent. Buffer formulation guidance (pKa selection, ionic strength, Good’s buffers) is not typically applicable.

Practical notes for assay buffers (general):

• Vehicle compatibility: If preparing aqueous working solutions, introduce the compound from a concentrated DMSO or ethanol stock into pre-made assay buffer with vigorous mixing to avoid local supersaturation and precipitation.
• Adsorption: Hydrophobic small molecules can adsorb to plastics; precondition tips/tubes with buffer containing carrier protein or surfactant if compatible with your assay.
• Stability: Assess compound stability across the buffer pH range used in your assay, and avoid prolonged exposure to extreme pH or high temperatures.

Green Alternatives

Green chemistry comparisons are most meaningful for solvents, bulk reagents, or process chemicals. NQDI 1 is a discrete bioactive small molecule used at micro- to milligram scales in discovery research; there is typically no “green substitute” for the compound itself.

• Context: Green alternatives are not applicable to the identity of a specific probe compound. Instead, apply green principles to how you handle and formulate the material.

Greener handling strategies (general):

• Solvent choice: Where feasible, favor lower-toxicity diluents (e.g., ethanol or aqueous buffers) over high-toxicity aprotics for working solutions, provided solubility and assay performance are maintained.
• Minimize waste: Use microscale stock solutions, low-dead-volume vials, and careful planning to reduce disposal volumes of DMSO/DMF.
• Energy efficiency: Store only as cold as required (−20°C per Product Data) and avoid unnecessary freezer cycling. Shipments already use cold-chain packaging; consolidate orders to reduce packaging waste where possible.
• Safer analytics: Prefer LC‑UV or LC‑MS with aqueous-rich mobile phases and reduced acetonitrile usage when method robustness allows.

Trade-offs: Substituting greener co-solvents may reduce solubility or stability for certain heteroaromatics. Always confirm assay performance after any change in solvent or vehicle composition.

Pharmaceutical Uses

NQDI 1 is supplied strictly for research use only. It is not formulated or qualified as an excipient, active pharmaceutical ingredient (API), or for any clinical application.

Context for formulation scientists (general):

• Preformulation-like studies (for research): If your work involves in vitro models, you may characterize solubility in biorelevant media, stability vs. pH, and protein binding to guide dosing in cell-based assays. Treat all findings as research data, not as development specifications.
• Regulatory status: No pharmacopeial monograph or compendial status is provided for this item. Do not use in manufacturing or clinical settings.
• Handling aids: If low aqueous solubility is observed, research-grade solubilization approaches (cyclodextrins, lipid carriers, co-solvents) can be explored strictly within laboratory research contexts, ensuring compatibility with biological assays.

Always comply with institutional policies for non-clinical research materials, and do not extrapolate research observations to therapeutic claims.

Physical Properties

Item-specific physicochemical constants are not provided in the current listing; defer to the CoA/Spec Sheet and SDS for authoritative values.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet (literature values may exist; do not treat literature as product specification).
• Boiling point: Not applicable for most solid bioactive small molecules at ambient pressure; specific value not provided for this item.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Item-specific solubility data are not provided. In general, many bioactive heteroaromatic small molecules are soluble in polar aprotic organic solvents (e.g., DMSO) and may show limited aqueous solubility without co-solvent; confirm experimentally for this lot (general literature guidance; not a specification).
• logP/logD, pKa, refractive index: Not specified for this item; consult primary literature or predictive calculations if required for modeling (treat computed values as guidance only).

Practical notes (general):

• If preparing concentrated DMSO stocks, verify clarity and absence of visible particulates. For aqueous working solutions, use stepwise dilution into buffered media with surfactant (e.g., 0.1% Tween‑20) if compatible with your assay, or include co-solvent ≤1–2% v/v DMSO to maintain solubility. Filter through 0.22 μm if necessary.
• Assess stability in your chosen medium (temperature, pH, light). Where photolability is a concern for conjugated systems, protect from light.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet. For bioactive screening compounds, vendors typically provide HPLC purity and identity confirmation (LC‑MS/HRMS, NMR). Only values present on the CoA apply to this lot.

What grade means in practice (general guidance):

• Screening/library grade: Commonly emphasizes high chemical purity and identity suitable for biochemical/cellular assays. Low levels of residual solvents and synthesis by‑products are important for reproducibility. UV cutoffs, water/peroxide/metal limits are typically not defined unless the compound is sold as a solvent or reagent—consult CoA.
• Stabilizers: Not specified for this item; refer to CoA/Spec Sheet. If none are listed, assume neat material. If a stabilizer is present, evaluate its assay compatibility.
• Documentation: For regulated or GLP-adjacent workflows, retain the lot-specific CoA, SDS, and shipment records (temperature, packaging) to document chain-of-custody and storage.

Analytical expectations (general):

• Purity by HPLC/UPLC at a specified wavelength with method details (column, gradient, detection) is standard. Orthogonal purity (NMR integral balance) can be useful for compounds with weak chromophores.
• Identity by MS (m/z of [M+H]+/[M–H]−) and, where practical, 1H NMR with key diagnostic signals. If the target is chiral or stereo-defined, confirm enantiopurity/geometry if relevant to your assays.

Reaction and Applications

This product is supplied as a bioactive small molecule for research use and is not typically employed as a synthetic reagent, catalyst, or solvent. As such, classical reaction applications (e.g., cross‑couplings, Grignard chemistry, reductions) are not the primary use-case.

Research application context (general, literature-based):

• Small-molecule pathway probes like NQDI 1 are commonly used in biochemical and cell-based assays to interrogate signaling cascades. Ensure compatibility of the vehicle (e.g., DMSO) with your assay system and verify compound stability over the assay timeframe.
• For high-throughput screening or chemogenomic workflows, prepare single-use aliquots to minimize freeze–thaw and adsorption losses. Validate activity with positive/negative controls and, where relevant, counter-screens to exclude off-target or assay-interfering effects (e.g., redox cycling, fluorescence interference).

Analytical support:

• LC‑MS/MS methods can quantify compound levels in assay matrices to correlate exposure with observed biological effects.
• If performing structure–activity relationship (SAR) studies, maintain meticulous records of lot numbers and purity for reproducibility.

If you intend to use this molecule in synthesis (less common), treat it as a complex heteroaromatic substrate and assess functional group compatibility with your reaction conditions; protect sensitive motifs if necessary. However, no item-specific reactivity data are provided in this listing.

Reaction Conditions

Not typically applicable. NQDI 1 is a finished small-molecule probe supplied for research use, not a reagent designed for use under defined synthetic reaction conditions.

If derivative synthesis is contemplated (general guidance):

• Solvents: Polar aprotic media (e.g., DMF, DMSO, NMP, MeCN) are commonly used for functionalization of heteroaromatics; ensure anhydrous conditions if moisture sensitivity is suspected.
• Temperatures: Many heteroaromatic substitutions proceed from ambient to 120°C depending on the transformation (e.g., SNAr, amide couplings). Start with mild conditions and scale conservatively.
• Catalysts/reagents: For C–N/C–C couplings, Pd or Cu catalysts with appropriate ligands may be applicable; evaluate compatibility with heteroatoms that can chelate metal centers.
• Workup/yields: Expect that highly functionalized scaffolds can give modest to moderate yields with challenging separations; plan for RP‑HPLC purification when needed.

These are general literature practices and are not specifications or recommendations for this particular item.

Safety and Handling

Safety information specific to this item has not been provided in the listing. Always consult the product SDS for definitive hazard classification and response measures.

• GHS classification: Not specified for this item; refer to SDS.
• Signal word / H‑statements / Pictograms: Not specified for this item; refer to SDS.

General laboratory precautions for small-molecule research compounds:

• PPE: Wear lab coat, safety glasses, and suitable chemically resistant gloves (e.g., nitrile). Work in a chemical fume hood to avoid inhalation of dust or vapors.
• Avoid generating dust and avoid contact with skin, eyes, and clothing. Wash thoroughly after handling.
• Storage incompatibilities: Keep away from strong oxidizers and strong acids/bases unless compatibility is known. Segregate from reactive reducing/oxidizing agents as a precaution.
• Thermal/light sensitivity: Because many bioactive heteroaromatics can be light and/or heat sensitive, minimize exposure to light and store as directed (see Storage & Reconstitution). Use amber vials where appropriate.
• First aid (overview; defer to SDS): If inhaled—move to fresh air. Skin contact—wash with plenty of water. Eye contact—rinse cautiously with water for several minutes and remove contact lenses if present. Ingestion—rinse mouth; seek medical attention. Provide SDS to responders.
• Spill response: Avoid breathing dust; collect mechanically or damp-wipe. Dispose in accordance with institutional and local regulations.
• Waste: Treat as hazardous organic chemical waste; do not discharge to drains.

Solvent Selection

Item-specific solvent compatibility is not provided. The guidance below reflects common practice for small-molecule bioactive compounds and should be verified experimentally for NQDI 1 (lot-specific behavior may vary).

• Primary stock solvent (literature/general): Dimethyl sulfoxide (DMSO) is widely used to prepare concentrated stocks (e.g., millimolar range) for dispensing into aqueous assay media. Confirm solubility and stability in DMSO for this item before preparing large stocks.
• Aqueous work solutions: Prepare by serial dilution of the DMSO stock into buffered media with gentle mixing to minimize precipitation. Keep final DMSO typically ≤0.1–1% v/v for cell-based assays, as assay tolerance allows (general practice; not product-specific guidance).
• Alternative organic solvents (general): DMF, ethanol, or acetonitrile may dissolve certain heteroaromatics, but consider assay compatibility, evaporation rate, and toxicity profiles.
• Polarity and miscibility: Without an item-specific structure, treat as an organic small molecule likely favoring polar aprotic solvents. Always check for visible precipitation after dilution and consider 0.22 μm filtration for clarity.

Comparison (general):

• DMSO: Highest solvating power for diverse heteroaromatics; hygroscopic; can affect some biological readouts at higher %.
• Ethanol: Better biocompatibility at higher % than DMSO; lower solvating power for some hydrophobic scaffolds.
• DMF/MeCN: Good solvents for analytics and synthesis; evaluate biological compatibility before use.

Storage and Reconstitution

• Storage conditions (from Product Data): Store at −20°C. Shipments are supplied in an ice chest with ice pads to maintain a cold chain during transit.
• Container: Keep tightly closed in the original container. Use desiccation and light protection (e.g., amber vial) if the compound is light/moisture sensitive (item-specific sensitivity not provided; adopt as a precaution).

Reconstitution (general guidance for small-molecule probes; not product specification):

• Solvent: DMSO is commonly used to prepare concentrated stock solutions of heteroaromatic bioactive compounds. Verify solubility for this item on a small scale before preparing working stocks.
• Technique: Allow the vial to equilibrate to room temperature in a desiccator before opening to avoid moisture condensation. Add solvent with a calibrated microliter syringe, vortex, and, if needed, sonicate briefly. Filter (0.22 μm PTFE) if particulates persist.
• Aliquoting: Prepare single-use aliquots in low-bind, amber tubes to minimize freeze–thaw cycles and photodegradation. Label with concentration, solvent, and date.
• Working solutions: Dilute stocks into assay buffer/media immediately before use. Maintain final organic solvent content at levels tolerated by your system.

Stability considerations (general):

• Avoid repeated freeze–thaw. Store aliquots at −20°C (short- to mid-term) and protect from light. For extended storage, monitor purity periodically by HPLC/LC‑MS. Discard solutions that show precipitation, discoloration, or significant degradation.

Research Use Only: Not for human or veterinary use.

Structure and Identity

High-purity small-molecule research compound supplied by Aladdin Scientific under the catalog name NQDI 1 (SKU: N288303), intended for research use only.

• CAS: 175026-96-7
• PubChem CID (from Product Data): 5522952
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural description (general guidance):

• Item-specific structural features (ring systems, heteroatoms, stereochemistry) are not provided in the current listing. Consult the Certificate of Analysis (CoA) or technical data sheet for definitive structural identifiers and spectral confirmation (1H/13C NMR, HRMS, HPLC/UPLC purity, and, if applicable, stereochemical assignments).
• For referencing and literature correlation, use the CAS and PubChem CID above to locate peer-reviewed structure depictions and spectral datasets (literature values; not product specifications).

Identity confirmation practices (general):

• Recommended orthogonal checks include HPLC or UPLC retention time vs. reference, MS molecular ion, and diagnostic fragment ions. Where available, compare NMR (DMSO‑d6 or CDCl3, as appropriate) with literature spectra. IR can corroborate major functional groups.
• If your application is target/pathway research, verify batch identity and purity prior to biological assays to ensure reproducibility and accurate dose–response interpretation.

Synthetic Utility

This product is offered as a bioactive research compound rather than as a general synthetic building block. Consequently, it is not typically used as a reagent or intermediate in routine organic synthesis.

If used in synthesis (uncommon; general guidance):

• Functional group tolerance: Complex heteroaromatic scaffolds often contain basic or hydrogen-bonding motifs that can interact with catalysts or acids/bases. Test small-scale reactions to evaluate compatibility.
• Protection strategies: If nucleophilic heteroatoms are present (e.g., amines), transient protection might be required under acylation, alkylation, or metal-catalyzed coupling conditions.
• Purification: Bioactive small molecules may exhibit strong adsorption on silica. Reverse-phase preparative HPLC can be preferable for final purification.
• Analytics: Use LC‑MS to track transformations and detect closely related impurities. NMR in DMSO‑d6 or CD3OD can aid in characterizing polar heteroaromatics.

Note: No item-specific reactive handles, named-reaction suitability, or derivatization routes are provided in the Product Data. Consult primary literature associated with the CAS or CID for any published semisynthetic modifications if such work is relevant to your research.

Target Specificity

Target- or epitope-specific details are not provided in the Product Data for this item.

• Target name, binding site/epitope, species selectivity, clone/isotype (if antibody), and assay-validated activities: Not specified for this item; refer to primary literature associated with CAS 175026-96-7 or PubChem CID 5522952 for general research context. Treat any literature values solely as informational and not as product specifications.