Nutrient Broth Medium (NB) - BioReagent, Suitable for microbiology

Cat. No.: N1523355

QUALITÉ & PURETÉ BioReagent, Suitable for microbiology

Synonyms

Nutrient Broth | NB

Storage

Protected from light,Room temperature,Desiccated

Shipped In

Normal

Vue d’ensemble

Nutrient Broth Medium (NB) is used for the cultivation, sub‑culturing, rejuvenation and enrichment of common bacteria.

Principle and Purpose: Peptone and beef extract powder supply essential nutrients for bacterial growth, including nitrogen sources, vitamins, minerals and amino acids. Sodium chloride maintains balanced osmotic pressure.

Preparation Method: Weigh 18.0 g of this product and dissolve it in 1 L of purified water. Heat and stir until completely dissolved, dispense into containers, autoclave at 115 °C for 30 min, cool and set aside for later use.

Scope of Application:

Application	Category
Enrichment	Mesophilic aerobic bacteria

Formula (g/L):

Peptone	10.0g
Beef Extract Powder	3.0g
Sodium Chloride	5.0g
Final pH (25℃)	7.2±0.2

Quality Control:

Test Item	Specification
Appearance	Free-flowing powder
Colour of dehydrated medium	Pale yellow
Colour of prepared medium	Pale yellow clear liquid
Final pH (25 °C)	7.2±0.2

Microbiological Testing:

Test for Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Salmonella paratyphi‑B in accordance with the Pharmacopoeia of the People's Republic of China.

QC Strain and Strain No.	Growth Performance / Characteristics
Staphylococcus aureus CMCC(B)26003	Good growth, turbidity
Pseudomonas aeruginosa CMCC(B)10104	Good growth, turbidity
Bacillus subtilis CMCC(B)63501	Good growth, turbidity, with a small amount of wrinkled pellicle on the liquid surface.
Escherichia coli CMCC(B)44102	Good growth, turbidity
Salmonella Paratyphi‑B CMCC(B)50094	Good growth, turbidity

Inoculation level: 10-100CFU

Incubation conditions: 35°C for 24 hours

Reference Standard:

Chinese Pharmacopoeia

Precautions:

1. Take necessary protective measures when weighing the medium to avoid physical discomfort.

2. The medium shall be stored in a dark and dry place. Tighten the bottle cap immediately after use to prevent moisture absorption and caking.

3. The used medium shall be autoclaved at 121℃ for 30 minutes before disposal.

4. This product is not intended for clinical testing.

Spécifications

Synonymes

Nutrient Broth | NB

Spécifications et pureté

BioReagent, Suitable for microbiology

Stabilité et stockage

Store at room temperature long term (36 months). Store in the dark. Keep dry.

Conditions de stockage de stockage

Protected from light,Room temperature,Desiccated

Expédié en

Normal

Grade

BioReagent, Suitable for microbiology

Product Line

Produits biologiques

Noms et identifiants

PH

7.0-7.4

Propriétés chimiques et physiques

• Sensibilité: Light-sensitive

Application Protocols

General protocol guidance for reconstituting and using Nutrient Broth (literature-based; not item-specific specifications):

• Preparation (1 L example):
  • Weigh the manufacturer-recommended amount of dehydrated powder (typical literature: 8–13 g).
  • Add to ~900 mL purified water with stirring until dissolved; adjust volume to 1 L.
  • Optional: Adjust pH to ~7.0 at 25 °C if required by method.
  • Dispense into suitable vessels with headspace for aeration.
  • Sterilize by autoclaving (e.g., 121 °C, 15 min). Allow to cool before use.
• Inoculation and incubation:
  • Inoculate aseptically with the test organism or sample.
  • Incubate at organism-appropriate temperature and agitation (e.g., 30–37 °C; 150–250 rpm for aerobes).
  • Monitor growth by turbidity/OD600 or plating as required.
• Supplements (if needed): Add sterile carbohydrates, antibiotics, or indicators post-sterilization as dictated by your assay (note: additives can change selectivity).
• Disposal: Autoclave spent cultures and disposables before discarding per institutional biosafety procedures.

For this SKU, exact preparation mass, pH, and growth performance criteria are Not specified for this item; refer to CoA/Spec Sheet and product label.

Biological Roles

Nutrient Broth provides the macronutrients and micronutrients necessary to support heterotrophic microbial growth in laboratory settings. It functions as a balanced source of carbon, nitrogen, vitamins, and minerals.

• Carbon and energy: Carbohydrates and complex organics in extracts serve as readily metabolizable carbon sources for many bacteria.
• Nitrogen: Peptones supply amino acids and short peptides for rapid assimilation into cellular proteins and metabolic pathways.
• Vitamins/growth factors: Yeast or beef extracts typically provide B-complex vitamins, nucleotides, and trace growth factors that stimulate robust growth of nonfastidious organisms.
• Ionic balance: Added salts (e.g., NaCl in many formulations) maintain osmotic pressure and help stabilize enzyme function.
• Non-selective profile: The lack of inhibitory dyes, antibiotics, or extreme salt/sugar levels allows broad-range cultivation, making it suitable for enrichment prior to selective steps.

Use contexts (general):

• Growth-curve analysis and physiological studies (e.g., temperature/pH dependence, oxygen demand) under controlled shaking/static conditions.
• Biomass generation for downstream applications such as plasmid extraction (in appropriate host strains), enzyme expression pre-cultures, or preparation for transformation/selection in defined media.

Note: Exact composition and growth performance parameters for this item are Not specified for this item; consult CoA/Spec Sheet for organism panels and acceptance criteria if provided.

Buffer Applications

Nutrient Broth is not primarily a buffering system. Standard formulations may exhibit modest intrinsic buffering capacity from amino acids and phosphates present in extracts, but they are not optimized buffers.

• If a defined buffering range is required (e.g., for enzyme assays or pH-sensitive organisms), consider using a buffered medium (e.g., phosphate-buffered peptone water) or adding a suitable biological buffer (HEPES, MOPS) post-sterilization.
• Practical tip: Monitor and adjust pH of reconstituted broth before sterilization if your protocol requires a specific starting pH. Exact pH specifications for this item are Not specified for this item; refer to CoA/Spec Sheet and product label.

Green Alternatives

Environmental considerations for culture media focus on source materials, packaging, and sterilization energy rather than solvent selection.

Greener practice options (general):

• Plant-based peptones/yeast extract can reduce reliance on animal-derived materials. Verify performance for your strains before full adoption.
• Right-size preparation: make only the volume needed to reduce autoclave energy and waste from expired media.
• Sterilization efficiency: consolidate autoclave loads, use validated cycles, and consider membrane filtration for small volumes and heat-labile mixtures to reduce energy use.
• Packaging: select bulk packaging to reduce plastic, or recyclable containers where available; decant to working bottles as needed.

Comparison (general literature):

| Option | Sustainability benefit | Trade-offs | |---|---|---| | Plant peptone nutrient broth | Reduced animal inputs | Possible growth differences vs animal peptones | | Yeast-extract–based media | Byproduct valorization | Batch-to-batch variability | | Ready-to-use liquid media | Convenience; reduced dust | Higher shipping weight; more plastic waste | | Dehydrated powder (this format) | Lower weight/volume, reduced shipping emissions | Requires on-site sterilization energy |

Note: No item-specific sustainability claims are made for this SKU; practices above are general recommendations.

Pharmaceutical Uses

No clinical or therapeutic claims are made. In research and industrial laboratory settings, Nutrient Broth can support process development and quality control tasks that involve microbial cultivation.

Typical laboratory/industrial roles (general):

• Non-selective growth/recovery medium for environmental monitoring isolates or equipment/utility bioburden studies (method- and facility-dependent).
• In-process testing in fermentation R&D to maintain seed cultures prior to transfer into production media.
• Media fill simulations (broth trials) use specialized media; while nutrient-type broths can be employed for practice runs, validated fills generally specify defined media—follow your quality system. This product is not qualified for GMP without user validation.
• Assay reagent base where microbial growth is an indicator (e.g., disinfectant screening), following relevant compendial or in-house methods.

Compliance note:

• Pharmacopeial designations, compendial conformity, endotoxin/bioburden limits, and growth promotion testing requirements are application- and supplier-specific. For this SKU, such specifications are Not specified for this item; refer to CoA/Spec Sheet. User validation is required for regulated workflows.

Physical Properties

Item-specific specifications are not provided for this product; consult the product label and CoA/SDS for definitive values.

• Appearance (item-specific): Not specified for this item; refer to CoA/Spec Sheet. (Typical literature: free-flowing tan powder or granules.)
• Solubility (literature): Freely soluble in water; forms a clear to slightly opalescent solution after dissolution and sterilization. Not soluble in nonpolar organic solvents.
• pH after reconstitution (literature): Often in the range pH ~6.8–7.4 at 25 °C for standard formulations; exact pH depends on brand/lot and concentration. Not specified for this item; refer to CoA/Spec Sheet.
• Concentration for use (literature): Commonly 8–13 g/L for standard nutrient broth; exact directions vary by manufacturer. Not specified for this item; refer to product label.
• Osmolality (literature): Dependent on formulation and concentration; typically low to moderate ionic strength.
• Sterility: Product is supplied non-sterile (typical for dehydrated media); final media should be sterilized by autoclaving or filtration as appropriate. Not specified for this item; refer to CoA/Spec Sheet.

Note: As a complex mixture, discrete thermophysical constants (bp, mp, density, refractive index, logP, pKa) are not applicable in the usual sense. Prepare and characterize reconstituted broth according to laboratory QC practices (e.g., pH check, clarity, and growth promotion testing) where required.

Quality and Grades

• Item-specific grade: BioReagent — indicates suitability for biological research applications, with controlled bioburden and composition consistent with use as a culture medium component. It does not imply clinical/diagnostic use.
• Quality attributes (general for microbiological media):
  • Growth promotion characteristics against defined QC organisms (e.g., E. coli, S. aureus) — typically verified by manufacturers; check CoA for organism list and acceptance criteria.
  • Lot-to-lot consistency of nitrogen sources (peptones) and extract composition.
  • Low background inhibition (absence of toxic residues, heavy metals within acceptable ranges for microbial growth). Specific metal limits for this item: Not specified for this item; refer to CoA/Spec Sheet.
  • Moisture content and pH after reconstitution within labeled ranges (item-specific ranges: Not specified for this item).
• Stabilizers/antimicrobials: None are typically included in dehydrated nutrient broth powders; any additives would be listed on the product label. Not specified for this item; refer to CoA/Spec Sheet.
• Documentation: For this SKU, detailed specifications (e.g., growth promotion performance, recommended concentration, pH range) are Not specified for this item; refer to CoA/Spec Sheet.
• Use scope: For research and general laboratory microbiology. Not tested or qualified for clinical diagnostics, therapeutic, or food production without additional validation.

Reaction and Applications

As a culture medium, Nutrient Broth is used to support microbial growth for diverse laboratory workflows. While not a reagent in chemical reactions, it underpins many biological “process reactions” (growth, metabolism, enzyme expression).

Key applications (general, literature):

• Non-selective cultivation of bacteria from environmental, food, and laboratory sources prior to selective plating or biochemical testing.
• Starter/pre-cultures for inoculating richer or selective media, fermentation runs, or solid media preparation.
• Viable count enrichment before serial dilution and plating.
• QC of sterilization and disinfectant efficacy by providing a recovery medium for sublethally injured cells (method-dependent).
• Teaching laboratories for demonstrating growth curves, oxygen requirements (with/without agitation), and effects of temperature or pH.

Practical tips:

• Reconstitute at the manufacturer-recommended concentration (Not specified for this item; refer to label/CoA). Typical literature values are ~8–13 g/L.
• Sterilize by autoclaving (e.g., 121 °C, 15 min). For heat-labile supplements, filter-sterilize the supplement and add aseptically after cooling to ~45–50 °C.
• For aerobes, use baffled flasks and sufficient headspace (≥5:1 air:liquid) with agitation. For microaerophiles or facultative anaerobes, adjust agitation and vessel closure accordingly.
• Confirm performance for your organism via small-scale growth tests; complex media can vary by peptone source and lot.

Reaction Conditions

Interpreted as preparation and use conditions for culture media (general literature guidance). Item-specific directions are Not specified for this item; refer to product label/CoA.

• Reconstitution: Dissolve the recommended mass of dehydrated powder in purified water to the target volume (typical literature: ~8–13 g per liter). Mix until fully dissolved.
• pH adjustment: If required by protocol, adjust to ~7.0 at 25 °C with sterile 1 N HCl/NaOH. Exact target pH, if any, is manufacturer-dependent.
• Sterilization: Autoclave (e.g., 121 °C for 15 minutes). For heat-labile supplements, sterile-filter (0.22 µm) and add aseptically after cooling the autoclaved broth to ~45–50 °C.
• Incubation parameters: Temperature and time depend on organism (e.g., 25–37 °C for many mesophiles; shaking 150–250 rpm for aerobes in baffled flasks). Validate for your strain.
• Expected outcomes: Clear to slightly turbid media pre-inoculation; turbidity increase (OD600) correlates with growth. Growth promotion performance criteria are method-specific; not specified for this item.
• Quality checks: Verify pH and clarity after sterilization. Optional: perform growth promotion with reference strains to confirm lot suitability.

Note: Do not interpret these as product specifications; they are standard microbiology practices provided for planning purposes.

Safety and Handling

Always consult the SDS for authoritative safety information. The following are general guidelines for dehydrated culture media.

• GHS/SDS details (item-specific): Signal word, H-Statements, GHS classification, pictograms — Not specified for this item; refer to SDS.
• Primary hazards (general): Low inherent chemical hazard anticipated for typical nutrient broths; dust may cause mechanical irritation to eyes/respiratory tract. Avoid inhalation of dust and contact with eyes.
• PPE (general best practice): Laboratory coat, safety glasses or splash goggles, disposable gloves (e.g., nitrile). Use a dust mask or work in a fume hood/biological safety cabinet when handling powders to minimize aerosol generation.
• Incompatibilities (general): Strong oxidizers may degrade organic components. Avoid moisture uptake; store tightly closed. Do not mix with biocides unless intended.
• Microbiological safety: Once reconstituted and inoculated, cultures can contain viable microorganisms. Handle using appropriate biosafety level (BSL) practices for the organism. Decontaminate spent media and materials by autoclaving (e.g., 121 °C, 15 psi, 15–30 min) or approved chemical disinfectants.
• First aid (general): Eye contact—rinse with water for several minutes; remove contact lenses if present. Skin contact—wash with soap and water. Inhalation—move to fresh air. Ingestion—rinse mouth. Seek medical attention if symptoms persist.
• Spill response: Avoid dust generation; sweep or vacuum with HEPA filtration. For liquid media, absorb with inert material and disinfect.
• Waste disposal: Treat as laboratory waste; autoclave biologically contaminated materials prior to disposal in accordance with institutional and local regulations.

Solvent Selection

This product is a dehydrated microbiological medium intended for reconstitution in water; organic solvent selection is not typically applicable.

• Primary solvent (applicable): Purified water (e.g., distilled or deionized). Sterile water is used when sterile filtration is the terminal sterilization step.
• Miscibility (general): Readily dissolves/disperses in water with stirring and gentle heat if needed. Not designed for dissolution in organic solvents.
• Practical guidance:
  • Use high-purity water to minimize inhibitory ions or residual disinfectants (e.g., chlorine, chloramines).
  • For downstream applications requiring low background fluorescence/absorbance, pre-test water source and sterilization method.
  • Avoid hard water; divalent cations may alter performance.
• When to consider alternatives: If selective or buffered conditions are needed, choose a different formulation (e.g., buffered peptone water, tryptic soy broth, LB broth) rather than changing the solvent.

Storage and Reconstitution

• Storage conditions (item-specific): Room temperature. Keep container tightly closed in a dry place to prevent moisture uptake and caking. Protect from strong odors and contaminants.
• Shipping (item-specific): Shipped under normal conditions.
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet and product label. Use by the indicated date for best performance.
• Reconstitution (general literature guidance):
  • Dissolve the recommended amount of powder in purified water to the desired volume (typical literature: 8–13 g/L).
  • Mix until completely dissolved; adjust pH if required by your protocol.
  • Sterilize by autoclaving (e.g., 121 °C, 15 minutes) or filter-sterilize when appropriate. Cool before adding heat-labile supplements.
• Prepared media storage (general): Store sterile broth at 2–8 °C in tightly capped containers; use within a few weeks for best performance. Inspect for contamination, precipitation, or pH drift prior to use.
• Freeze–thaw guidance: Not typically frozen; if freezing aliquots for specialized workflows, validate performance after thaw. Avoid repeated freeze–thaw cycles.

Note: Exact reconstitution parameters, pH targets, and performance specifications for this SKU are Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

Nutrient Broth is a complex, undefined microbiological growth medium composed of hydrolysates and extracts; it is not a single chemical entity.

• Product name (SKU): Nutrient Broth (N1523355)
• Type: Complex, non-synthetic culture medium (microbiology)
• Grade/Purity (item-specific): BioReagent
• Research use note (item-specific): For research use only
• Chemical identifiers: CAS, CID, InChIKey, SMILES — Not applicable to complex mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula / Molecular weight: Not applicable to mixtures; not specified for this item; refer to CoA/Spec Sheet.

Structural features (general for nutrient broths, literature):

• Typically contains peptones (enzymatic digests of casein/gelatin or plant peptones), beef/yeast extracts, and electrolytes (e.g., NaCl). These components supply amino acids/peptides, vitamins, nucleotides, minerals, and osmotic balance.
• Being a heterogeneous blend of oligomeric peptides, amino acids, carbohydrates, and salts, it has no single defined 2D/3D structure or stereochemistry.

2D description in words (general):

• A mixture of small peptides and amino acids (amide functional groups), simple carbohydrates (polyhydroxy aldehydes/ketones), organic acids, and inorganic salts dispersed in water upon reconstitution.

Synthetic Utility

This product is a biological growth medium and is not used as a synthetic organic reagent or building block.

• For chemical synthesis planning, refer instead to defined reagents and solvents. Nutrient Broth does not provide predictable reactivity or stoichiometry due to its complex, undefined composition.
• If your workflow involves biocatalysis or whole-cell transformations, Nutrient Broth may serve as the growth phase medium to generate biomass prior to transferring cells into a defined reaction medium optimized for catalysis.
• No named organic reactions or protective group strategies apply to this product.

Target Specificity

Not applicable. This product is a general-purpose culture medium and is not an antibody, probe, or targeted binding reagent. No clone, isotype, antigen, or species reactivity data apply.