ODN 2007 - ≥93% , CAS No.455348-63-7

CAS: 455348-63-7 Cat. No.: O649220 Poids moléculaire: 6852.50

GRADE & PURITY ≥93%

Storage

Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Vue d’ensemble

ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor ( TLR ) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3' .

In Vitro

ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression. ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO. MCE has not independently confirmed the accuracy of these methods. They are for reference only. RT-PCRCell Line: MQ-NCSU cells (a chicken macrophage cell line) Concentration: 1 μg/mL, 5 μg/mL Incubation Time: 3 h, 12 h, 18 h Result: Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose. Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point. Western Blot AnalysisCell Line: HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line Concentration: 10 μg/mL Incubation Time: 0.5 h, 1 h, 3 h, 6 h, 12 h Result: Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels. Stimulated an increase in the level of NO.

In Vivo

ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected with Vibrio traumaticus FJ03-X2. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Zebrafish infected with Vibrio traumaticus FJ03-X2Dosage: 1 μg Administration: Intraperitoneal injection; once Result: Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.

Form:Solid

Specifications

Spécifications et pureté

≥93%

Mécanismes biochimiques et physiologiques

ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor ( TLR ) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCG

Conditions de stockage de stockage

Store at -20°C,Desiccated

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Type d'action

AGONIST

Pureté

≥93%

Noms et identifiants

• Poids moléculaire: 6852.50

Propriétés chimiques et physiques

• Solubilité: H2O : 20 mg/mL (2.92 mM; Need ultrasonic)

Foire aux questions

What is the purity of this product?

This product is supplied at ≥93% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 455348-63-7, and the molecular weight is 6852.50 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.