When 1-4mM MgCl2 is contained, the concentration of each dNTP is 200 μ M. Targeting λ Primers for about 500 base pair regions of DNA (concentration is 1.0 respectively μ M），1ng/100 μ L's λ DNA template and 2.5 units/100 μ In the reactant of Taq DNA polymerase L, 10X PCR buffer II was tested with 1X final concentration (10mM Tris HCl, pH 8.3, 25 ° C, 50mM Kcl). The reaction was carried out for 25 cycles, double stranded DNA was denatured at 94 ℃, DNA fragments were annealed at 55 ℃ and 72 ℃
Product application:
The 10x PCR buffer without MgCl2 has been used with PCR enzyme. The 10X PCR buffer without MgCl2 has been used as a component in the PCR amplification mixture for the amplification of mycotoxin mold DNA, parasite DNA and Fusarium oxysporum DNA.
Product specifications and parameters:
SKU
Name
Specification
Specification
Pack Size
P493004-A10×-1.5ml
PCR Buffer
without MgCl₂
10×
1.5ml
P493004-A10×-5ml
PCR Buffer
without MgCl₂
10×
5ml
P493004-B10×-5ml
PCR Buffer
MgCl₂ included
10×
5ml
P493004-B10×-5EA
PCR Buffer
MgCl₂ included
10×
5×1.5ml
P493004-B10×-25ml
PCR Buffer
MgCl₂ included
10×
25ml
P493004-B10×-1EA
PCR Buffer
MgCl₂ included
10×
1.5ml
Specifications
Conditions de stockage de stockage
Store at -20°C
Expédié en
Ice chest + Ice pads
Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Avis
Avis des clients
Foire aux questions
How should this product be stored?
Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
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