Phosphatase, Alkaline, CAS No.9001-78-9

CAS: 9001-78-9 Cat. No.: P128629 Numéro CE: 232-631-4 PubChem CID: 18985873

QUALITÉ & PURETÉ Native, EnzymoPure™, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Accession #

P00634

Bioactivity

≥30 units/mg protein (25°C, pH 8.0)

Vue d’ensemble

Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.

Unit Definitons

E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.

Spécifications

Product Name

Phosphatase, Alkaline, CAS No.9001-78-9

Synonymes

Alkaline phosphatase | Phosphatase, alkaline | ENTEROSTATIN,HUMAN | alkaline phosphomonoesterase | glycerophosphatase | phosphomonoesterase | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) | alkaline phosphatase | Apase, Escherichia coli |

Grade

Native, EnzymoPure™

Spécifications et pureté

EnzymoPure™, Nature, ≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Mécanismes biochimiques et physiologiques

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad

Bioactivité

≥30 units/mg protein (25°C, pH 8.0)

Accession #

P00634

CAS

9001-78-9

Numéro de commission de l'enzyme

I.U.B.: 3.1.3.1

Type de molécule

Enzyme

Stockage et expédition

Concentration

≥30 units/mg protein (25°C, pH 8.0),from Escherichia coli

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Définition de l'unité

One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0.