Phosphotungstic Acid Ethanol Solution (3%) - BioReagent, Biological Stain, for microscopy, 3%

Cat. No.: E1510324

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 3%

Synonyms

3% PTA Ethanol Solution | 3% Phosphotungstic Acid in Ethanol | Ethanolic Phosphotungstic Acid Solution (3%)

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Overview

  Negative staining, also known as negative contrast staining, is a staining technique discovered by Hall as opposed to conventional staining (i.e., positive staining). The principle involves surrounding low-electron-density samples with heavy metal salts to increase the electron density around the sample, thereby creating mass-thickness differences among fine structures and enhancing the scattering-absorption contrast. This allows the sample to appear as bright structures against a dark background. Common negative staining agents include phosphotungstic acid, ammonium molybdate, and India ink, among which 1–3% phosphotungstic acid is the most frequently used.

  Phosphotungstic Acid Ethanol Solution (3%) is primarily composed of phosphotungstic acid and ethanol. Phosphotungstic acid serves as the active component for staining sperm maturity and sperm nuclear histones. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other applications.

Specifications

Synonyms

3% PTA Ethanol Solution | 3% Phosphotungstic Acid in Ethanol | Ethanolic Phosphotungstic Acid Solution (3%)

Specifications & Purity

BioReagent, Biological Stain, for microscopy, 3%

Stability And Storage

Store at room temperature long term (12 months). Store in the dark.

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy

Chemical and Physical Properties

• Sensitivity: Light-sensitive