PMS2 Antibody - Primary antibody, specific to PMS2, Rabbit IgG

Cat. No.: Ab122454

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

IHC, WB

Host species

Rabbit

Reactive species

Human

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human PMS2 Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function:
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Specifications

Product Name

PMS2 Antibody - Primary antibody, specific to PMS2, Rabbit IgG

Synonyms

DNA mismatch repair gene homologueantibody | DNA mismatch repair protein PMS2 antibody | H_DJ0042M02.9 antibody | HNPCC4 antibody | Mismatch repair endonuclease PMS2 antibody | Mismatch repair gene PMSL2 antibody | MLH4 antibody | PMS 2 antibody | PMS1 ho

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Rabbit

Specificity

PMS2

Immunogen

A synthetic peptide derived from human PMS2 (AA 9-24)

Positive Control

WB: Jurkat, HeLa and SK-N-SH cell lysates. IHC: Human tonsil tissue.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PMS2 Antibody (Ab122454)

PMS2 Antibody (Ab122454) - Western Blot
All lanes: PMS2 Antibody (Ab122454) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 96 kDa
Observed band size: 48, 54, 107 kDa
Exposure time: 16.5 s

IHC: PMS2 Antibody (Ab122454)

PMS2 Antibody (Ab122454) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using PMS2 Antibody (Ab122454). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

Associated Targets(Human)

PMS2 Tbio Mismatch repair endonuclease PMS2 (0 Activities)

Discover Target: PMS2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SMARCA4 Tchem Transcription activator BRG1 (0 Activities)

Discover Target: SMARCA4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

SMARCA4 Tchem Transcription activator BRG1 (263 Activities)

Discover Target: SMARCA4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action