Product Introduction
Polyphenol Oxidase (PPO) is a metalloproteinase widely distributed in nature. It is generally present in the plastids of plants, fungi and insects, and PPO activity can even be detected in decaying plant residues in the soil. As a proteosome, PPO participates in a series of enzyme-catalyzed chemical reactions during the life activities of tea plants and the processing of tea leaves, hence it is also known as a biocatalyst. As a key enzyme involved in the intracellular lignin biosynthesis pathway, research on PPO can facilitate the exploration of metabolic mechanisms underlying lignin deposition during the developmental processes of various biological cells, providing a theoretical basis for reducing the content of stone cells in fruits and improving their quality.
The Polyphenol Oxidase (PPO) Extraction Reagent is primarily designed for lysing plant tissues and extracting PPO from samples. This reagent is intended for research use only and is not suitable for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Distilled water
2. Centrifuge tubes or test tubes, homogenizer or mortar and pestle, refrigerated centrifuge
Operating Procedures (For Reference Only)
1. Take fresh plant tissue, rinse it thoroughly, and cut it into small pieces.
2. Add the Polyphenol Oxidase (PPO) Extraction Reagent at a ratio of 3 g plant tissue: 6 ml reagent, then fully homogenize or grind the mixture under ice bath conditions.
3. Centrifuge at 10,000×g and 4°C for 10-15 minutes, and collect the supernatant.
4. Store the supernatant at 4°C for subsequent Polyphenol Oxidase (PPO) activity assays or other experimental applications.
Precautions
1. The sample to be tested must not contain phosphatase inhibitors, and repeated freeze-thaw cycles should be avoided.
2. If the measured value of the sample exceeds the upper limit of the standard curve, dilute the sample with the extraction reagent and perform the assay again.
3. Please use the reagent as soon as possible after opening to prevent it from affecting the results of subsequent experiments.
4. For your safety and health, wear a lab coat and disposable gloves during the operation.
ZJ26F0232128