PPP2R1A Antibody - Anticorps primaire, specific to PPP2R1A, Rabbit IgG

Cat. No.: Ab122840

GRADE & PURITY Carrier Free, ExactAb™, Validated, High Performance, 1.14 mg/mL

Application

IF/ICC, IHC, WB

Host species

Lapin

Reactive species

conseil, Humain, Souris

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human PPP2R1A Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/3000
IHC: 1/50-1/100
Protein Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis.

Specifications

Product Name

PPP2R1A Antibody - Anticorps primaire, specific to PPP2R1A, Rabbit IgG

Synonymes

2AAA_HUMAN antibody | 6330556D22Rik antibody | Alpha isoform of regulatory subunit A, protein phosphatase antibody | DKFZp470L0914 antibody | Medium tumor antigen-associated 61 kDa protein antibody | MGC128399 antibody | MGC786 antibody | MGC83000 antibod

Spécifications et pureté

ExactAb™, Validated, Carrier Free, High performance, 1.14 mg/mL

Espèce hôte

Lapin

Spécificité

PPP2R1A

Immunogène

Fusion protein of human PPP2R1A (AA 500-580)

Contrôle positif

WB: C6, Jurkat and HEK293 cell lysates. IHC: Human liver cancer lysates. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, High Performance, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.14 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PPP2R1A Antibody (Ab122840)

PPP2R1A Antibody (Ab122840) - Western Blot
All lanes: PPP2R1A Antibody (Ab122840) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 65 kDa
Observed band size: 60 kDa
Exposure time: 5.0 s

IHC: PPP2R1A Antibody (Ab122840)

PPP2R1A Antibody (Ab122840) - IHC
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PPP2R1A Antibody (Ab122840). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: PPP2R1A Antibody (Ab122840)

PPP2R1A Antibody (Ab122840) - IF/ICC

IF analysis of PPP2R1A Antibody (Ab122840) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PPP2R1A Antibody (Ab122840) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

IHC

1/50 - 1/100

WB

1/500-1/3000

IF/ICC

1/200

Cibles associées (humaines)

PPP2R1A Tbio Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (0 Activities)

Discover Target: PPP2R1A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PPP2R1A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.