Propidium iodide (PI) - ≥94% , CAS No.25535-16-4

CAS: 25535-16-4 Cat. No.: P113815 Formule: C₂₇H₃₄I₂N₄ Poids moléculaire: 668.39 Beilstein Registry Number: 3843838 Numéro CE: 247-081-0 PubChem CID: 104981

GRADE & PURITY ≥94%

Synonyms

3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide | PI

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Vue d’ensemble

Propidium iodide (PI) is a nuclear staining reagent that can stain DNA. Like EB, PI embeds into double stranded DNA and releases red fluorescence. Commonly used for detecting apoptosis or necrosis, and commonly used for flow cytometry analysis. Although PI cannot pass through live cell membranes, it can penetrate damaged cell membranes and stain nuclei. PI is often used in conjunction with Calcein, AM, Hoechst 33258, or Hoechst 33342 can be used together to stain both live and dead cells simultaneously. The excitation and emission wavelengths of PI-DNA complex are 535nm and 615nm, respectively.
Dyeing process:
(1) Prepare 10-50 μ M PI solution using PBS or appropriate buffer.
(2) Add 1/10 volume of PI solution to the cell culture medium (or use 1/10 concentration of PI buffer instead of medium).
(3) Cultivate cells at 37 ℃ for 10-20 minutes.
(4) Wash the cells twice with PBS or a suitable buffer.
(5) Observe cells under a fluorescence microscope using a filter with an excitation wavelength of 535nm and an emission wavelength of 615nm.

Specifications

Synonymes

3,8-Diamino-5-(3-(diethyl(methyl)ammonio)propyl)-6-phenylphenanthridin-5-ium iodide | PI

Spécifications et pureté

≥94%

Mécanismes biochimiques et physiologiques

Red-fluorescent DNA stain. Membrane impermeant to live cells. Propidium iodide staining differentiates between live and dead cells in cell populations.

Conditions de stockage de stockage

Protected from light,Store at -20°C,Argon charged

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Pureté

≥94%

Noms et identifiants

Pubchem Sid

508815492

Sourires canoniques

CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]

IUPAC Name

3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium;diiodide

InChIKey

XJMOSONTPMZWPB-UHFFFAOYSA-M

INCHI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

Isomères SMILES

CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]

WGK Allemagne

3

RTECS

SF7949600

PubChem CID

104981

Poids moléculaire

668.39

Beilstein

3843838

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Classe Quinolines and derivatives
      • Subclass Phenylquinolines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Classe: Quinolines and derivatives
• Subclass: Phenylquinolines
• Intermediate Tree Nodes: Not available
• Direct Parent: Phenylquinolines
• Alternative Parents: Phenanthridines and derivatives Phenylpyridines Isoquinolines and derivatives Benzene and substituted derivatives Tetraalkylammonium salts Heteroaromatic compounds Azacyclic compounds Primary amines Organopnictogen compounds Organic iodide salts Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Phenylquinoline - Benzoquinoline - Phenanthridine - 2-phenylpyridine - Isoquinoline - Monocyclic benzene moiety - Benzenoid - Pyridine - Tetraalkylammonium salt - Heteroaromatic compound - Quaternary ammonium salt - Azacycle - Organic salt - Organic iodide salt - Amine - Organonitrogen compound - Hydrocarbon derivative - Organopnictogen compound - Organic nitrogen compound - Primary amine - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as phenylquinolines. These are heterocyclic compounds containing a quinoline moiety substituted with a phenyl group.
• External Descriptors: organic iodide salt

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

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Mécanismes d'action

Propriétés chimiques et physiques

• Solubilité: Water (Slightly);DMSO (Slightly)
• Sensibilité: Hygroscopic,Light sensitive
• Point de fusion (°C): 220-225°C
• Poids moléculaire: 668.400 g/mol
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 7
• Exact Mass: 668.087 Da
• Monoisotopic Mass: 668.087 Da
• Topological Polar Surface Area: 55.900 Ų
• Heavy Atom Count: 33
• Formal Charge: 0
• Complexity: 555.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 3

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