Protein Sample Loading Buffer (Denaturing, Reducing, 5×) - BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5×

Cat. No.: S750308

GRADE & PURITY BioReagent, Suitable for electrophoresis, for PAGE, for Western blot, 5×

Synonyms

SDS-PAGE Sample Loading Buffer

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Electrophoresis, PAGE, SDS-PAGE, WB

Vue d’ensemble

  This product is designed for protein sample preparation in SDS-PAGE electrophoresis. Its novel formulation ensures a neutral pH after boiling, preventing protein degradation. It features fast thawing from –20°C and eliminates unpleasant odors by replacing traditional reducing agents (e.g., β-mercaptoethanol or DTT) with an advanced odorless alternative.
Key Features
1.Prevents Degradation: Neutral pH after boiling avoids protein degradation, ideal for phosphorylated proteins.
2.Rapid Settlement: Samples sink quickly to the bottom of wells without floating.
3.Fast Thawing: Rapid melting from –20°C without viscosity.
4.Odorless: Contains a novel reducing agent—no β-mercaptoethanol or DTT, no unpleasant smell.
Protocol
1.Add 1 volume of loading buffer to 4 volumes of protein sample (1:4 ratio) and mix thoroughly.
2.Heat the mixture in a boiling water bath for 5–10 minutes.
3.Centrifuge at high speed for 5 minutes. Use the supernatant for electrophoresis.
3.1Note: If no insoluble debris is observed after adding loading buffer, brief centrifugation is sufficient to collect the mixture.
3.2If condensation forms on the tube lid after heating, gently flick the tube to mix condensation with the sample before centrifugation to ensure uniform loading concentration.
Precautions
1.Gels loaded with this buffer may exhibit yellowing or streaks in silver staining due to the reducing agent.
2.Not suitable for non-denaturing gel electrophoresis as it contains denaturing agents.
3.Wear a lab coat and disposable gloves for safety.
4.For research use only.

Specifications

Synonymes

SDS-PAGE Sample Loading Buffer

Spécifications et pureté

BioReagent, suitable for electrophoresis, for PAGE, for western blot, 5×

Stabilité et stockage

Store at 2-8℃ short term (3 months). Store at -20℃ long term (12 months).

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

Adapté à l'électrophorèse, BioReagent, for PAGE, pour le Western blot