PTAD-Azide - ≥95%, used for PTAD-Azide is a selective crosslinking reagent that has one end for reacting with tyrosine and the other end for presenting an azide. After bioconjugation to tyrosine, the azide can be reacted with an alkyne through the Cu(I)-c

CAS: 1313211-52-7 Cat. No.: P467087 Formule: C₁₀H₁₀N₆O₃ Poids moléculaire: 262.22

GRADE & PURITY ≥95%

Synonyms

N₃₋Ph-Ur for e-Y-CLICK, 4-(4-(2-Azidoethoxy)phenyl)-1,2,4-triazolidine-3,5-dione

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

Vue d’ensemble

PTAD-Azide (4-(4-(2-Azidoethoxy)phenyl)-1,2,4-triazolidine-3,5-dione) is a 1,2,4-triazolidine-3,5-dione derivative. It can be prepared from ethyl hydrazinecarboxylate.

Note

PTAD-Azide must be first activated by stirring in a 1:0.98 molar ratio with 1,3-dibromo-5,5-dimethylhydantoin (product # 157902). Activation is evident upon solution color change from colorless to deep red and the activated reagent should be used immediately.
General Procedure for Protein Modification with PTAD.
Part 1: PTAD activation
Mix together 1:0.98 molar equivalents of unactivated PTAD to 1,3-dibromo-5,5-dimethylhydantoin (product # 157902) in organic solvent (preferred solvents are DMF or acetonitrile, avoid using DMSO)
Color change is observed from colorless/pale yellow to deep red (approximately 5 min of mixing).
After the solution turns red, store the now activated reagent on ice and use for protein modification within 30 min.
Part 2: Protein modification
Add protein solution in mixed phosphate/Tris buffer or Tris buffer (pH should be 6 - 9) to the eppendorf tube (or other vial) containing the activated PTAD reagent prepared above and mix gently at room temperature for up to 30 min. Preferably use 10-fold molar excess of reagent relative to protein. Use protein at a minimum concentration of 1 mg/ml (higher concentrations are preferred for enhanced labeling).
Remove excess unreacted PTAD by gel filtration.
PTAD-Azide may be used in the preparation of 4-(4-(2-azidoethoxy)phenyl)-3H-1,2,4-triazole-3,5(4H)-dione and aplaviroc-urazole.This urazole was recently demonstrated in the traceless, chemoselective labeling of peptides and proteins through electrochemical tyrosine-click (e-Y-CLICK) chemistry.  

Specifications

Synonymes

N₃₋Ph-Ur for e-Y-CLICK, 4-(4-(2-Azidoethoxy)phenyl)-1,2,4-triazolidine-3,5-dione

Spécifications et pureté

≥95%

Application

PTAD-Azide is a selective crosslinking reagent that has one end for reacting with tyrosine and the other end for presenting an azide. After bioconjugation to tyrosine, the azide can be reacted with an alkyne through the Cu(I)-catalyzed click chemistry rea

Conditions de stockage de stockage

Store at 2-8°C,Argon charged

Expédié en

Wet ice

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Pureté

≥95%

Noms et identifiants

• Sourires canoniques: O=C(NNC1=O)N1C2=CC=C(OCCN=[N+]=[N-])C=C2
• InChIKey: MHGMHPVYCVQIET-UHFFFAOYSA-N
• INCHI: 1S/C10H10N6O3/c11-15-12-5-6-19-8-3-1-7(2-4-8)16-9(17)13-14-10(16)18/h1-4H,5-6H2,(H,13,17)(H,14,18)
• Poids moléculaire: 262.22

Propriétés chimiques et physiques

• Sensibilité: Heat sensitive；Moisture sensitive
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable