Pyronine Y - Tache biologique , CAS No.92-32-0

CAS: 92-32-0 Cat. No.: P100851 Formule: C₁₇H₁₉N₂OCl Poids moléculaire: 302.81 Numéro CE: 202-147-8

GRADE & PURITY Biological Stain

Synonyms

tetramethyldiaminoxanthylium chloride | MFCD00011725 | NSC10454 | NSC-10454 | Pyronin Y, certified by the Biological Stain Commission, Dye content 50 % | W659G165T1 | AI3-52862 | Tetramethyl pyronin | Xanthylium, 3,6-bis(dimethylamino)-, chloride | AMMONI

Storage

Conserver à -20°C

Shipped In

Glacière + blocs de glace

Vue d’ensemble

La pyronine Y est un colorant qui s'intercale et colore l'ARN en rouge, et il a été démontré qu'elle s'accumule dans les mitochondries des cellules viables. Des études sur les Acinetobacter baumannii multirésistants (MDR) ont testé l'inhibition de la pompe à efflux en mesurant l'accumulation intracellulaire de pyronine Y et de bromure d'éthidium. La recherche montre qu'à de faibles concentrations de pyronine Y, l'effet cytostatique est l'arrêt des cellules en phase G1, alors qu'à des concentrations élevées, l'arrêt des cellules se fait en phase G2 et S. La pyronine Y a été utilisée en combinaison avec le bleu alcian pour évaluer et étudier les maladies pulmonaires, et en combinaison avec le vert de méthyle pour colorer les acides nucléiques dans les sections de paraffine. En outre, la pyronine Y a été utilisée pour mesurer l'ARN en cytométrie de flux.
Utilisée dans la coloration d'Unna-Pappenheim pour mettre en évidence l'ARN (rouge) par rapport à l'ADN (vert). Également utilisée pour la coloration des bactéries.

Specifications

Synonymes

tetramethyldiaminoxanthylium chloride | MFCD00011725 | NSC10454 | NSC-10454 | Pyronin Y, certified by the Biological Stain Commission, Dye content 50 % | W659G165T1 | AI3-52862 | Tetramethyl pyronin | Xanthylium, 3,6-bis(dimethylamino)-, chloride | AMMONI

Spécifications et pureté

Tache biologique

Mécanismes biochimiques et physiologiques

Colorant cationique qui s'intercale dans l'ARN et est visible sous la forme d'une bande rouge lors de l'électrophorèse. Il s'accumule dans les mitochondries des cellules viables. À faible concentration, l'effet cytostatique est l'arrêt de la cellule en ph

Conditions de stockage de stockage

Conserver à -20°C

Expédié en

Glacière + blocs de glace

Grade

Biological Stain

Note

Dans la mesure du possible, les solutions doivent être préparées et utilisées le même jour. Toutefois, si vous devez préparer des solutions de base à l'avance, nous vous recommandons de les conserver sous forme d'aliquotes dans des flacons hermétiques à -20°C. Ces aliquotes sont généralement utilisables pendant un mois. En général, ces solutions peuvent être utilisées pendant un mois. Avant utilisation, et avant d'ouvrir le flacon, nous vous recommandons de laisser votre produit s'équilibrer à température ambiante pendant au moins 1 heure. Besoin de plus de conseils sur la solubilité, l'utilisation et la manipulation ? Veuillez consulter notre page de questions fréquemment posées (FAQ) pour plus de détails.

Noms et identifiants

• Pubchem Sid: 504751244
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504751244
• Sourires canoniques: CN(C)C1=CC2=C(C=C1)C=C3C=CC(=[N+](C)C)C=C3O2.[Cl-]
• IUPAC Name: [6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium;chloride
• InChIKey: INCIMLINXXICKS-UHFFFAOYSA-M
• INCHI: 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
• Isomères SMILES: CN(C)C1=CC2=C(C=C1)C=C3C=CC(=[N+](C)C)C=C3O2.[Cl-]
• WGK Allemagne: 3
• Poids moléculaire: 302.81
• Reaxy-Rn: 3795789
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3795789&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Classe Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Classe: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Dialkylarylamines Benzenoids Heteroaromatic compounds Oxacyclic compounds Organopnictogen compounds Organooxygen compounds Organic chloride salts Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Benzenoid - Heteroaromatic compound - Tertiary amine - Oxacycle - Organic chloride salt - Hydrocarbon derivative - Organic salt - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Organooxygen compound - Organonitrogen compound - Amine - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: organic chloride salt - iminium salt

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

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Mécanismes d'action

Propriétés chimiques et physiques

• Solubilité: Soluble in water (30 mg/mL), methyl cellosolve (9 mg/mL), and ethanol (3 mg/mL).DMSO : 25 mg/mL (82.56 mM; Need ultrasonic)
• Sensibilité: light sensitive；Moisture sensitive
• Point de fusion (°C): 250-260°C
• Poids moléculaire: 302.800 g/mol
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 1
• Exact Mass: 302.119 Da
• Monoisotopic Mass: 302.119 Da
• Topological Polar Surface Area: 15.500 Ų
• Heavy Atom Count: 21
• Formal Charge: 0
• Complexity: 480.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3

Références

1. Mao Xu, Qi Tang, Yuchen Liu, Jiaqi Shi, Weiyu Zhang, Chan Guo, Qiuwen Liu, Weiwei Lei, Cheng Chen.  (2023)  Charged Boron Nitride Nanosheet Membranes for Improved Organic Solvent Nanofiltration.  ACS Applied Materials & Interfaces,      [PMID:36820819] [10.1021/acsami.2c20893]

2. Jing Liang, Qiang Shan, Tianqi Liu, Wenbin Liang, Xin Zhang, Wei Qi, Hongxin Tan, Shaobo Du, Lijuan Qian, Zhan Li, Ximeng Chen.  (2021)  One-step synthesis of annual ring-shaped planar nitrogen/sulfur co-doped nanoporous graphene for supercapacitance.  ELECTROCHIMICA ACTA,      [PMID:] [10.1016/j.electacta.2021.139137]

3. Junmei Fan, Enrui Wu, Jinlong Dong, Ruitao Zhu, Meiqin Li, Jiangning Gao, Hongfei Han, Liping Ding.  (2021)  A minimalist ratiometric fluorescent sensor based on non-covalent ternary platform for sensing H2S in aqueous solution and serum.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2021.126299]