Recombinant CD19 Antibody (PE) - Anticorps primaire, specific to Mouse CD19

Cat. No.: Ab213880

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

conseil

Reactive species

Souris

Isotype

Rat IgG2a

Clone Number

P99G09

Conjugation

PE

Vue d’ensemble

Recombinant CD19 Antibody, clone P99G09, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD19 Antibody (PE) - Anticorps primaire, specific to Mouse CD19

Synonymes

Antibody deficiency due to defect in CD19 antibody | Antibody deficiency due to defect in CD19, included antibody | AW495831 antibody | B lymphocyte antigen CD19 antibody | B lymphocyte surface antigen B4 antibody | B-lymphocyte antigen CD19 antibody | B-

Spécifications et pureté

ExactAb™, Validated, 0.5 mg/mL

Mécanismes biochimiques et physiologiques

Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Espèce hôte

conseil

Spécificité

Mouse CD19

Immunogène

Mouse CD19-expressing K562 human erythroleukemia cells

Contrôle positif

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

PE

Excitation(nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Émission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grade

ExactAb™, Validated

Type de chaîne lumineuse

kappa

Méthode de purification

Protein A purified

Concentration

0.5 mg/mL

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD19 Antibody (PE) (Ab213880)

Recombinant CD19 Antibody (PE) (Ab213880) - Flow Cytometry
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD19 Antibody (PE) (Ab213880) at 0.04μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: Recombinant CD19 Antibody (PE) (Ab213880)

Recombinant CD19 Antibody (PE) (Ab213880) - Flow Cytometry
Flow cytometry analysis of CD19 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD19 Antibody (PE) (Ab213880) at 0.04μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Cibles associées (humaines)

CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)