Recombinant CD46 Antibody - Anticorps primaire, specific to CD46, Rabbit IgG

Cat. No.: Ab094952

GRADE & PURITY Recombinant, ExactAb™, Validated, See COA

Application

IF/ICC, IHC, WB

Host species

Lapin

Reactive species

Humain

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human CD46 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/200

IF/ICC: 1/300
Protein Function：
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.

Specifications

Product Name

Recombinant CD46 Antibody - Anticorps primaire, specific to CD46, Rabbit IgG

Synonymes

Antigen defined by monoclonal antibody TRA 2 10 antibody | Antigen identified by monoclonal antibody TRA 2 10 antibody | CD46 antibody | CD46 antigen antibody | CD46 antigen complement regulatory protein antibody | CD46 molecule antibody | CD46 molecule c

Spécifications et pureté

Recombinant, ExactAb™, Validated, See COA

Espèce hôte

Lapin

Spécificité

CD46

Immunogène

A synthetic peptide derived from human CD46 (AA 30-87).

Contrôle positif

WB: K562, HeLa, Hep G2, MCF7 and MOLT-4 cell lysates. IHC: Human colon tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant CD46 Antibody (Ab094952)

Recombinant CD46 Antibody (Ab094952) - Western Blot
All lanes: Recombinant CD46 Antibody (Ab094952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 66 kDa
Exposure time: 11.4 s

Western Blot: Recombinant CD46 Antibody (Ab094952)

Recombinant CD46 Antibody (Ab094952) - Western Blot
All lanes: Recombinant CD46 Antibody (Ab094952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 66 kDa
Exposure time: 5.0 s

IHC: Recombinant CD46 Antibody (Ab094952)

Recombinant CD46 Antibody (Ab094952) - IHC
Immunohistochemical analysis of paraffin-embedded human colon, using Recombinant CD46 Antibody (Ab094952).

IF/ICC: Recombinant CD46 Antibody (Ab094952)

Recombinant CD46 Antibody (Ab094952) - IF/ICC
IF analysis of Recombinant CD46 Antibody (Ab094952) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant CD46 Antibody (Ab094952) at 1/300 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).

Application

Application

Dilution info

WB

1/500-1/2000

IHC

1/50-1/200

IF/ICC

1/300

Cibles associées (humaines)

CD46 Tbio Membrane cofactor protein (0 Activities)

Discover Target: CD46

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CD46 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.