Recombinant Dkk3 Antibody - Anticorps primaire, specific to DKK3, Rabbit IgG

Cat. No.: Ab099949

GRADE & PURITY Recombinant, Validated, ExactAb™, High Performance, 0.9 mg/mL

Application

WB

Host species

Lapin

Reactive species

conseil, Humain

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human Dkk3 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/1000-1/5000
Protein Function：
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Specifications

Product Name

Recombinant Dkk3 Antibody - Anticorps primaire, specific to DKK3, Rabbit IgG

Synonymes

dickkopf (Xenopus laevis) homolog 3 | dickkopf 3 | dickkopf homolog 3 (Xenopus laevis) | Dickkopf-3 | dickkopf-related protein 3 | Dkk3 | Dkk-3 | hDkk-3 | regulated in glioma | REIC | REICdkk-3 | RIG | RIG-like 5-6 | RIG-like 7-1 | Dickkopf 3 antibody | D

Spécifications et pureté

ExactAb™, Validated, Recombinant, High performance, 0.9 mg/mL

Espèce hôte

Lapin

Spécificité

DKK3

Immunogène

Recombinant protein of human Dkk3 (AA 251-350)

Contrôle positif

WB: Mouse brain, SK-N-SH and NIH/3T3 lysates.

Conjugation

Unconjugated

Grade

ExactAb™, High Performance, Recombinant, Validated

Isotype

Rabbit IgG

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

0.9 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant Dkk3 Antibody (Ab099949)

Recombinant Dkk3 Antibody (Ab099949) - Western Blot
All lanes: Recombinant Dkk3 Antibody (Ab099949) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 38 kDa
Observed band size: 59, 49 kDa.
The expression profile and molecular weight observed, which is due to glycosylation (PMID: 28674110, 28738084).
Exposure time: 200.0 s

Western Blot: Recombinant Dkk3 Antibody (Ab099949)

Recombinant Dkk3 Antibody (Ab099949) - Western Blot
All lanes: Recombinant Dkk3 Antibody (Ab099949) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 38 kDa
Observed band size: 52 kDa
Exposure time: 109.0 s

Application

• Application: Dilution info
• WB:

  1/1000 - 1/5000 ;

Cibles associées (humaines)

DKK3 Tbio Dickkopf-related protein 3 (0 Activities)

Discover Target: DKK3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Dkk3 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.