Recombinant Human HPRG Protein

Cat. No.: rp147069

QUALITÉ & PURETÉ Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His Tag, ≥90%(SDS-PAGE)

Accession #

P04196

Protein Tag

C-10His

Expression system

HEK293

Endotoxin Concentration

<0.1 EU/μg

Bioactivity

Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. When 1 x 10^5 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.4-2 μg/mL.

Vue d’ensemble

Function
Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.

Spécifications

Product Name

Recombinant Human HPRG Protein

Synonymes

Histidine-proline-rich glycoprotein | HPRG | DKFZp779H1622 | Histidine-proline-rich glycoprotein | histidine-rich glycoprotein | HPRG | HRGP | HRG | Thrombophilia due to elevated HRG | THPH11

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag

Spécifications et pureté

Animal Free,Carrier Free,Bioactive,ActiBioPure™,High Performance,His-Tag,≥90%(SDS-PAGE)

Bioactivité

Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. When 1 x 10^5 cells/well are added to Recombinant Human HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.4-2 μg/mL.

Concentration d'endotoxine

<0.1 EU/μg

Système d'expression

HEK293

Espèces

Human

Acides aminés

19-525 aa

Séquence

VSPTDCSAVEPEAEKALDLINKRRRDGYLFQLLRIADAHLDRVENTTVYYLVLDVQESDCSVLSRKYWNDCEPPDSRRPSEIVIGQCKVIATRHSHESQDLRVIDFNCTTSSVSSALANTKDSPVLIDFFEDTERYRKQANKALEKYKEENDDFASFRVDRIERVARVRGGEGTGYFVDFSVRNCPRHHFPRHPNVFGFCRADLFYDVEALDLESPKNLVINCEVFDPQEHENINGVPPHLGHPFHWGGHERSSTTKPPFKPHGSRDHHHPHKPHEHGPPPPPDERDHSHGPPLPQGPPPLLPMSCSSCQHATFGTNGAQRHSHNNNSSDLHPHKHHSHEQHPHGHHPHAHHPHEHDTHRQHPHGHHPHGHHPHGHHPHGHHPHGHHPHCHDFQDYGPCDPPPHNQGHCCHGHGPPPGHLRRRGPGKGPRPFHCRQIGSVYRLPPLRKGEVLPLPEANFPSFPLPHHKHPLKPDNQPFPQSVSESCPGKFKSGFPQVSMFFTHTFPKHHHHHHHHHH

Journée des protéines

C-10His

Accession #

P04196

Poids moléculaire prévu

59 kDa

SDS-PAGE

65 -75 kDa, under reducing conditions; 55 - 65 kDa, under non-reducing conditions.

Type de molécule

Protein

Product Line

Produits biologiques

Stockage et expédition

Forme

Lyophilized

Reconstitution

Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Recombinant Human HPRG Protein (rp147069)

Recombinant Human HPRG Protein (rp147069) - SDS-PAGE
2 μg/lane of Recombinant Human HPRG Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 65 -75 kDa under reducing conditions and 55 - 65 kDa under non-reducing conditions.