Recombinant TORC2 Antibody - Anticorps primaire, specific to CRTC2, Rabbit IgG

Cat. No.: Ab132016

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.7 mg/mL

Application

IHC, WB

Host species

Lapin

Reactive species

conseil, Humain, Souris

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human TORC2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
Protein Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

Specifications

Product Name

Recombinant TORC2 Antibody - Anticorps primaire, specific to CRTC2, Rabbit IgG

Synonymes

CREB regulated transcription coactivator 2 | CREB-regulated transcription coactivator 2 | CRTC2 | TORC2 | TORC-2 | TORC2transducer of regulated cAMP response element-binding protein (CREB) 2 | Transducer of CREB protein 2 | Transducer of regulated cAMP re

Spécifications et pureté

ExactAb™, Validated, Recombinant, 0.7 mg/mL

Espèce hôte

Lapin

Spécificité

CRTC2

Immunogène

A synthetic peptide derived from human TORC2 (AA 680-693).

Contrôle positif

WB: Jurkat, Raji, HEK293, NIH/3T3 and C6 cell lysates. IHC：Human breast cancer tissue.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Immunogen affinity purified

Concentration

0.7 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant TORC2 Antibody (Ab132016)

Recombinant TORC2 Antibody (Ab132016) - Western Blot
All lanes: Recombinant TORC2 Antibody (Ab132016) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 73 kDa
Observed band size: 81 kDa
Exposure time: 13.3 s

IHC: Recombinant TORC2 Antibody (Ab132016)

Recombinant TORC2 Antibody (Ab132016) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant TORC2 Antibody (Ab132016). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50 - 1/100;

Cibles associées (humaines)

CRTC2 Tbio CREB-regulated transcription coactivator 2 (0 Activities)

Discover Target: CRTC2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant TORC2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.