Recombinant U2AF65 Antibody - Anticorps primaire, specific to U2AF2, Rabbit IgG

Cat. No.: Ab133178

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

WB

Host species

Lapin

Reactive species

conseil, Humain, Souris

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human U2AF65 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
Protein Function
Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.
Post-translational
Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events.

Specifications

Product Name

Recombinant U2AF65 Antibody - Anticorps primaire, specific to U2AF2, Rabbit IgG

Synonymes

hU2AF(65) antibody | hU2AF65 antibody | Splicing factor U2AF 65 kDa subunit antibody | U2 (RNU2) small nuclear RNA auxiliary factor 2 antibody | U2 auxiliary factor 65 kDa subunit antibody | U2 small nuclear ribonucleoprotein auxiliary factor (65kD) antib

Spécifications et pureté

Recombinant, ExactAb™, Validated, See COA

Espèce hôte

Lapin

Spécificité

U2AF2

Immunogène

A synthesized peptide derived from human U2AF65 (AA 415-475)

Contrôle positif

WB: HeLa, Jurkat, Hep G2, HEK293, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant U2AF65 Antibody (Ab133178)

Recombinant U2AF65 Antibody (Ab133178) - Western Blot
All lanes: Recombinant U2AF65 Antibody (Ab133178) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 40, 59 kDa
Exposure time: 19.7 s

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

Cibles associées (humaines)

U2AF2 Tbio Splicing factor U2AF 65 kDa subunit (0 Activities)

Discover Target: U2AF2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant U2AF65 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.