Soybean-Casein Digest Broth (USP) - BioReagent

Cat. No.: S1528366

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

For sterility testing of pharmaceuticals. Preparation method: Weigh 30.0 g of this product in 1 L of distilled or deionized water, heat to boiling until completely dissolved, autoclave at 121°C for 15 minutes, and set aside for use.

Specifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Product Line

Produits biologiques

Application Protocols

• Item-specific, validated application parameters: Not specified for this item; refer to CoA/Spec Sheet and SDS.
• General/literature protocols
  • Preparation of 1 L broth: Add 30 g dehydrated medium to ~800 mL purified water; heat with stirring to dissolve; bring to 1 L; distribute into suitable vessels; autoclave 121 °C for 15 min; cool to 45–50 °C; tighten closures. Store as per lab SOP.
  • Sterility testing (overview): Aseptically inoculate test articles or rinse fluids into the broth according to your method; incubate at specified temperature/time; examine for turbidity. Perform positive/negative controls and growth promotion per SOP. Validate for product-specific neutralization if inhibitors are present.
  • Environmental monitoring: Expose contact plates/swabs into the broth for enrichment; incubate with appropriate aeration; subculture to differential/selective media as required.
  • Bioburden testing: Pre-enrich samples in this broth before enumeration or identification; include neutralizers if disinfectant residues are expected.
• Notes
  • Always confirm incubation temperatures, times, and interpretation criteria with your internal SOPs and pharmacopeial chapters. The above are general guidelines, not item-specific specifications.

Biological Roles

• Nature of product
  • A non-selective, nutrient-rich medium that provides carbon, nitrogen, minerals, and buffering capacity to support microbial metabolism and proliferation.
• Functional components (literature; typical composition of Soybean-Casein Digest Broth)
  • Pancreatic digest of casein (~17 g/L): Peptides and free amino acids supply nitrogen, sulfur-containing amino acids, and growth factors.
  • Papaic digest of soybean meal (~3 g/L): Additional peptides, vitamins, and carbohydrates; contributes to a broader nutritional profile.
  • Sodium chloride (~5 g/L): Maintains osmotic balance approximating physiological conditions.
  • Dextrose (~2.5 g/L): Readily metabolizable carbon/energy source for many bacteria.
  • Dipotassium phosphate (~2.5 g/L): Provides buffering near neutral pH and essential potassium/phosphate.
  • Note: Values above are typical literature values and not specifications for this item; refer to CoA/Spec Sheet for lot-specific details.
• Biological performance (general/literature)
  • Supports robust growth of many aerobic and facultative anaerobic bacteria and some yeasts/molds, making it suitable for general cultivation and enrichment.
  • Phosphate buffering helps stabilize pH against acidification from carbohydrate metabolism during early growth phases.
• Use limitations
  • Not selective or differential; for targeted recovery of fastidious or stressed organisms, supplementation (e.g., neutralizers, blood) or alternative media may be required.

Buffer Applications

This product is a nutrient medium and not a dedicated buffering agent.

• General note (literature)
  • Contains dipotassium phosphate, providing modest buffering capacity around neutral pH in the prepared broth (typical pH ~7.3 ± 0.2 post-sterilization). However, it is not formulated to function as a stand-alone buffer system.
• Practical guidance
  • For experiments requiring precise pH control independent of nutrient composition, use defined buffers (e.g., phosphate, HEPES, MOPS) prepared separately. The broth’s nutrient load can confound buffer-only applications.

Green Alternatives

• Context

  • This product is already water-based and free of organic solvent use during routine preparation. Sustainability considerations focus on sourcing, waste minimization, and energy used in sterilization.

• Greener choices and trade-offs (general)

  • Plant-based peptones: Soybean digest is plant-derived; some labs adopt fully animal-free formulations to reduce animal-origin inputs. Trade-off: growth spectrum may shift; validate equivalency.
  • Concentrate preparation: Prepare only the volume required to minimize disposal of unused sterile broth.
  • Energy efficiency: Optimize autoclave loads and cycles to reduce energy/water consumption; use validated cycle times compatible with your load.
  • Reusable glassware vs. single-use plastics: Reuse reduces plastic waste but increases washing/sterilization energy; balance based on facility capabilities and contamination risk.

• Comparison snapshot (literature/general)

  | Option | Resource impact | Performance considerations | |---|---|---| | Standard TSB (soy-casein) | Baseline | Broad growth; well-characterized | | Animal-origin–free (AOF) peptones | Reduced animal inputs | May require GPT requalification | | Ready-to-use sterile broth | Higher packaging/waste | Saves prep time; consistent but less flexible | | In-house autoclaving | Energy/water use | Maximum control; scalable |

• Waste management

  • Decontaminate spent media by autoclaving or validated chemical disinfection before drain disposal per local regulations.

Pharmaceutical Uses

• Item-specific restriction
  • Research Use Only (from Product Data). Not for use in diagnostic procedures or as a therapeutic agent.
• General/literature context in pharma/biomanufacturing labs
  • Commonly employed as a non-selective medium in environmental monitoring, bioburden assessments, and growth promotion tests when validated by the user.
  • Frequently used in sterility testing workflows as a fluid medium per pharmacopeial methods; verify suitability, growth promotion, and pH on the lot-specific CoA/Spec Sheet before regulated use.
  • Utility as a pre-enrichment broth prior to selective media for microbial limits testing of raw materials and intermediates.
• Formulation/excipient status
  • Not used as a pharmaceutical excipient. Its role is confined to quality control microbiology and research culture work.

Physical Properties

• Item-specific facts (from Product Data)
  • Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Storage conditions: Room temperature (provided)
  • Shipping: Normal (provided)
• General/Literature characteristics (for Soybean-Casein Digest Broth, dehydrated)
  • Typical form: Dehydrated powder that readily dissolves in water (literature)
  • Typical working concentration: 30 g per liter of purified water to prepare broth (literature)
  • Typical pH of prepared medium: ~7.3 ± 0.2 at 25 °C after sterilization (literature)
  • Solubility: Freely soluble in water; insoluble in most organic solvents (literature)
  • Color: Commonly light tan to beige (varies by lot and supplier; literature)
• Not applicable to complex media (mixtures)
  • Boiling point, melting point, density, refractive index, logP, pKa: Not applicable/not defined for heterogeneous mixtures; not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Item-specific information (from Product Data)
  • Grade: BioReagent
  • Product name includes “(USP)”. This denotes the formulation aligns with the United States Pharmacopeia description for Soybean-Casein Digest Medium; actual compliance and test results should be verified on the product’s CoA/Spec Sheet.
• What BioReagent grade typically implies (general)
  • Suitable for biological and microbiological research applications, with controlled bioburden and performance characteristics appropriate for lab use.
  • Not necessarily GMP- or clinical-grade; not intended for human or animal therapeutic use.
• USP contextual notes (general/literature)
  • USP describes performance tests (growth promotion, sterility, pH) for sterility test media. Vendors commonly qualify lots for robust growth of specified organisms.
  • For regulated workflows (e.g., sterility testing), verify: growth promotion test results, pH after sterilization, clarity, and sterility on the lot-specific CoA.
• Stabilizers/antimicrobials
  • No antimicrobial inhibitors are present in standard Soybean-Casein Digest Broth formulations (literature). If any additives are included, they must be confirmed on the label/CoA. For this item: Not specified; refer to CoA/Spec Sheet.

Reaction and Applications

As a microbiological medium, this product is used for cultivating a broad range of microorganisms; it is not a reagent for chemical reactions.

• Typical laboratory applications (general/literature)
  • Growth and maintenance of aerobic and facultative anaerobic bacteria from clinical, environmental, food, and industrial sources.
  • Sterility testing workflows where a non-selective, nutrient-rich broth is required (verify suitability with your method SOP and CoA).
  • Pre-enrichment step prior to selective plating or biochemical identification.
  • Environmental monitoring and bioburden testing in pharmaceutical/manufacturing settings (procedural compliance to be established by the user).
• Practical tips (general)
  • Aseptic technique is essential; pre-sterilize vessels and closures. Cool sterilized broth to ~45–50 °C before supplementing (e.g., neutralizers) to preserve activity.
  • For anaerobes or microaerophiles, consider reducing agents (e.g., sodium thioglycollate) or appropriate atmospheric controls; TSB alone is primarily suited to aerobic/facultative organisms.
  • Perform growth promotion testing (GPT) with specified challenge strains on new lots to confirm expected performance under your conditions.
  • If clarity/turbidity readings are critical, ensure glassware cleanliness and avoid caramelization during sterilization.
• Not typically applicable
  • Named organic reactions and catalytic chemistry are not relevant to this product type.

Reaction Conditions

Here, “reaction” refers to microbiological culture preparation rather than chemical transformations.

• General preparation (literature guidance; not item-specific specifications)
  • Working concentration: 30 g dehydrated medium per 1 L purified water; mix while warming until fully dissolved.
  • pH: Adjust if necessary to ~7.3 ± 0.2 at 25 °C (after sterilization, measured when cooled).
  • Sterilization: Autoclave at 121 °C (15 psi) for 15 minutes. Do not overheat to prevent sugar caramelization and Maillard browning.
  • Cooling and dispensing: Cool to ~45–50 °C before aseptic dispensing or addition of heat-labile supplements (e.g., neutralizers).
• Culture conditions (general)
  • Incubation temperature: Select per organism (e.g., 20–37 °C for many mesophiles). Aeration/agitation improves growth of aerobes/facultative anaerobes.
  • Expected performance: Rapid turbidity development with many nonfastidious bacteria within 6–24 h, depending on inoculum and temperature.
• Quality checks (general)
  • Clarity: Prepared broth should be clear to slightly opalescent; any pre-inoculation turbidity or precipitate may indicate improper dissolution or contamination.
  • Growth promotion testing: Verify recovery of compendial strains as required by your SOP.
• Note
  • Values above are literature guidance and not specifications for this item; for this SKU, refer to CoA/Spec Sheet for exact preparation and QC parameters.

Safety and Handling

• Item-specific hazard information
  • Signal word, H-statements, GHS classification, pictograms: Not specified for this item; refer to SDS.
• General safety considerations for dehydrated culture media (literature/practice)
  • Avoid dust generation and inhalation; dust may cause mechanical/respiratory irritation.
  • Contains soy- and milk-derived components (soybean digest and casein digest); individuals with sensitivities should avoid exposure.
  • Use standard laboratory PPE: lab coat, safety glasses, nitrile gloves; handle in a clean environment to avoid pre-use contamination.
  • Incompatibilities: Strong oxidizers and strong acids/bases may degrade medium components; store away from moisture and volatile chemicals.
  • First-aid (overview; consult SDS): If inhaled—move to fresh air; skin/eye contact—rinse with water; ingestion—rinse mouth. Seek medical advice if symptoms persist.
  • Microbiological risk: After inoculation, medium may contain viable microorganisms; treat all used media and contaminated disposables as biohazardous waste. Decontaminate by autoclaving or appropriate disinfectants before disposal.
• Fire safety
  • Combustibility: Organic content is combustible; use standard extinguishing media (water spray, CO2, dry chemical). No unusual combustion products beyond those typical for nitrogenous organics are expected (consult SDS).
• Always consult the product’s SDS and institutional biosafety policies for authoritative guidance.

Solvent Selection

This product is a dehydrated medium intended to be dissolved in purified water; organic solvent selection is not applicable.

• Item-specific note
  • Recommended solvent: Not specified for this item; refer to CoA/Spec Sheet. In general, high-purity water is used.
• General/literature guidance for reconstitution
  • Water quality: Use distilled, deionized, or purified water meeting microbiological media prep standards (e.g., ≤10 CFU/mL, low TOC) to avoid inhibitory contaminants.
  • Dissolution: Gradual addition to water with stirring; gentle heating may aid dissolution. Avoid localized overheating that can caramelize sugars.
  • Sterilization: Autoclave at 121 °C for 15 min or sterile-filter where applicable (literature). Allow to cool to ~45–50 °C before aseptic dispensing.
• Comparison (general)
  • Purified water vs. tap water: Tap water may contain chlorine/chloramine and metals that inhibit growth; use purified water for reproducibility.
  • Broth vs. agar: For solid media, the corresponding Soybean-Casein Digest Agar contains agar; this broth is for liquid culture and tests requiring turbidity measurement.

Storage and Reconstitution

• Item-specific storage and shipping
  • Storage conditions: Room temperature (provided)
  • Shipped: Normal (provided)
• Item-specific appearance and shelf life
  • Appearance and shelf life: Not specified for this item; refer to CoA/Spec Sheet and product label for expiration dating.
• General/literature guidance for reconstitution and storage
  • Protect the dehydrated medium from moisture, excessive heat, and direct sunlight. Keep container tightly closed; use a desiccant where appropriate.
  • Reconstitution: Dissolve the recommended mass of dehydrated medium in purified water (typical literature value: 30 g/L), mix until dissolved, adjust pH if needed, and sterilize by autoclaving at 121 °C for 15 minutes. Allow to cool before use.
  • Prepared medium storage: Store sterilized broth at 2–8 °C when not in immediate use; warm to room temperature before inoculation. Avoid repeated temperature cycling.
  • Freeze–thaw: Not applicable; do not freeze prepared medium as precipitation may occur and performance may be affected.
  • Aseptic handling: Use sterile technique to prevent pre-use contamination; discard if turbidity develops prior to inoculation.
• Always consult the lot-specific CoA/Spec Sheet and SDS for authoritative instructions for this SKU.

Structure and Identity

This product is a complex, dehydrated microbiological growth medium (commonly known as Tryptic Soy Broth, TSB) and does not have a single defined molecular structure.

• Item-specific facts (from Product Data)
  • Product name: Soybean-Casein Digest Broth (USP)
  • SKU: S1528366
  • Grade: BioReagent
  • CAS, CID, InChIKey, SMILES: Not applicable to complex media; not specified for this item.
• General description (literature)
  • Composition class: Complex mixture of peptides, amino acids, carbohydrates, salts, and phosphate buffer derived from pancreatic digest of casein and papaic digest of soybean meal, with sodium chloride, dextrose, and dipotassium phosphate.
  • Functional groups present: Amide/peptide bonds (in peptones), carbohydrate hydroxyls (dextrose), inorganic salts and phosphate.
  • 2D structural features: Not applicable; the product is a heterogeneous blend rather than a single molecule.
• Molecular formula and molecular weight
  • Not applicable to mixtures; not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

This product is not a synthetic chemistry reagent and is not typically used in organic or inorganic synthesis.

• Practical note for chemists working across disciplines
  • If sterile conditions or microbial degradation are concerns in a chemical workflow, avoid introducing complex media; residual proteins/peptides and sugars can complicate analyses and foul chromatography.
  • For biocatalysis or whole-cell transformations, a defined medium may be preferable to control nutrient effects on enzyme expression and byproduct profiles. Tryptic Soy Broth is non-defined and can add variability.

Target Specificity

Not applicable. This product is a general-purpose microbiological medium and is not a targeted biomolecule (e.g., antibody, enzyme, probe). It does not possess antigen/epitope specificity.