Standard I Nutrient Broth - BioReagent

Cat. No.: S1528289

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

For bacterial cultivation, nutrient-rich

Specifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Product Line

biological_products

Application Protocols

• General broth preparation (follow label instructions for this item; values below are literature examples, not item-specific)

  1. Weigh the manufacturer-recommended mass of dehydrated powder and add to purified water to the desired final volume (literature: often 8–13 g per liter).
  2. Stir until completely dissolved; adjust pH only if directed by the label.
  3. Dispense into culture vessels and autoclave at 121 °C for 15 minutes. Cool to 45–50 °C if supplements will be added aseptically.

• Routine cultivation

  1. Inoculate sterile broth with the test organism to a defined starting density (e.g., 0.01–0.05 OD600 or a specified McFarland standard), using aseptic technique.
  2. Incubate at the organism-appropriate temperature (commonly 30–37 °C) with agitation (150–250 rpm) for aerophiles; static for facultative/anaerobes as needed.
  3. Monitor turbidity (OD600) or plate serial dilutions for CFU enumeration at defined intervals.

• Growth promotion testing (example framework; tailor to SOP)

  • Inoculate with low-level ATCC control strains (e.g., E. coli, S. aureus, P. aeruginosa) to achieve 10–100 CFU per vessel. Incubate under method-specified conditions and verify recovery and characteristic turbidity within acceptance windows.

• Sterility control

  • Incubate an uninoculated control in parallel to confirm medium sterility post-sterilization.

• Waste handling

  • Autoclave spent cultures/liquids prior to disposal per institutional biosafety procedures.

Biological Roles

• Function of the medium (general, literature)

  • Provides assimilable nitrogen (short peptides, amino acids) and carbon sources (amino acids, small carbohydrates), vitamins, and minerals to support heterotrophic bacterial growth.
  • Sodium chloride (when present) maintains osmotic balance, aiding viability and morphology maintenance.

• Microbial physiology context

  • Peptides are transported via oligopeptide permeases and hydrolyzed by intracellular peptidases, fueling central carbon metabolism (TCA, glycolysis) and biosynthesis.
  • Complex media dampen selective pressures and can mask auxotrophies; they are suited for general cultivation and recovery of stressed cells.

• Experimental implications

  • Growth rates, lag times, and expression profiles can differ markedly from defined media. For physiological studies, document medium composition/lot and consider running parallel defined-medium controls.
  • Buffered variants (if used) stabilize pH as ammonium and organic acid byproducts accumulate; unbuffered nutrient broths may acidify or alkalinize depending on substrate utilization.

• Scope

  • Designed for research use only. Not intended for therapeutic, diagnostic, or clinical use without appropriate validation and regulatory compliance.

Buffer Applications

This product is not a dedicated buffer system. It may exhibit limited intrinsic buffering capacity due to amino acid and phosphate (if present) equilibria, but it is formulated primarily for microbial nutrition rather than precise pH control.

• Practical notes (general)
  • If stringent pH control is required (e.g., enzyme assays), supplement with a defined buffer (e.g., phosphate, MOPS) compatible with the organism and experiment.
  • Check and record initial pH of the reconstituted broth at the working temperature; biological activity can shift pH during incubation.

Green Alternatives

• Environmental aspects (general)

  • Dehydrated broths are water-based and nonflammable, minimizing solvent emissions and VOCs during preparation. Primary impacts arise from energy used in sterilization and disposal of biohazardous waste.

• Greener practice recommendations

  • Optimize batch sizes to reduce autoclave cycles; consolidate sterilizations and use validated liquid cycles to prevent overprocessing.
  • Consider plant-derived peptones (animal-free nutrient broths) to reduce reliance on animal by-products where appropriate.
  • Implement closed-bottle culture systems and reusable glassware with validated cleaning to reduce single-use plastics.
  • Treat spent media on-site (autoclave) to inactivate biological content before disposal, reducing the need for chemical disinfectants.

• Comparison (literature/general)

  | Option | Advantages | Trade-offs | |---|---|---| | Standard nutrient broth | Broad organism compatibility; simple formulation; cost-effective | Animal-derived components in many formulations; lot variability | | Plant/soy peptone broth | Lower animal footprint; often clearer filtrates | May alter growth kinetics for certain strains—requires requalification | | Defined minimal media | High reproducibility; reduced organic load in waste | Narrower organism range; requires more supplements; not suitable for routine recovery |

• Takeaway

  • Selection should balance organism requirements, sustainability goals, and method compliance. Validate greener substitutions with growth promotion tests.

Pharmaceutical Uses

• Context (general)

  • Nutrient broths are commonly used in pharmaceutical and biomanufacturing laboratories for in-process controls, environmental monitoring, growth promotion testing, and as nonselective recovery media during method suitability tests (per compendial guidance), when permitted by internal procedures.

• Important caveats

  • This product is labeled BioReagent for research use only. It is not specified as GMP-grade or compendial-grade; therefore, suitability for regulated applications must be determined by the end user with appropriate qualification and documentation.

• Practical considerations

  • For sterility or bioburden testing, compendial media (e.g., Soybean-Casein Digest, Fluid Thioglycollate) are more commonly mandated; nutrient broth may serve for growth promotion of nonfastidious strains or as a general-purpose diluent.
  • Lot-to-lot verification should include growth promotion with specified ATCC strains, sterility checks, pH confirmation, and clarity/turbidity baselines post-sterilization.

Physical Properties

• Item-specific values

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • pH (reconstituted): Not specified for this item; refer to CoA/Spec Sheet.
  • Solubility: Not specified for this item; refer to CoA/Spec Sheet.
  • Moisture content/ash: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/general properties for nutrient broths (not item-specific)

  • Form: Free-flowing, tan-to-beige dehydrated powder; readily water-soluble.
  • Typical working concentration: 8–13 g/L in purified water (varies by standard and brand); adjust per label.
  • Typical pH (25 °C, after autoclaving): ~7.2–7.4 (literature ranges 6.8–7.4 depending on formulation).
  • Osmolality: commonly in the 200–400 mOsm/kg range at use-strength, depending on NaCl and extract content.
  • Density (dry powder): bulk density often 0.4–0.7 g/mL; true density not generally reported for complex media.
  • Refractive index/boiling point/melting point: Not applicable in the conventional sense—product is a multicomponent solid; working solutions are aqueous and display water-like phase behavior.

• Notes for practice

  • Dissolution is endothermic-to-neutral; stir vigorously to avoid clumping. Allow bubbles to dissipate before dispensing.
  • Final pH depends on water quality and exact lot composition; verify pH and adjust only if your test method/spec allows (most standardized microbiology methods require no pH adjustment beyond label instructions).

Quality and Grades

• Item-specific grade

  • Grade/Purity: BioReagent (as listed). This denotes suitability for biological research applications where undefined complex media components are acceptable and lot-to-lot performance is controlled.

• What BioReagent implies (general)

  • Sourced and manufactured for laboratory research, including microbiology and cell-free assays. Emphasis on performance metrics (e.g., growth promotion testing of representative strains), bioburden control in the dehydrated powder, and stability.
  • Not produced to clinical diagnostic, GMP, or pharmacopeial monographs unless explicitly stated. For regulated work, qualify the lot with in-house QC.

• Lot qualification (recommended practice)

  • Review CoA for: growth promotion test organisms and acceptance criteria, pH at working strength, visual clarity, and sterility of uninoculated control following autoclave/filtration.
  • For method validation, perform side-by-side comparison versus your historical medium and document colony counts/turbidity kinetics.

• Stabilizers and additives

  • No stabilizers are specified for this item; refer to CoA/Spec Sheet.
  • Many nutrient broths rely on intrinsic stability of dehydrated peptones/extracts; antioxidants/antifoams are typically absent unless declared.

Reaction and Applications

This product is not used for synthetic chemical reactions; its “applications” are microbiological.

• Typical research uses (literature/general for nutrient broths)

  • Cultivation and maintenance of nonfastidious bacteria (e.g., Enterobacteriaceae, Pseudomonas spp., Bacillus spp.) for routine propagation and QC.
  • Inoculum preparation prior to downstream assays (antimicrobial susceptibility testing, bacteriophage amplification, transformation pre-cultures).
  • Turbidity-based growth studies (OD600 kinetics), growth promotion testing, and media qualification controls.
  • Recovery medium following environmental sampling or surface swabs before selective plating.
  • Diluent for serial dilution of cultures when isotonicity is required (when NaCl is present in the formulation) and minimal nutrient carryover is acceptable.

• Practical tips

  • Use freshly prepared medium or verify sterility of stored aliquots. Avoid caramelization or Maillard browning from prolonged heating, which can reduce growth.
  • For fastidious organisms, enrich with supplements (e.g., yeast extract, glucose) as dictated by the method—filter-sterilize supplements post-autoclave to preserve heat-labile nutrients.
  • Standardize inoculum density (e.g., McFarland standards) to ensure reproducibility of growth curves and antimicrobial assays.
  • Document lot numbers in all comparative studies; complex media can show subtle performance shifts across lots.

Reaction Conditions

For this product, “conditions” refer to media preparation and sterilization rather than chemical reaction conditions.

• General preparation (literature; follow the product label for exact instructions)

  • Dissolve the indicated mass of dehydrated powder in purified water to the specified final volume (typical working strengths for nutrient broths range ~8–13 g/L, but follow the label for this item). Mix until fully dissolved.
  • Distribute into suitable vessels (e.g., flasks, tubes, bottles) with appropriate headspace to prevent boil-over.
  • Sterilize by autoclaving at 121 °C for 15 minutes (liquid cycle). Allow to cool to handling temperature before inoculation. Do not overheat to avoid Maillard browning.
  • If adding heat-labile supplements (e.g., carbohydrates, antibiotics), sterile-filter those separately and aseptically add to cooled sterile broth.

• Quality checks

  • Confirm pH at room temperature (expected neutral range per literature, exact value per CoA). Inspect for clarity and absence of precipitates; discard if turbidity is present before inoculation.
  • Perform sterility controls (uninoculated incubations) alongside experimental cultures.

• Storage of prepared medium

  • Refrigerate sterile broth (2–8 °C) and use within a few weeks; rewarm to room temperature before use. Always validate shelf-life for critical work.

Safety and Handling

• GHS and hazard information (item-specific)

  • Signal word: Not specified for this item; refer to SDS.
  • Hazard statements (H-): Not specified for this item; refer to SDS.
  • GHS classification/pictograms: Not specified for this item; refer to SDS.

• General safety guidance (literature/general)

  • Nutrient broths are typically nonflammable, nonvolatile powders but can cause dust irritation. Avoid inhalation and contact with eyes/skin. Use standard lab PPE: lab coat, gloves, safety glasses.
  • Handle as potentially biohazardous AFTER inoculation. All spent media and cultures must be autoclaved (e.g., 121 °C, ≥15 min) or treated per institutional biosafety protocols before disposal.
  • Incompatibilities: Strong oxidizers may degrade organic components; moisture can cause caking and microbial contamination—keep container tightly closed.
  • First aid (overview; defer to SDS): Inhalation—move to fresh air; Eye/Skin—rinse with water; Ingestion—rinse mouth. Seek medical attention if irritation persists.
  • Thermal processing: Use caution when autoclaving; depressurize slowly. Do not overfill containers; loosen caps to prevent bursting.

• Environmental considerations

  • Avoid discharge of live cultures to drains. Treat liquid waste with approved disinfectants or autoclaving prior to disposal per local regulations.

Solvent Selection

This product is a dehydrated culture medium intended to be dissolved in water; organic solvent selection is not applicable.

• Practical guidance (general)

  • Solvent: Use purified water (e.g., ASTM Type 1/2, deionized, or equivalent). High ionic content or residual disinfectants can inhibit growth.
  • Miscibility: Completely miscible in water at working concentrations.
  • Filtration: If sterile filtration is chosen (instead of autoclaving heat-labile supplements), use 0.22 µm PES or PVDF membranes; prefilter if particulates persist.

• Comparison with alternatives (literature/general)

  • Nutrient Broth vs LB Broth: Nutrient Broth is less rich and more suitable for baseline growth and physiological studies without excessive catabolite repression; LB is richer and may accelerate growth but can alter expression profiles.
  • Animal-free variants: Plant/soy peptone-based broths reduce animal-derived components; consider if required by policy or application.

Storage and Reconstitution

• Item-specific storage/shipping

  • Storage Conditions: Room temperature (per product data). Protect from moisture and microbial contamination; keep container tightly closed.
  • Shipped In: Normal (ambient) conditions.

• Reconstitution guidance

  • Working concentration: Not specified for this item; refer to CoA/Spec Sheet/label for the exact grams per liter and target pH.
  • Water quality: Use purified water. Avoid residual sanitizers (chlorine, quats) that may inhibit growth.
  • Dissolution: Add powder slowly to water with vigorous stirring to prevent clumping; rinse weighing vessel to ensure quantitative transfer.

• Sterilization and supplements

  • Autoclave at 121 °C for 15 minutes unless the label indicates alternative conditions. Cool before adding heat-labile supplements via sterile filtration (0.22 µm).

• Prepared-media storage (general)

  • Store sterile prepared broth at 2–8 °C, protected from light. Typical in-use shelf-life is days to a few weeks depending on container and aseptic handling; validate per your SOP.
  • Avoid freeze–thaw of prepared media; precipitation or performance drift can occur.

• Research use

  • For research use only (per product data). Not for clinical or diagnostic procedures without appropriate validation.

Structure and Identity

• Item-specific identifiers

  • SKU: S1528289 (Aladdin Scientific)
  • Product name: Standard I Nutrient Broth
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey / SMILES: Not applicable (complex mixture; undefined composition of peptides, amino acids, salts, and extracts).

• Nature of material

  • Complex, undefined microbiological growth medium composed of hydrolysates/extracts (e.g., peptones, meat/yeast extract) and inorganic salts to support nonfastidious microorganisms.
  • No single defined small-molecule structure; rather a distribution of oligopeptides, free amino acids, vitamins, nucleotides, carbohydrates, and minerals.

• Structural features (descriptive, literature/general)

  • Predominantly short-chain peptides (2–20 residues) and free amino acids from protein digests providing nitrogen and carbon; small molecules (e.g., amino acids with primary amines and carboxylates), low levels of vitamins, and buffering ions (phosphate, if present in a given formulation) may contribute to pH stability.
  • Inorganic ions (e.g., Na+, Cl−) set osmolarity. Trace metals may be present at low ppm levels depending on raw materials (supplier- and lot-dependent).

Synthetic Utility

Not applicable. Standard I Nutrient Broth is a complex biological medium, not a synthetic reagent or building block.

• Related laboratory utility (general)
  • Serves as a matrix for culturing bacteria that can subsequently be used to produce enzymes, metabolites, or recombinant materials employed in synthesis. However, the broth itself does not participate as a reagent in chemical transformations.

Target Specificity

Not applicable. This product is not a biological probe, antibody, or inhibitor with a defined molecular target. It is a general-purpose growth medium supporting a broad range of nonfastidious microorganisms.