This compound belongs to the class of organic compounds known as strychnos alkaloids. These are alkaloids having a core structure based on the strychnan, stemmadenine (seco-curan), or the akuammicine (curan) skeleton.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Propriétés chimiques et physiques
Poids moléculaire
397.400 g/mol
XLogP3
Hydrogen Bond Donor Count
1
Hydrogen Bond Acceptor Count
6
Rotatable Bond Count
0
Exact Mass
397.164 Da
Monoisotopic Mass
397.164 Da
Topological Polar Surface Area
98.800 Ų
Heavy Atom Count
29
Formal Charge
0
Complexity
714.000
Isotope Atom Count
0
Defined Atom Stereocenter Count
6
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
The total count of all stereochemical bonds
0
Covalently-Bonded Unit Count
2
Calculateurs de solution
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Avis
Avis des clients
Application Protocols
No item-specific, validated protocols (e.g., WB, IHC, IF, FC) are provided for this small-molecule product. Nonetheless, the following general research workflows are commonly reported for strychnine salts; adapt under your institutional safety guidelines:
Electrophysiology (general outline):
Prepare a concentrated stock (e.g., 10 mM) in DMSO or methanol.
Dilute into oxygenated physiological buffer (HEPES- or bicarbonate-buffered saline) to desired working concentration (typically nM–µM range depending on preparation) while keeping final vehicle ≤0.1–1%.
Apply via bath perfusion or local puff; monitor inhibitory postsynaptic currents to confirm glycinergic blockade. Include vehicle controls.
Receptor-binding assays:
Use radioligand displacement or fluorescence-based assays to assess competitive antagonism at GlyRs; define equilibrium times and avoid ligand depletion.
LC/MS calibration (toxicology standard):
Prepare gravimetrically using Class A glassware; verify concentration by an orthogonal method if high accuracy is required.
Store aliquots to limit freeze–thaw and contamination; document whether concentrations are salt- or free-base-equivalent.
These are illustrative research workflows only. Always consult the primary literature and your institutional SOPs, and refer to the product’s CoA/SDS for constraints and safety requirements.
Biological Roles
Strychnine is a classic plant-derived indole alkaloid best known in biochemistry for its potent, competitive antagonism of inhibitory glycine receptors (GlyRs) in the central nervous system. The nitrate salt provides an aqueous-compatible form for laboratory research but does not change intrinsic receptor pharmacology relative to the free base.
Mechanistic role (literature/general):
Competitive antagonist at strychnine-sensitive GlyRs, particularly the α1-containing receptors; binding blocks glycine-mediated chloride influx, leading to neuronal hyperexcitability in biological systems.
Reported off-target interactions at high concentrations with other ligand-gated ion channels have been described; specificity depends on subtype, species, and experimental context.
Experimental use-cases (research only):
Electrophysiology (patch clamp, sharp electrode) to isolate glycinergic inhibitory postsynaptic currents by pharmacological blockade.
Neuropharmacology and receptor-binding assays to characterize GlyR subunit composition and antagonist potency.
As a biochemical tool to differentiate glycine versus GABAergic signaling in mixed inhibitory networks when paired with GABA antagonists.
Handling implications:
Due to extremely high potency, prepare and apply at carefully controlled concentrations; verify final bath or media concentrations and vehicle proportions.
Ensure appropriate deactivation and disposal of waste solutions to prevent environmental or accidental exposure.
Note: All roles described are for laboratory research contexts. No medical or clinical uses are implied or supported.
Buffer Applications
This is not a buffering reagent. Strychnine nitrate does not serve as a pH buffer or common buffer component.
Practical note: When used in biological assays, it is typically dissolved in a compatible vehicle (e.g., DMSO, methanol) and diluted into established physiological buffers (e.g., HEPES-, phosphate-, or bicarbonate-buffered saline). Ensure the final buffer composition (pH, ionic strength) remains unchanged by the vehicle and that the compound remains in solution.
Recommendation: Validate solubility and absence of precipitation after spiking into the chosen buffer at the desired working concentration.
Green Alternatives
As an acutely toxic alkaloid salt used mainly as a biochemical probe/standard, there is no direct “green” substitute that reproduces its specific receptor-binding profile. Nevertheless, greener practices can be applied to its handling and solution preparation.
Substitution considerations:
Mechanistic/assay needs: If the requirement is a glycine receptor antagonist, consider whether less hazardous antagonists with suitable selectivity exist for the particular study design. However, target selectivity and potency may differ substantially; validate pharmacology before substituting.
Analytical standards: Where permissible, use lower-concentration working standards and micro-scale workflows to reduce mass handled.
Greener solvent choices (general):
Prefer water or aqueous alcohols over chlorinated solvents. DMSO and methanol are widely accepted for stocks; avoid unnecessary use of acetonitrile when methanol suffices for LC separations.
Process and waste minimization:
Prepare minimal volumes; adopt on-demand stock preparation to limit disposal of expired solutions.
Utilize closed, low-dead-volume systems for LC/MS to cut solvent use and analyst exposure.
Greener practice: Just-in-time dilute stocks in water:alcohol, micro-aliquoting, validated stability to extend shelf life, and rigorous tracking to prevent redundant preparation.
Always prioritize researcher safety and environmental containment over substitution for target-specific ligands like strychnine.
Pharmaceutical Uses
No excipient or formulation role is intended or implied for this product. Strychnine-containing materials are not used as pharmaceutical excipients due to their extreme toxicity.
Research/manufacturing context only:
May be employed as an analytical reference substance in quality-control or forensic/toxicology laboratories for method development, system suitability, and calibration checks (e.g., LC-UV/LC-MS). Such use requires appropriate certification and documentation.
Pharmacopeial status: Item-specific monographs, if any, are not referenced here. For pharmacopeial or regulatory-grade work, obtain a certified reference material (CRM) and ensure full chain-of-custody and documentation.
Compliance and safety:
Handle under controlled conditions according to institutional and regulatory requirements for acutely toxic compounds.
Maintain rigorous documentation distinguishing salt versus free-base when calculating dosages for spiking solutions in validation protocols.
All uses are restricted to laboratory research or analytical method development. No therapeutic or clinical applications are supported.
Physical Properties
Item-specific numerical specifications (BP, MP, density, solubility constants, refractive index, logP/pKa) are not provided in the Product Data for this listing.
Boiling point: Not applicable for ionic solids; thermal decomposition typically occurs before boiling (general statement for organic nitrate salts).
Melting/decomposition: Not specified for this item; refer to CoA/Spec Sheet. (Literature for strychnine salts often reports decomposition on heating rather than a sharp melt.)
Density: Not specified for this item; refer to CoA/Spec Sheet.
Solubility (general/literature guidance):
Water: Nitrate salts of protonated amines are generally more water-soluble than the corresponding free base; expect moderate aqueous solubility for analytical stock preparation.
Alcohols: Typically soluble in ethanol/methanol.
Nonpolar solvents: Low solubility in hydrocarbons due to ionic character.
pKa/logP: Not specified for this item; refer to CoA/Spec Sheet. (Strychnine contains a basic tertiary amine; protonation drives formation of the nitrate salt.)
Hygroscopicity: Many low-mass alkaloid salts exhibit some hygroscopicity; handle quickly in ambient air and reseal promptly. Item-specific hygroscopic behavior is not specified for this item; refer to CoA/Spec Sheet.
Practical notes (general):
Prepare aqueous or aqueous-alcoholic solutions freshly; filter through 0.22 µm if particulates remain.
If precise concentration-critical work is intended (e.g., toxicology standards), confirm water content and solubility from the lot-specific documentation.
Quality and Grades
Item-specific grade and purity are not provided in the Product Data for this listing.
Grade/Purity (this item): Not specified for this item; refer to CoA/Spec Sheet.
Stabilizers: Not specified for this item; refer to CoA/Spec Sheet. Strychnine nitrate typically does not require added stabilizers under normal storage, but always verify.
What grade terminology generally implies:
Analytical/Reference standard grade: Emphasis on identity confirmation and assay with low-level impurity profiling—typical for toxicology standards. If your application requires trace-impurity limits or certified assay values, request a CoA with chromatographic purity and residual solvent data.
Reagent grade: Suitable for most synthetic and exploratory research applications, but may lack the certification needed for quantitative bioanalytical work.
UV/LC suitability: If intended for chromatographic calibration (e.g., LC-UV, LC-MS), ensure the supplied CoA includes spectral purity, extinction information, and lot-specific assay. For bioanalytical calibration, a certified reference material (CRM) may be preferable when available.
Actions for regulated workflows:
Request detailed CoA including assay methodology, moisture content, counterion content (nitrate), and any verified residual inorganic or organic impurities.
Confirm salt identity versus free base to avoid molarity errors when preparing standards.
Contact Aladdin Scientific to obtain the current lot CoA/Spec Sheet for precise grade, purity, and analytical data.
Reaction and Applications
This compound is primarily used as a biochemical probe/standard rather than as a synthetic reagent. Its nitrate salt form enhances handling for solution-based work.
Research applications (general):
Neurochemistry/pharmacology: Strychnine is a well-established competitive antagonist at glycine receptors; the nitrate salt is commonly used to prepare aqueous-compatible stocks for receptor assays and electrophysiology. Use only in vitro/in situ research contexts; no clinical use.
Analytical/toxicology: Employed as a calibration/positive-control standard in forensic and environmental toxicology workflows. Validate identity and purity against a trusted CoA before quantitation.
Not typically used in:
Classical organic synthesis or as a building block; the complex polycyclic structure and potent bioactivity limit its role as a general reagent.
Practical handling in applications:
Prepare small aliquots at known concentration to minimize repeated handling.
Confirm salt form and report concentrations on a salt- or free-base-equivalent basis consistently.
For electrophysiology or receptor-binding, ensure solutions are free of particulates and that pH/ionic strength remain constant after spiking stocks prepared in DMSO or alcohol.
Methodological notes:
Adsorptive losses can occur on plastic at low nM levels; pre-rinse labware with dilute analyte or use silanized glass when feasible.
Photo- and thermo-stability should be confirmed under assay conditions; prepare fresh on the day of use when high precision is needed.
Reaction Conditions
There are no standard preparative reaction conditions associated with strychnine nitrate, as it is not typically employed as a reagent or substrate in routine transformations. Nevertheless, for solution preparation and analytical workflows, the following general guidance applies:
Stock solution preparation (general):
Solvents: DMSO, methanol, ethanol, or water (depending on required concentration and assay matrix).
Concentrations: Prepare high-concentration stocks (e.g., 1–10 mM) in DMSO or methanol, then dilute into aqueous buffers to nM–µM working ranges according to assay design. Verify solubility at the target level.
Temperature: Room temperature dissolution is usually sufficient; avoid heating that may lead to decomposition.
LC method development (general):
Mobile phases: Water with 0.1% formic or acetic acid; organic modifier methanol or acetonitrile.
Columns: C18 or polar-embedded phases perform well; ensure injection solvent strength is compatible to avoid peak distortion.
Detection: UV in the near-UV (compound exhibits UV absorbance) and MS (positive ESI for the protonated cation) are typical; optimize to avoid detector saturation due to high response at low mass on-column.
Stability considerations:
Protect solutions from prolonged light and elevated temperatures.
Prepare fresh working solutions when quantitative accuracy is required.
For any chemical transformations or derivatizations (rare), consult primary literature specific to strychnine chemistry; no generalized conditions are endorsed here.
Safety and Handling
For research use only. Strychnine salts are extremely potent neurotoxic alkaloids; handle exclusively in appropriately equipped laboratories with trained personnel.
GHS information (item-specific): Not specified for this item; refer to SDS for authoritative classification, H-statements, and pictograms.
Principal hazards (general):
Acute toxicity via oral, inhalation, or dermal exposure; rapid onset of neuromuscular excitation and convulsant effects reported in the toxicology literature for strychnine.
Potential environmental hazard to aquatic organisms; prevent release to the environment.
PPE (general best practice): Lab coat, safety glasses or splash goggles, and double nitrile gloves for weighing/solution prep. Use a chemical fume hood to avoid aerosol or dust inhalation.
Handling tips:
Avoid dust formation; use anti-static tools and weigh boats with covers.
Keep containers tightly closed; do not pipette by mouth; decontaminate surfaces after use.
First-aid overview (non-exhaustive; defer to SDS/medical professionals):
Inhalation: Move to fresh air, monitor respiration; seek immediate medical attention.
Skin/eye contact: Rinse thoroughly with water for at least 15 minutes; remove contaminated clothing; obtain medical attention.
Ingestion: Do not induce vomiting; rinse mouth; seek immediate medical care.
Incompatibilities (general): Strong oxidizers and strong bases/acids may alter composition or accelerate decomposition; avoid excessive heat.
Waste: Collect as acutely toxic organic waste; label clearly and dispose via licensed hazardous waste contractor per institutional and regulatory requirements.
Solvent Selection
Strychnine nitrate is an ionic organic salt; solvent choice should balance solubility, stability, and downstream compatibility.
Polarity and miscibility (general/literature profile):
Water: Enhanced solubility versus the free base due to protonation; suitable for preparing bioassay or analytical stock solutions when buffered appropriately.
Alcohols (MeOH, EtOH): Typically good solubility; useful for LC standards and rapid dissolution prior to aqueous dilution.
Polar aprotics (DMSO, DMF): High solvating power; DMSO stocks are common for receptor/pharmacology studies followed by aqueous dilution.
Nonpolar solvents (hexanes, toluene, ethers): Poor solubility expected due to ionic character.
Selection guidance by application:
Bioassay/ligand-binding: Prepare concentrated stocks in DMSO or methanol, then dilute into physiological buffers maintaining final organic content ≤1–2% to limit vehicle effects. Validate solubility and absence of precipitation.
LC-UV/LC-MS: Methanol:water or acetonitrile:water with 0.1% acid (e.g., formic acid) commonly used; verify detector linearity and avoid on-column overload.
Solid-state manipulations: Minimize aqueous exposure if crystallinity must be preserved; dry alcohols allow rapid dissolution and evaporation.
Practical tips:
Filter 0.22 µm to remove particulates before injection or bioassay.
Avoid strong basic solvents that could deprotonate the salt and alter solubility.
Record whether concentrations are reported as salt or free-base equivalents to ensure inter-study comparability.
Storage and Reconstitution
Storage conditions provided for this item: Room temperature.
Storage (this item):
Keep tightly closed at room temperature in a dry, well-ventilated place. Protect from excessive heat and direct light. Store in secondary containment appropriate for acutely toxic substances.
Shipped in: Not specified for this item; refer to CoA/Spec Sheet.
Stability notes (general):
Solid alkaloid salts are typically stable at ambient conditions when protected from moisture and light. Item-specific shelf life is not specified; consult the CoA for retest/expiry.
Reconstitution (general guidance):
Solvents: DMSO, methanol, ethanol, or water depending on required concentration and downstream use.
Procedure: Allow the vial to equilibrate to room temperature before opening to prevent moisture condensation. Weigh rapidly to minimize exposure. Dissolve with gentle swirling—avoid heat. Filter (0.22 µm) if clarity is required for analytical or biological assays.
Aliquoting: Prepare single-use aliquots to avoid repeated opening. Clearly label with concentration, solvent, date, and whether values are expressed as salt or free-base equivalents.
Freeze–thaw:
Solid: Not applicable. Solutions: Minimize freeze–thaw cycles; store working stocks at 2–8°C for short term or at −20°C for longer term if solvent-compatible, and verify stability before use.
Always refer to the lot-specific CoA and SDS for definitive storage stability and handling instructions.
Structure and Identity
Strychnine nitrate is the nitric acid salt of the polycyclic indole alkaloid strychnine. As a protonated tertiary amine paired with nitrate, it features a dense, rigid scaffold with multiple fused rings and a basic nitrogen rendered cationic in the salt form.
SKU: S1021129
Product name: Strychnine Nitrate
CAS: 66-32-0 (as provided for this item)
InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight (item-specific): Not specified for this item; refer to CoA/Spec Sheet.
Structural features (general/literature description):
Polycyclic cage-like alkaloid based on an indole/indolizidine-like fused-ring framework, containing a tertiary amine (protonated in the nitrate salt), an amide/ester-like carbonyl within the bicyclic system, and multiple stereogenic centers (strychnine is highly stereodefined in nature).
The nitrate counterion (NO3−) balances the cationic ammonium center formed by protonation of the strychnine nitrogen, improving crystallinity and aqueous compatibility versus the free base.
2D description: a compact, multi-ring cation with one positively charged tertiary amine nitrogen; a nitrate anion as a discrete counterion; no extended conjugation despite an embedded indole-like substructure due to extensive ring fusion and saturation.
Notes:
Where precise identifiers (SMILES, InChIKey for the salt) are required, consult the CoA/Spec Sheet for this specific lot to avoid ambiguity between the base and its salts.
Synthetic Utility
Strychnine nitrate is not generally leveraged as a building block or reagent in preparative organic synthesis due to its complex, densely functionalized, and highly potent alkaloid framework.
Functional group context (general):
Tertiary amine (protonated in the nitrate salt), embedded carbonyl functionality, and multiple stereocenters within a rigid polycyclic architecture.
The nitrate serves as a non-nucleophilic counterion; it is not typically exploited in downstream transformations.
Why it is not commonly used synthetically:
Hazard profile and handling restrictions severely limit multi-gram manipulations.
Lack of obvious points for selective derivatization without disrupting the polycyclic core; classical semisynthetic transformations are limited and specialized.
Where it may appear:
As a structural probe in binding studies or crystallography of ligand-gated ion channels (ligand role), not as a reagent.
Occasionally as a benchmark molecule in total synthesis and stereochemical studies (literature), but the nitrate salt itself is not a typical starting material for such endeavors.
Conclusion: Treat this compound as a specialized biochemical ligand/standard. For synthetic needs involving cationic, water-compatible amines, consider benign surrogates specifically designed for derivatization rather than using strychnine salts.
Target Specificity
Item-specific biological target data such as antigen/epitope, species reactivity, clone, or isotype are not applicable—this product is a small-molecule alkaloid salt, not an antibody or biologic.
General/literature context:
Strychnine is widely cited as a potent, competitive antagonist at strychnine-sensitive glycine receptors (GlyRs). Specificity and potency can vary by receptor subunit composition and assay conditions. No claims are made here for quantitative affinity values for this item; consult primary literature for detailed pharmacology.
For your experimental system, validate target engagement empirically using appropriate positive/negative controls and orthogonal assays.
Foire aux questions
How should this product be stored?
Store at room temperature.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 66-32-0. InChIKey PCGVPMHGSJFFTI-ZEYGOCRCSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Nous utilisons des cookies pour garantir le bon fonctionnement du site et, lorsque cela est autorisé, pour améliorer votre expérience. Vous pouvez gérer vos préférences à tout moment dans les Paramètres. Pour en savoir plus, consultez notre Politique relative aux cookies.
Souhaitez-vous que nous vous envoyions un message lorsque des réductions seront disponibles?
Me rappeler plus tard
Merci ! Veuillez vérifier votre boîte de réception e-mail pour confirmer.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
