TMB PLUS2 - Ready-to-use (RTU)

Cat. No.: T494080

GRADE & PURITY Ready-to-use (RTU)

Synonyms

TMB

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Vue d’ensemble

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride)

TMB PLUS2 characteristics are:

Medium sensitivity

Very low background

Aqueous based – no organic solvents (REACH Annex XVII compliant)

Low lot-to-lot variation

For traceable pipetting, we recommend using pink colored TMB PLUS2 Prestained (cat. no. T494080-01). The pink indicator disappears after addition of the stop solution.

Product Characteristics 

TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm.

TMB PLUS2 is a ready-to-use, very active and stable aqueous formulation and this substrate gives higher signal than standard TMB solutions ensuring a very fast assay reaction. It does not contain NMP (1-methyl-2-pyrrolidone) making it REACH Restricted Substances List Annex XVII compliant, while ensuring maximal safety during use, and minimal negative environmental impact.

Composition & Properties 

Ready-to-use substrate: Includes substrate buffer and hydrogen peroxide. No other reagents should be added.

Working Procedure

1.The desired amount of substrate is poured into a sealed container and allowed to reach room temperature in the dark before use.

2.A recommended volume of 100 µl substrate solution per microtiter well is added. Time of development is typically 5-30 minutes. Keep the plate in the dark during incubation.

3.In kinetic assays, the blue color is read directly at 650 nm. For endpoint assays 100 µl (equal volumes) of 0.20 M sulphuric acid is added and the absorbance is read at 450/620 nm. The yellow solution is stable for at least one hour.

Tips & Tricks 

• If reduced intensity is required, it is recommended to shorten the time of development or to dilute the conjugate/antibody in the immunoassay further. 

• Dilution of the substrate is not recommended unless a corresponding TMB PLUS2 Diluent is acquired. The diluted TMB solution maintains the same stability performance as the undiluted version, and a specific activity can be achieved. 

• For traceable pipetting, we recommend using the pink colored TMB PLUS2 Prestained (cat. no. T494080-01). The pink indicator disappears after addition of the stop solution. 

• In case of incidents of precipitation with very high OD-values, we recommend the use of the TMB X-tnd (cat. no. T494100)

Handling & Storage 

• Store solution at 2-8 °C in the dark. 

• Avoid exposure to light, heat and contamination with metal ions or peroxidase. 

• Re-dispense only into bottles made of High Density Polyethylene (HDPE), amber color. Dispensing guidelines are available upon request.

Product specifications and parameters

SKU	Product name	Product Type	Pack Size
T494080-01-100ml	TMB PLUS2	Prestained	100ml
T494080-01-500ml	TMB PLUS2	Prestained	500ml
T494080-01-1L	TMB PLUS2	Prestained	1L
T494080-02-100ml	TMB PLUS2	Not prestained	100ml
T494080-02-500ml	TMB PLUS2	Not prestained	500ml
T494080-02-1L	TMB PLUS2	Not prestained	1L

Specifications

Synonymes

TMB

Spécifications et pureté

Ready-to-use (RTU)

Conditions de stockage de stockage

Store at 2-8°C,Protected from light

Expédié en

Wet ice

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Images

TMB PLUS2