Tomeglovir - 10mM in DMSO , CAS No.233254-24-5

CAS: 233254-24-5 Cat. No.: T656876 Formule: C₂₃H₂₇N₃O₄S Poids moléculaire: 441.54 PubChem CID: 475330

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

Tomeglovir is a potent anti- CMV agent, inhibiting processing of viral DNA-concatemers, with IC 50 s of 0.34 μM and 0.039 μM for HCMV and MCMV.

In Vitro

Tomeglovir (BAY 38-4766) is a potent anti-CMV agent, with IC 50 s of 0.34 μM and 0.039 μM for HCMV and MCMV. Tomeglovir also suppresses HELF and NIH 3T3 cells, with CC 50 s of 85 μM and 62.5 μM, respectively. Tomeglovir (BAY 38-4766) inhibits HCMV Davis and various monkey CMV strains with EC 50 s of 1.03 ± 0.57 μM and < 1 μM. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tomeglovir (BAY 38-4766; 3, 10, 30, 100 mg/kg, p.o.) dose-dependently reduces MCMV-DNA in salivary glands, livers and kidneys of MCMV-infected NOD-SCID mice, and prolongs the survival of the mice. Tomeglovir (10, 25 and 50 mg/kg) shows antiviral activity in the hollow fiber mouse model . Tomeglovir (BAY 38-4766) shows antiviral activity in SCID mice with MCMV, and the LD 50 is >2000 mg/kg in mice and rats. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay

In order to evaluate drug toxicity, 96-well microtitre plates are prepared with 100 μL of EMEM/10 per well. After addition of 2 μL of 50 mM Tomeglovir stock solutions in duplicate into 198 μL in row 2, serial two-fold dilutions are made with 100 μL up to row 12 and 100 μL of a HELF, NHDF or 3T3 cell suspension (5 × 10 3 cells/mL) are added per well. Row 1 serves as an untreated cell control. After incubation for 6 days at 37°C and 5% CO 2 , the cells are washed once with phosphate-buffered saline (PBS), and 200 μL of a 10 μg/mL fluorescent dye solution in PBS, pH 7.2 (fluorescein diacetate) are dispensed per well. After 45 min, the fluorescence signal is measured with a Fluorskan Ascent fluorimeter (excitation filter 485 ± 11 nm, emission filter 530 ± 15 nm). The relative fluorescence units (RFUs) of treated cells are expressed as percentages of untreated cell controls and CC 50 values are determined graphically. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:IC50: 0.34 μM (HCMV), 0.039 μM (MCMV)

Specifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

Tomeglovir is a potent anti- CMV agent, inhibiting processing of viral DNA-concatemers, with IC 50 s of 0.34 μM and 0.039 μM for HCMV and MCMV.

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Noms et identifiants

Isomères SMILES

CC(C)(CO)C(=O)NC1=CC=C(C=C1)NS(=O)(=O)C2=CC=CC3=C2C=CC=C3N(C)C

PubChem CID

475330

Poids moléculaire

441.54