GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Vue d’ensemble
Trypsin selectively hydrolyzes peptide bonds formed by the carboxyl groups of lysine or arginine in denatured proteins, making it an important digestive enzyme. Additionally, trypsin is widely used as an adjunct therapy for conditions such as local edema, hematomas, and abscesses resulting from empyema, hemothorax, surgical inflammation, ulcers, traumatic injuries, and similar cases. In this assay kit, trypsin catalyzes the hydrolysis of the ester bond in TAME, releasing free carboxyl groups. These groups undergo a neutralization reaction with sodium hydroxide present in the reaction system, leading to a decrease in the pH of the solution. Using phenol red as an indicator, the change in absorbance at 555 nm is measured, enabling rapid determination of trypsin activity. Within a certain range, trypsin activity exhibits a well-defined linear relationship with the decrease in absorbance at 555 nm.
Specifications
Spécifications et pureté
BioReagent
Grade
BioReagent
Application
Enzyme activity assay
Type d'échantillon
Tissus animaux, Tissus végétaux
Méthode de détection
Colorimetric Method
Detection instrument
Microplate reader,Visible Spectrophotometer
Detection wavelength
555nm
Stockage et expédition
Conditions de stockage de stockage
Store at 2-8°C,Protected from light
Expédié en
Wet ice
Stabilité et stockage
Store at 2-8℃ for 6 months. Store in the dark.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
